Which of the following statements is not true? Explain why. A. A DNA strand can serve as a template strand on many occasions. B. Following semiconservative DNA replication, one strand is a newly made daughter strand and the other strand is a parental strand. C. A DNA double helix may contain two strands of DNA that were made at the same time. D. A DNA double helix obeys the AT/GC rule. E. A DNA double helix could contain one strand that is 10 generations older than its complementary strand.

What is true of this figure? (can be multiple answers) a. the replication fork is asymmetrical b. the DNA strands are anti-parallel c. one strand runs 3’ to 5’ d. one strand runs 5’ to 3’ e. both strands have identical bases

Some antibiotic drugs fight infection by interfering with DNA replication, transcription, or translation in bacteria. Indicate whether each of the following antibiotic drug effects is on replication, transcription, or translation. HINT Each answer (replication, transcription, and translation) is used only once for the following: a. Rifampin binds to bacterial RNA polymerase. b. Streptomycin binds bacterial ribosomes, disabling them. c. Quinolone blocks an enzyme that prevents bacterial DNA from unwinding.

Consider the following segment of DNA, which is part of a linear chromosome: LEFT            5’.…TGACTGACAGTC….3’ 3’.…ACTGACTGTCAG….5’                    RIGHT   During DNA replication, this double-strand molecule is separated from the right to the left into two single strands and the replisome is moving from the right to the left of the segment. ___________ should be the template for the lagging strand synthesis.     neither of the two strands   the bottom strand   both top and bottom strands   the top strand

Consider Figure 7, which illustrates a point in DNA synthesis for one end of a chromosome, after the lengths of the chromosomes have been replicated, but before telomerase has functioned. (The other end of the chromosome is not shown. A and C mark the midpoint of the chromosome). Each long rectangle represents one strand of DNA. 1. State the locations below as the 5’ or 3’ ends. Location labeled B is: 5’ 3’ Not possible to determine and I have answered below. Location labeled D is: 5’ 3’ None of the above and I have explained my reasoning here.

A diagram of a prokaryotic replication fork is shown.  As in class, RNA primers are represented by a wavy line and DNA represented by a straight line.  From the list below pick all the statements that are TRUE of this replication fork.  (Multiple answers possible) A.Primer sequences are not correct. B. DNA being synthesized is not complementary and antiparallel to the template. C. Direction of DNA synthesis is not correct (not 5' to 3'). D. At least one protein in the diagram is mislabeled.

During replication of a DNA molecule, the daughter strands are not produced in exactly the same manner. One strand, the leading strand, is made continuously toward the replication fork, while the lagging strand is made in fragments in the opposite direction. This difference in synthesis of the two strands is the result of which of the following? O a. DNA polymerase is not efficient enough to make two "good" strands of DNA O b. The two template strands are antiparallel, and DNA polymerase can only synthesize DNA in the 5' to 3' direction O c. The lagging strand is the result of DNA breaks caused by exposure to UV light Od. The cell does not contain enough nucleotides to make two complete strands Oe. Both B&D

During template-directed synthesis of a new DNA strand it can happen, if there are simple repeated sequences, that either the template strand or the strand being synthesized "slips" a short distance, and this can change the number of repeating sequence units in that stretch of repeated sequence. Which of the following processes involve such slippage? More than one answer is correct.  Options:   The increase in genomic copy number of a DNA transposon by transposition from a location behind a replication fork to a location ahead of the fork.   Introduction of indels during DNA replication.   The initial unwinding of the DNA duplex during replication by helicase.   Increasing lengths of CAG trinucleotide repeats in the huntingtin gene giving rise to Huntington disease.   Synthesis of primer by primase during DNA replication.

Which of the following statements is FALSE regarding the molecular mechanism for DNA polymerases?   A. The active site contains 2 divalent metal ions   B. A single stranded DNA template is required   C. The enzyme can only attach a new deoxynucleotide to the 5’ end of a growing chain   D. The 3’OH on the deoyxyribose ring attacks a phosphate of a dNTP to produce a new phosophodiester bond   E. None of the above (all are true statements)

There are several factors that contribute to high fidelity of DNA replication. Which of the following statements is INCORRECT? DNA polymerase I has a 3'-5' exonuclease editing activity. O b. RNA primers are used instead of DNA primers. There are balanced levels of dNTPs inside the cell. O C. O d. DNA polymerase III has a 3'-5' exonuclease editing activity. O e. Base-pair geometry plays important role for maintaining the high fidelity of DNA synthesis.