In PCR, the size of the product is based on A the position of the primers. B) the amount of DNA you add to the reaction. c) the number of cycles the process has gone through. the amount of enzyme you add to the reaction. E) the location of the machine.

Which of the following does NOT require a primer? A) PCR-based viral test B) cloning a human gene from mRNA C) subcloning a bacterial gene from one plasmid to another D) DNA fingerprinting for paternity testing E) DNA sequencing

Describe how Sanger sequencing works. Make sure to mention dNTPs, ddNTPS, template strand, primers:

Choose the correct statements from the list below. There may be more than one correct statement. A) If you start with 2 DNA templates, after four rounds of PCR you'll have 32 copies B) PCR is useful in making millions or billions of copies of a gene so that it is present in a quantity large enough to study C) quantitative PCR is very similar to PCR, but fluorescent probes are added so that we can measure how much PCR product exists by examining how much the reaction fluoresces D) In real-time reverse transcriptase PCR, the RNA is used as a template to make a cDNA copy (through reverse transcriptase)

TEIIF Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a a) comes to the complex with the polymerase b b) loosens non-specific interactions with the DNA c) both a and b d) none of the above