CRISPR/Cas9 can be used in genome editing. Among the following statements, which one is correct?     The CRISPR and the Cas9 parts both recognise the DNA target sequence and then recruit an endonuclease for cutting it     The Cas9 part recognises the DNA target sequence and the CRISPR part cuts it     The CRISPR and the Cas9 parts both recognise and cut the DNA target sequence.     The CRISPR part recognises the DNA target sequence and Cas9 cuts it.

Briefly describe how the CRISPR/Cas9 system modifies genomic DNA and why this can help treat genetic disease. Make it understandable

The following is an example of what type of mutation? Normal DNA: 5' - ATGACGTCA - 3' Mutated DNA: 5' - ATGAGTCA - 3' Deletion Insertion Frameshift Silent

In the following "gene library" cloning experiment Digested genomic DNA AmpR gene TCR gene TCR is tetracycline resistant marker, AmpR is ampicillin resistant marker and BamHI is the unique restriction enzyme on plasmid. A PhD student digests/cuts the plasmids with BamHI restriction enzyme and the genomic DNA with EcoRI restriction enzyme. After performing the cloning experiment and obtaining colonies on a selection plate, the obtained cells will be ..... (Hint: this question is even more challenging; the PhD student was later demoted to an MSc student). a) resistant to ampicillin and tetracycline b) sensitive to tetracycline and ampicillin c) resistant to tetracycline and sensitive to ampicillin d) resistant to ampicillin and sensitive to tetracycline e) sensitive to ampicillin and tetracycline BamHI

If a researcher wanted to utilize CRISPR/Cas9 to target a gene of interest in their experimental system (i.e. Drosophila) which of the following would they customize for this purpose Group of answer choices Guiding region of sgRNA None of the above, CRISPR can only function in bacterial cells Cas9 enzyme Scaffold region of sgRNA

Two techniques used to make large amounts of a segment of DNA or gene of interest are: polymerase chain reaction and DNA cloning RNA interference and DNA sequencing polymerase chain reaction and DNA sequencing polymerase chain reaction and RNA interference  RNA interference and CRISPR

Which of the following are required to set up a CRISPR-Cas9 experiment to fix a mutation in a gene so that the gene sequence will be changed to the wild-type sequence? Circle all that apply. O CRISPR-Cas9 nuclease O sgRNA (single guide RNA) complementary to the part of the target sequence to be fixed O a PAM site (immediately adjacent to the target sequence in the genome) O donor DNA containing wild-type sequence of the part of the gene that is targeted (donor DNA can also be called repair template)

This is a multiple answers question. Which statement is true about CRISPR-Cas system? CRISPRS stand for clustered regularly interspaced short palindromic repeats Both bacteria and archaea can protect themselves from viral infection by using this system. Cas protein and short RNA form a complex The CRISPR region of DNA contains DNAS from previous infection by phages. Cas protein cuts target foreign DNA in pieces. The short RNA in the Cas protein and RNA complex has no function.

63.(2 correct choices)

If the researchers carry out a PCR for this gene from the CD8+ T cells, what result would you expect?Explain.