Which of the following can be a achieved by CRISPR/Cas9-based genome editing tool? A. Knocking out one or more gene B. Activating expression of a gene C. Suppressing expression of a gene D. All of the above

Q: Electrophoretic-Mobility Shift Assay DNA affinity chromatography Immunoprecipitation DNA footprint…

A: Introduction  DNA replication is the process by which a cell makes a copy of its genetic material,…

Q: organisms

A:

Q: which of these describes the symptoms of the disease(s) caused by mutations in this gene…

A: Mutation in the following gene i.e. XPA gene, causes malfunctioning of DNA repair mechanisms of our…

Q: One concern about using genetically-modified organisms is that many of the methods used to create…

A: CRISPR/Cas9 technology can be used to introduce targeted mutations or deletions in the genome by…

Q: Which of the following would restore a gene back to its proper reading frame? A. one insertion that…

A: A frameshift mutation is a genetic mutation caused by the insertion of deletion of a number of…

Q: Induced mutations

A:

Q: Which of the following statements about base editing is correct? 1. It involves nCas9. 2. The end…

A: Base editing  It is an alternative tool to HDR-mediated replacement. This facilitates editing of…

Q: Restriction enzymes (type II) bind to their recognition site and A. begin elongation from it B.…

A: Restriction endonucleases are those enzymes synthesized from bacterial cells that play an important…

Q: The function of a restriction enzyme is to a. prevent the movement of DNA outside the nucleus b.…

A: Restriction enzymes:- these are the proteins and mostly isolated from prokaryotes. These enzymes…

Q: The original function of CRISPR-Cas9 in bacteria is to: a. eliminate the function of any gene in…

A: CRISPR-Cas9 It refers to a gene editing tool that depends on the natural defense mechanism present…

Q: D 5. Choose the letter that corresponds to the step in the process where a restriction enzyme is…

A: The letter that corresponds to the step in the process where a restriction enzyme is used - Step A.…

Q: The enzymes mentioned below are used as tools during cloning, DNA sequencing and/or gene therapy.…

A: Enzymes  Enzymes are the protein that use as catalysts in metabolic and catabolic reaction to fasten…

Q: Suppose researchers learn that a particular congenital disease is caused by synthesis of a protein…

A: Recombinant DNA technology brings the the gene from multiple sources together and place it in the…

Q: Explain How Single-nucleotide mutations can be introduced into the genome using an engineered…

A: According to the question, we have to explain how single-nucleotide mutations can be introduced into…

Q: A collection of recombinant vectors that carry fragments of chromosomalDNA is calleda. a genomic…

A: Recombinant vectors are generally some live replicating virus particles which carry some extra genes…

Q: provide an argument/ what is the relevance of gene therapy in medicine using alziemer disease as an…

A: Gene therapy is a technique in which the gene is modified or edited that is some nucleotides can be…

Q: Discuss the process and give example on the following: a. Genome Project-write b. Mitochondrial…

A: The process of genome writing, mitochondrial DNA "spring cleaning," micro-encapsulation, and…

Q: Describe how the CRISPR-Cas system is used to modify genomes

A: Ans: CRISPR-Cas system which is the short form of clustered regularly interspaced short palindromic…

Q: The purpose of site-directed mutagenesis and CRISPR-Cas technologyis toa. determine if a protein…

A: Site-directed mutagenesis is a technique that produces mutation within a specific site in the cloned…

Q: I. List the sequences of RNA that would be transcribed from the following DNA template sequences. а.…

A: Transcription is the initial stage in gene expression, when genetic information is utilised to build…

Q: Transcription is the process of

A: Central dogma is proposed by Francis Crick. Central dogma states that the genetic information flows…

Q: Discuss the process and give example on the following. a. Aptamer Biosensors b. CRISPR c. Stem Cell…

A: A. Aptamers are single-stranded nucleic acids that selectively bind to target molecules. Most…

Q: If a scientist wanted to study the amino acid sequence of a protein, the best experimental approach…

A: Proteins are biopolymers that are made up of polypeptide chains of the monomeric units amino acids…

Q: What method allows researchers to make a large amount of human proteins in bacteria? Select one: a.…

A: The correct answer is option d. Gene cloning. Gene cloning is one of the methods that allow…

Q: Which of the following cannot usually be inserted into a vector to form a recombinant DNA?

A: a vector is a DNA molecule that is used as a vehicle to transfer foreign genetic material into a…

Q: CRISPR/Cas9 can be used in genome editing. Among the following statements, which one is correct?…

A: Bacteria have evolved various mechanism to resist against the viruses like bacteriophage some of…

Q: The ab initio approach finds genes by looking for a. common sequences found in most genes. b.…

A: Gene prediction is a computational biology process in which the encoding regions of a gene are…

Q: Which of the following steps is NOT involved in gene cloning? Lütfen birini seçin: O a. Transferring…

A: Gene cloning is the technique of isolating and forming copies of gene of interest.

Q: The goal of most gene therapies is to insert a healthy copy of a gene into the genome.  Besides…

A: The genome of an organism is defined as the whole genetic information that is inherited from one…

Q: The purpose of a ChIP-chip assay is to determinea. the expression levels of particular genes in the…

A: Introduction Genes are the particular sequence of nucleotides which gets expressed and give rise to…

Q: which of the following do researchers not need to use during vector cloning? a. a plasmid containing…

A: Certainly! Let's delve deeper into each option:a. Plasmid containing selectable marker genes: When…

Q: The polymerase chain reaction requires the following except A. primers complementary to the ends of…

A: Introduction: A specific DNA segment can be amplified in a laboratory setting using the Polymerase…

Q: The CRISPR system may be used to manipulate the genome in human cells, because Cas9 can

A: CRISPER system can use Cas protein to manipulate the genome present in human cell.

Q: Which of the following statements about coding strands of a gene is false? a. The sequence of the…

A: Coding strand is the DNA template whose nucleotide sequence is similar to the base sequence of the…

Q: Definition of Terms: a. Genetic Engineering b. DNA c. Recombinant DNA d. Plasmids e. Cloning…

A: A branch of biology that deals with the study of genes, genetic variation, and heredity in organisms…

Q: The function of CRISPR gene cluster in Streptococcus pyogenes is to Select one or more: O a. destroy…

A: CRISPR-Cas9 is a genome-editing mechanism that evolved organically in bacteria. CRISPR arrays are…

Q: Insertion of 3 base pairs at a random site in the genome can cause a frameshift.   A. Never B.…

A: The frameshift mutations are those which change the entire reading frame of the protein. Hence a…

Q: Which of these statements is correct regarding the CRISPR/Cas9 system in eukaryotic cells? A.Single…

A: Gene therapy has long been touted as a way to treat a wide range of human disorders and defects. The…

Q: Define the following terms:a. DNA typingb. short tandem repeatsc. DNA profiled. ribozymee. noncoding…

A: Introduction:

Q: Cuts the DNA into fragments Circular DNA molecule of bacteria  Used to insert DNA of interest to…

A: Restriction enzyme - When this enzyme identifies its target sequence, it will form a double-stranded…

Q: 8) PCR (polymerase chain reaction) 9) plasmid A. Creates millions of copies of DNA within hours B.…

A: PCR amplifies a specific region of a DNA strand (the DNA target). Most PCR methods amplify DNA…

Q: Consider the following human genetic diseases: hemophilia, Down syndrome, cystic fibrosis, and brain…

A: CRISPR(Clustered Regularly Interspaced Short Palindromic Repeats) Cas genome editing technology…

Q: DNA cloning refers to a. making cloned bacteria. b. making cloned sheep or other eukaryotic…

A: Sure! DNA cloning involves the creation of exact copies of a particular DNA fragment or sequence.…

Q: A bacteriophage strain carrying a point mutation is crossed with a bacteriophage strain carrying a…

A: The correct answer is option b. was outside the deletion region.  Here, a bacteriophage strain…

Q: CRISPR techniques allow scientists to modify specific genes while sparing all others, thus…

A: According to the question, we have to explain how CRISPR Technology helps to create better…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.