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Bacterial cells can form colonies on the following media EXCEPT:
Nutrient broth
Agar slant
Agar plates
Agar deep
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- Which of the following media selects for gram negative bacteria by inhibiting the growth of gram positives that typically incorporate its dye component into their relatively bigger cell walls? blood agar EMB Mannitol salt agar Simmon citrate agar urease brothDefine the term “colony” as it relates to bacterial growth on solid media.What is the major difference between an enrichment culture and a selective culture? Why are microbial doubling times in nature typically longer than those obtained in the lab? Briefly describe the following mechanisms of measuring bacterial growth: Direct microscopic cell count Plate count Most probable number
- MSA (Mannitol Salt Agar) Match the description to the correct Bacterium A= growth, agar is yellow B= Growth, agar is not yellow C= no growthIf lysozyme is added to a microbial culture. Predict what will be observed if the culture is affected by the action of lysozyme. A change in color will be visible in the tube. An increase in absorbance will be observed over time. A decrease in absorbance will be observed over time. An increase in turbidity will be observed over time.Describe characteristics of Streptococcus Agalactiae in the Agar: (How does colonies look like (color) and explain does it grow on that agar. (Don't have to write the incubation period) ~ Only describe how would it look like on the Agar: Blood Agar (Aerobic) MacConkey EMB PEA Mannitol Salt Agar Chocolate Agar Nutrient Agar
- On which of the following types of media would you expect untransformed bacteria to grow on? LB agar LB agar with ampicillin Untransformed bacteria will not grow on any type of medium. Untransformed bacteria will grow on all three types of media. LB agar with ampicillin and arabinoseA microbe is growing on Sabouraud dextrose agar, which has a slightly acidic pH. Which of the following is this microbe likely to be? a Gram-negative bacterium an acid-fast bacterium a fungus a Gram-positive bacteriumIn the laboratory, autoclaving is required to prepare media for the growth of microorganism. What type of method of control does this involve? Dry Heat Moist Heat Chemical Radiation All of the options
- Which of the following statements regarding microbial control agents is FALSE? Question options: strongly acidic or alkaline disinfectants are typically more effective than neutral pH agents the presence of extracellular fats typically decreases the effectiveness of control agents a warmed disinfectant is typically more effective than the same disinfectant at room temperature substances secreted by members of biofilms increase protection against microbial control agents all of these statements are TRUE bacterial endospores are more resistant to antimicrobial treatment than vegetative bacterial cellsA culture of E. coli has a concentration of 5 x 108cells/mL. How many times do you have todilute the culture so that when you spread 0.1mL on an agar plate you will have 250 colonies?Hint: you need to find dtotal and then convert it into a DF.Put the following Steps of gram smear in order Create a bacteria Add crystal violet to the bacterial smear. Rinse with water after 60 seconds. Allow the smear to air dry and then heat fix it. Add gram iodine to the smear, let it sit for 60 seconds and rinse water decolorize with 95% ethanolfoe 5-10 seconds and then rise with water Blot dry with bibulous paper Add safranin to the smear and let sit for 60 seconds, then rinse with water Observe using oil immersion