Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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The primer is needed during DNA replication because:
A) it provides energy for making the new strand
B) actually, primer is NOT needed because DNA polymerase can make DNA "from scratch"
C) it helps DNA polymerase to bind to the DNA
D) it serves as an existing nucleic acid to which DNA polymerase can add nucleotides to form the new strand
E) it serves as the template for making the new strand
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- What holds the two strands of a DNA double helix together? A) hydrogen bonds between bases B) hydrogen bonds between phosphates C) hydrophobic bonds between bases D) hydrophobic bonds between sugars E) phosphate bondsarrow_forwardWhich of the following statements is false about DNA replication? A) Each lagging strand of DNA is started by an RNA primer. B) DNA polymerase joins nucleotides in the 5' to 3' direction only. C) The leading strand of DNA is made continuously. D) More than one replication fork is present. E) A replication bubble opens in one direction only.arrow_forwardA DNA strand was sequenced using the Sanger method (https://www.youtube.com/watch?v=KTstRrDTmWI). The reaction tube contained the DNA strand, fluorescently labelled dideoxynucleotide triphosphates (ddATP – yellow, ddGTP – green, ddCTP – blue, ddTTP - red), deoxynucleotide triphosphates, DNA polymerase, or its Klenow fragment. Synthesis of DNA is allowed to proceed, and the results are shown on the right: 15 14 13 12 11 10 (a) What is the sequence of the copy and the template strands? (b) If the template strand were in the 5'-3' direction, what will be the sequence of the DNA copy? Nucleotide Lengtharrow_forward
- DNA polymerase occasionally incorporates the wrong nucleotide during DNA replication. If left unrepaired, the base-pair mismatch that results will lead to mutation in the next replication. As part of a template strand, the incorporated wrong base will direct the incorporation of a base complementary to itself, so the bases on both strands of the DNA at that position will now be different from what they were before the mismatch event. The MER-minus strain of yeast does not have a functional mismatch excision repair system, but it has normal base excision repair and nucleotide excision repair systems. Which of the following statements is correct about differences in the mutation spectrum between MER-minus and wildtype yeast? More than one answer is correct. Options: More point mutations will arise in MER-minus yeast. Fewer point mutations will arise in MER-minus yeast as compared with wildtype. Of the total point mutations that…arrow_forwardWhich of the following is true about restriction endonucleases?a) Type I and II requires ATP to move along DNAb) Type I, II and III requires ATP to move along DNAc) Type II requires no ATP and cleaves DNA within recognition sequenced) Type II requires ATP and cleaves DNA within recognition sequencearrow_forwardOnce the strands have been replicated, the enzyme DNA polymerase I “cleans up” by removing the primers. Why must the primers be removed? Finally, the enzyme DNA ligase finishes up. What does DNA ligase do?arrow_forward
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