On which of the following types of media would you expect untransformed bacteria to grow on?

Q: Bacterial growth depends on many environmental factors, including the temperature of the…

A: The aseptic technique is a method used to prevent the contamination of the culture from the…

Q: Isolating a pure culture from a mix sample is typically done on solid agar but can be performed…

A: A microbial culture is a method of multiplying microbial organisms by letting them reproduce in…

Q: Why do cells need to be kept in the freezing devices first then transferred to liquid nitrogen…

A: Rapid freezing of cells can cause ice crystals large crystals can be formed in which cells are…

Q: An attempt to transfer bacteria into new media during the death (Decline) phase of a culture…

A: Microbial cultures, also known as microbiological cultures, are made by allowing microbial organisms…

Q: You then take a cotton swab and subculture a sample from this zone of inhibition (where no growth…

A: The Kirby-Bauer test, known as the disk-diffusion method, is the most widely used antibiotic…

Q: You inoculate a species of bacteria on a MacConkey agar which normally shows positive growth and the…

A: MacConkey Agar (MAC) is selective and differential agar that is used to isolate non-fastidious…

Q: Collecting data that can then be transformed into a bacterial growth curve is usually done using a…

A: A spectrophotometer is an instrument used to measure the amount of light absorbed by a sample. The…

Q: All of the following are correctly matched and can be found in bacteria EXCEPT: None of the other…

A: Note : Dear student as per our guidelines we are supposed to answer only one of your questions. So…

Q: Why is a metal loop heat to read before picking up a bacterial sample? What should you do with a…

A: In microbiology, inoculation is the technique of transferring bacteria into culture media so that…

Q: Can culture media be placed in a freezer? Why or why not

A: Culture media are also called as growth media. They are mixture of nutrients and other substances…

Q: Imagine that you are Gram staining a mixed culture of both gram-positive and gram-negative bacteria.…

A: The bacteria cell envelope is a complex multilayered structure and based on this structure the…

Q: Dilute cells from an old culture 1:200 into 5 mL of fresh media. What volume of the old culture…

A: Introduction Dilution is the process of lowering the concentration of a solute in a solution by…

Q: Mannitol salt agar is a selective media that inhibits the growth of Gram-negative bacteria due to…

A: A Growth medium is an environment created by combining various nutrients that support the growth of…

Q: For growth, a mutant bacterium requires that an amino acid not required by the wild parental strain…

A: Answer :- For growth, a mutant bacterium requires that an amino acid not required by the wild…

Q: A researcher is planning to plant antibiotic-resistant Escherichia coli pure bacteria sample in…

A: Culturing the bacteria on a solid media involves several steps in which the major step is…

Q: What type of agar would you use to grow gram positive bacteria

A: Bacteria are different from the plant as well as animals. They require specific substances in the…

Q: A growth medium consisting of only known amounts of water, magnesium, calcium chloride, potassium…

A: The growth medium, also called as Nutrient Broth, is a kind of solution which is sterilized to get a…

Q: Indicate which of the following observations can be made when observing growth on nutrient agar.…

A: Colony morphology can be referred to as the visual appearance of bacterial colonies on a plate of…

Q: In the diagrams above, an Electron Transport Chain (ETC) was depicted. ETCS contribute to the…

A: The process of forming ATP from the electron transport chain is known as oxidative phosphorylation.…

Q: Which of the following are true of the above picture? (Select all that apply.) V The bacteria are…

A: Gram-negative bacteria have a thin peptidoglycan layer which fails to retain colour after treatment…

Q: The wild type bacterium is lac+ amps, leu+, ade+ Mutant I is: lac+ amp r, leu-, ade+ Mutant 2 is:…

A: Following the addition of cells with the designated genotypes and enabling growth to take place,…

Q: In August 2021, there was a multi-state outbreak of melioidosis, an infectious disease caused by the…

A: In microbiology, there have different types of culture mediums that are commonly used to grow…

Q: Why is PBS buffer preferred when preparing a monolayer cell culture?

A: PBS (phosphate buffer saline) is a buffer solution which is a water based salt solution containing…

Q: Before viewing a specimen of pigmented bacteria on a slide under the light microscope, which of the…

A: The wet mount is the most basic method of preparation, in which the specimen is placed on the slide…

Q: You have a broth medium that you would like to turn into a solid medium in order to do streak…

A: The "nutrient broth" is essentially nutrient agar without the solidifying agent. They stay liquid at…

Q: List out 4 environmental factors (ie, temperature, pH level, water availability) that affect…

A: Bacterial growth occurs when a bacterium divides into two daughter cells, a phenomenon known as…

Q: Bacterial cells can form colonies on the following media EXCEPT: Select one: Nutrient broth Agar…

A: The nutrient broth is the liquid nutrient media that lacks the solidifying agent. It is used for…

Q: Which of the following media selects for gram negative bacteria by inhibiting the growth of gram…

A: Selective media:- it usually allow the growth of a particular microbe and inhibit the growth of…

Q: Your animal cell culture laboratory suddenly suffered from a bacterial contamination. Several…

A: B. The media is probably contaminated with Mycoplasma, due to the lack of cell walls they are the…

Q: What is growth in "pure culture"? O Growth of human pathogens Growth of just one species in the lab…

A: A microbial culture is a method of multiplying microbial organisms by letting them reproduce in a…

Q: Which of the following is NOT correctly matched?   A. Halophile – microbe that can grow in an…

A: All the above represents  match the term questions

Q: If you have a 15 mg/100 ml stock solution of GA3 and you need a 1 mg GA3 in 25 ml, how much stock…

A: Given: volume of stock solution is 15 mg/100 ml Needed amount is 1 mg kinetin in 25 ml Volume of…

Q: Cellular stains are commonly used in bacteriology. Which of the following is NOT a reason to stain a…

A: Introduction :- Cell staining is a method that can be applied to improve microscopic views of cells…

Q: (b) In a batch bioreactor, the growth of a microbial population is often limited by the availability…

A: As the question contain two different questions, here we have only solved the second one. Bacteria…

Q: You inoculate a species of bacteria on a MacConkey agar. It normally shows positive for growth and…

A: MacConkey Agar is a differential agar for Lactose fermentation while if no other Sugars are used,…

Q: Describe how to most easily calculate the generation time for a population of bacteria in a batch…

A: Bacteria grow when one bacterial cell divides to form two daughter cells. Thus, bacterial growth can…

Q: What color would the bacterial cells have when viewed microscopically after the following treatment?…

A:

Q: You have 2 mL of bacterial culture. How would you make a 10^-2 dilution using the entire culture…

A: The objective of this question is to understand how to make a 10^-2 dilution of a bacterial culture…

Q: You are working through the Gram Staining procedure on a mixed culture of S. aureus (G+) and E. coli…

A: During the Gram staining procedure the crystal violet dye is applied to the bacterial cells followed…

Q: The first step in identifying an unknown bacterium is to __________. culture your unknown on…

A: Bacteria are the minute microorganisms that are unicellular and have a cellular level of…

Q: In an experiment, the bacteria were placed in dropper bottles containing glycerol as a carbon…

A: The purpose is to kill any previous bacteria on the loop before it is exposed to the bacteria. The…

Q: In a batch reactor with an optimal culture medium, which of the following growth-phase sequences is…

A: Bacterial growth occurs rapidly in a favorable environment where all the necessary conditions for…

Q: What type of agar would you use to grow gram negative bacteria

A: Gram-negative bacteria are the bacteria that do not consist of the crystal violet stain and they are…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.