Which of the following can be a achieved by CRISPR/Cas9-based genome editing tool? A. Knocking out one or more gene B. Activating expression of a gene C. Suppressing expression of a gene D. All of the above

Consider the reagents required to perform CRISPR genome editing. Which molecule specifies the a new sequence that will occur at the target site if genome editing is successful? Cas9 nuclease Cas1 Cas2 tracrRNA crRNA sgRNA donor DNA

Briefly describe how the CRISPR/Cas9 system modifies genomic DNA and why this can help treat genetic disease. Make it understandable

Sequencing a genome and identifying individual genes are processes typically carried out  - A. via manual transcription by a team of genetic scientists. B. by computers using high-throughput methods. C. by hand with a team of information scientists. D. using experimental processes.

RNA transcripts are formed in the   A. 3' to 5' direction by the RNA polymerase reading the DNA template in the 3' to 5' direction   B. 5' to 3' direction by the RNA polymerase reading the DNA template in the 5' to 3' direction   C. 5' to 3' direction by the RNA polymerase reading the DNA template in the 3' to 5' direction   D. 3' to 5' direction by the RNA polymerase reading the DNA template in the 5' to 3' direction

Site-directed mutagenesis can be used to: a.   treat diseases by gene therapy.   b. make single residue mutation of a protein. c. make human insulin for treatment of diabetes. d. determine protein structures. e. determine DNA sequencing

For the  CRISPR/CAS system to function, DNA ligase must be added to the target cell. a. true b. false