Which of the following tools of DNA technology is incorrectlypaired with its use?(A) electrophoresis—separation of DNA fragments(B) DNA ligase—cutting DNA, creating sticky ends of restriction fragments(C) DNA polymerase—polymerase chain reaction to amplifysections of DNA(D) reverse transcriptase—production of cDNA frommRNA

Match each enzyme name in the left column with the correct descriptive phrase in the right column. (a) Topoisomerase II(b) DNA ligase(c) DNA polymerase γ(d) Reverse transcriptase(e) DNA polymerase I(f) DNA polymerase III i. Catalyzes most nucleotide incorporations in bacterial DNA replicationii. Cleaves RNA in a DNA–RNA hybrid moleculeiii. Uses a tRNA primer in synthesis of retroviral DNAiv. Acts through an adenylylated DNA intermediatev. Catalyzes formation of a double-strand DNA breakvi. Catalyzes mitochondrial DNA replication

Quantitative PCR differs from regular PCR in that it uses [A] to [B] the amount of [C] in a sample. It cannot quantify [D] unless it is first made into [F]. Match each of the following to its appropriate letter: quantify, cDNA, RNA, DNA or RNA, fluorescence. 1) A 2) B 3) C 4) D 5) E Here are the choices for the questions a) quantify b) RNA c) cDNA d) fluorescence e) DNA or RNA

Which of the following statements about PCR or RT-gPCR is correct? 1. Fluorescence is detected when the TagMan probe is cleaved by Taq polymerase (AMPLITAQ). 2. PCR uses the natural process of transcription to amplify DNA. 3. Both PCR and RT-qPCR require forward and reverse primers. 4. Fluorescence is detected when the reporter dye is in close proximity to the quencher dye. A. 1, 2 and 3 B. 1 and 3 C. 2 and 4 D. 4 only E. All of 1, 2, 3 and 4 are correct

The primer is needed during DNA replication because: A) it provides energy for making the new strand B) actually, primer is NOT needed because DNA polymerase can make DNA "from scratch" C) it helps DNA polymerase to bind to the DNA D) it serves as an existing nucleic acid to which DNA polymerase can add nucleotides to form the new strand E) it serves as the template for making the new strand

The wrong nucleotide is occasionally incorporated during DNA replication and not immediately repaired by the proofreading function of DNA polymerase. Which of the following DNA repair systems relies upon methylated DNA to determine which base is correct and which needs to be repaired?a) CPD photolyase repair b) mismatch repairc) nucleotide-excision repair d) base-excision repaire) homology-directed repair