Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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Which of the following can be a achieved by CRISPR/Cas9-based genome editing tool?
A. Knocking out one or more gene
B. Activating expression of a gene
C. Suppressing expression of a gene
D. All of the above
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- Unusually high levels of Mdr1 provide stem cells with a. Nutrients needed b. Protection of their genomes from mutagenic agents c. Apoptotic factors d. Ability to differentiatearrow_forwardFollowing a CRISPR mediated DNA double-strand break, how can the DNA be repaired by the cell? Choose all that apply. A. Base pairing between complementary sticky sides. B. Nonhomologous end joining. C. Homology directed repair. D. Crossing over between nonhomologous chromosomes.arrow_forwardDescribe a biotechnology technique(s) that you could use to study how active (how much transcription is occurring) in one specific gene.arrow_forward
- Consider the reagents required to perform CRISPR genome editing. Which molecule specifies the a new sequence that will occur at the target site if genome editing is successful? Cas9 nuclease Cas1 Cas2 tracrRNA crRNA sgRNA donor DNAarrow_forwardBriefly describe how the CRISPR/Cas9 system modifies genomic DNA and why this can help treat genetic disease. Make it understandablearrow_forwardSequencing a genome and identifying individual genes are processes typically carried out - A. via manual transcription by a team of genetic scientists. B. by computers using high-throughput methods. C. by hand with a team of information scientists. D. using experimental processes.arrow_forward
- RNA transcripts are formed in the A. 3' to 5' direction by the RNA polymerase reading the DNA template in the 3' to 5' direction B. 5' to 3' direction by the RNA polymerase reading the DNA template in the 5' to 3' direction C. 5' to 3' direction by the RNA polymerase reading the DNA template in the 3' to 5' direction D. 3' to 5' direction by the RNA polymerase reading the DNA template in the 5' to 3' directionarrow_forwardSite-directed mutagenesis can be used to: a. treat diseases by gene therapy. b. make single residue mutation of a protein. c. make human insulin for treatment of diabetes. d. determine protein structures. e. determine DNA sequencingarrow_forwardFor the CRISPR/CAS system to function, DNA ligase must be added to the target cell. a. true b. falsearrow_forward
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