While conducting the Hydrogen Sulfide Test (SIM Agar) you open up one of the tubes and it gives off a rotten egg smell while another tube you cultured does not. What does the smell indicate? How come there was no change in color in the tube that indicated a positive test? Please explain how the color change is to occur. Think critically.

Discarding dirty, used micropipette tips is one way to practice sterile technique. What are some other ways to practice sterile techique in lab this week? Check all that apply. Wear gloves while working with the EcoPlate, micropipette, and soil serial dilutions. Leave your sterile tubes uncapped and open, for airborne bacteria to enter. Keep food and drink off your lab bench. Remove your gloves before handling your computer, phone, etc. Use provided ethanol solution to clean your lab bench. Touch the soil or soil solutions with your bare hands or fingers.

Out of the three gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, and Staphylococcus epidermidis).  Which of the following test(s) will help me identify the unknown gram-positive bacteria?     Gram Stain Wet Mount (motility testing) Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Indole Production Citrate Utilization Triple Sugar Iron Testing Catalase Test

Match the confirmation test to the category of bacteria. (each category can be used multiple times)  Categories: -Gram Positive  - Gram Negative  Tests: Match with category  A. Citrate test is category.... B. Indole test is category...  C. Raffinose test is category... D. Catalase test is category....

A positive ELISA assay usually results in __________.   decreased motility fluorescence agglutination a color change visible bubbling

What is an antigen?  What is an antibody? Why does each test include a control line? What specimen would be collected for an antigen test? Why is it important to not touch the tip of the swab?What specimen would be collected for an antibody test? How many lines for a positive antigen test?How many lines for a negative antigen test? What do three lines show in an antibody test?

a) did the positive and negative controls work correctly in this ELISA? Explain.     B) in the sample wells( wells3-7) some are darker blue than others. Explain what happened here and how you'd interpret the results?

What is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide  2. To cause the cells to shrink and become distorted 3. To kill the bacteria so they aren't harmed by the staining method 4. To break down the cell wall in order to make the cells accept stain 5. To kill the bacteria to make the slide safer to handle

What test can help you differentiate staphylococci from streptococci? What reagent or media would be use? short answer only

Which of the following is/are TRUE 1. regarding microscopes? * Which of the following is/are TRUE 2. regarding the use of laboratory glass wares and instruments? * Brightfield microscopes, a compound microscope, can be used to examine stained and unstained specimen. Both the inoculating loop and inoculating needle can be used in nutrient broth. AFM uses a diamond and a metal probe that scans surfaces of a specimen A serologic pipette has a bulbous part which is used to deliver different volumes of liquid. Darkfield microscope has an opaque disc which brightens the light that would enter A glass slide and coverslip are to observe the lenses. stained microorganisms. 4. Which of the following are TRUE regarding Which the following is/are TRUE 3. |regarding microscopes? * the use of laboratory instruments and equipment in regards to making of culture media? TEM gives excellent view of internal structures of a specimen in three- dimensional image Air bubbles on the surface of agar plates…