What is the geometry of molecules of the three dimensional shape of molecules in immunoassay methods ?
Q: What is Monoclonal Antibody? Write down its productions, applications, and limitations with proper…
A: Monoclonal antibodies are those antibodies that are identical to that of antibody which targets one…
Q: What is an introduction to ANTIBODY STRUCTURE AND FUNCTION ?
A: Antibody (Ab) or immunoglobin (Ig) is a Y-shaped protein produced by the immune system in response…
Q: What is indirect immunofluorescence microscopy?
A: BASIC INFORMATION MICROSCOPY It is a field in which the microscope used in order to objects which…
Q: What advantages do monoclonal antibodies have compared topolyclonal antibodies? How are mAbs…
A: Introduction Antibodies: Anti means “Against”; body here refer to antigens hence antibodies are the…
Q: What types of compounds in bacterial cells can serve as antigens?
A: Bacteria are prokaryotic cells, they are present in various shapes in nature like rod-shaped,…
Q: How would you measure deamidation in a monoclonal antibody?
A: A monoclonal antibody (mAb or moAb) is an antibody made up of a particular white blood cell. All…
Q: What is the difference between an antibody and an antibiotic?
A: Introduction Microorganisms are widely distributed and can be found in almost all type of…
Q: Explain what immunoaffinity extraction consists of when applied to obtaining steroids
A: Liquid chromatography (LC) and the targeted attachment of antibodies or related substances are both…
Q: What is the purpose of anti-human IgG antibodies in immunological testing?
A: Each IgG is composed of four peptide chains with two antigen binding sites - two γ-class heavy…
Q: What is the smallest unit of an antibody that can retain the specificity and binding affinity of the…
A: Antigen-binding fragments generated from the variable region of IgG and IgM include Fab2, Fab, Fab',…
Q: What are five commercially available immunodiagnostic kits?
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Q: Illustrate the solid phase “sandwich” immunoassaY AND What does the “s” in HBs stand for? What other…
A: Introduction: The antigen-antibody reaction is an association between an antigen and the antibody…
Q: Define Containment and explain their techniques.
A: The term containment refers to the safe methods or properly managing infectious agents in the…
Q: What are the important considerations that you have to remember in antibody screening?
A: Since you have asked multiple question, we will solve the first question for you. If you want any…
Q: Homogeneous Immunoassay (HIA) Application
A: Homogeneous ImmunoAssay: It is a biochemical test that evaluates the presence or concentration of a…
Q: What are the types of Immunomodulator agents? Please explain each of them? Please answer at your…
A: Immunomodulators are substances that operate on the pathways that control the immune system's…
Q: What is the name of protein subjected to IP? What is the importance of the pY blot? How many…
A: Immunoprecipitation or IP is a technology that precipitates a protein from the solution with the…
Q: Define the Immunotherapy with genetically engineered TCells ?
A: The treatment of diseases by activation or suppression of the immune system is immunotherapy or…
Q: hat Is Immuno Electrophoresis?
A: A complicated network of cells, tissues, organs, and the substances they produce that aids in the…
Q: What are natural activeimmunization and artificialactive immunization?
A: Immunity refers to every mechanism of the body that helps to protect the body from harmful foreign…
Q: How does plating efficiency affect the number of plaque-forming units? How is plating efficiency…
A: A viral plaque is defined as the visible structure formed on the culture plate of bacteria or any…
Q: What is the underlying principle behind the development of the COVID-19 antibody immunodiagnostic…
A: COVID-19 is the pandemic which is caused by the corona virus, it affects different individual…
Q: write down the following techniques of immunology and thier applications; 1.precipitation…
A: Since we only answer up to 3 subparts we'll answer the first 3. please resubmit the question and…
Q: Is co-immunoprecipitation from cells or tissues sufficient to prove that 2 GPCRs heterodimerize?
A: Dimerization of GPCRs initiates the signaling pathway by triggering an autophosphorylation in the…
Q: Which of these methods will allow visualization of protein localization in a cell? a. Flow Cytometry…
A: Protein localization is the technique which allows the visualization of a protein inside the cell.…
Q: What is rationale of using serological typing in bacterial agglutination?
A: INTRODUCTION Bacterial agglutination If a particular antigen such as bacteria is mixed with its…
Q: What are other methods of measuring the sensitivity of organisms to antibiotic?
A: An antibiotic is a substance that kills or inhibits the growth of bacteria and other microorganisms,…
Q: What is the principle of Immunochromatographic system, and its uses? Long anwser
A: Immunochromatography is the combination of immunoassay ( which is defined as biochemical tests that…
Q: Describe the two immunological diagnostic techniques that rely on a secondary antibody and explain…
A: Protein molecules known as antibodies or immunoglobulins are created by the body's immune cells.…
Q: Why are primary immunodeficiencies considered “experiments ofnature”?
A: Immunodeficiency is a state in which the immune system’s ability to fight infectious disease and…
Q: Discuss the structural and immunological basis of Ig isotype and subisotype according to Kuby…
A: Immunoglobulin G is the principle antibody in the serum it is also called Gamma globulin because of…
Q: What is the purpose of Deproteinization and why is it needed before chemical analysis of certain…
A: Proteins are a type of macro-nutrient (along with carbohydrates, fat and water) that are required by…
Q: What are steps in making an inactivated vaccine?
A: Vaccinations and Immunizations started in the 18th century, with the Smallpox Vaccination, created…
Q: What is the importance of Bacterial Vaccines?
A: Introduction : A biological preparation known as a vaccine offers active acquired immunity to a…
Q: In relation to immunotechnology, answer the following:Give ONE main difference between: Murine MABs…
A: MONOCLONAL ANTIBODY Monoclonal antibodies (MABs) are antigen-recognizing glycoproteins that are made…
Q: t Are The Uses Of Indirect Immuno Fluorescence?
A: The immunofluorescence technique, which used a fluorescence microscope to read the specific…
Q: What is the working principle of Lateral flowimmunochromatographic assays (LFIAs)? Supported with a…
A: We are giving answers only but we don't provide references. For a wide range of target analytes,…
Q: Explain how antibodies can be used as tools for protein identification, purification, and…
A: Introduction: Immunological techniques begin with the production of antibodies against a specific…
Q: which three items requires the use of Manual Antimicrobial Susceptibility Testing
A: Answer: Antimicrobial Susceptibility Testing : It is the manual testing for the drug used as an…
Q: explain or describe the principle of immunoturbidimetric C3 Assay.
A: Immunoturbidimetric: A technique that calculates the absorbance of light from an example which is…
Q: What are the main steps in an immunohistochemistry?
A: Immunohistochemistry is a process of immunostaining. In this process, the antigens are selectively…
Q: Describe three examples of how antibodies are used as reagents in cell biology and molecular…
A: An antibody is the soluble form of the B lymphocyte antigen receptor, and antibodies are made by…
Q: What is the principle of Immunochromatographic system, and its uses?
A: Immunology is the branch of science that deals with the study of immune systems and their…
Q: An enzyme-linked immunosorbent assay requires a catalytic antibody. a radioactive standard for…
A: The total protein content of a sample or a manufactured product must be understood through the…
Q: discuss the Kauffman-White antigenic scheme classification for Salmonella identification
A: The Kauffmann–White classification is a system that categorizes the Salmonella genus into serotypes…
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- Why do some tests for antibody in serum (such as for HIV and syphilis)require backup verification with additional tests at a later date?What is the working principle of Lateral flowimmunochromatographic assays (LFIAs)? Supported with a figure and reference plzOutline the principle behind the following techniques: 1. Immunofixation 2. Southern blotting 3. Ion exchange chromatograph 4. SDS-PAGE.
- Describe the process of immunohistochemistry to detect a specific protein of interest.Centrifugation is the first step in most fractionations, but it separates only components that differ greatly in size. Compare and contrast very high, high, medium, and low-speed centrifugations of supernatant mixtures. Define the following: SDS Polyacrylamide-Gel Electrophoresis and ImmunoprecipitationExplain how antibodies can be used as tools for protein identification, purification, and quantitation.
- How does a rapid test detecting RSV work and what kind of binding does it use (competitve, sandwich, indirect)? Explain using anti-rsv antibody conjugated with gold nanoparticle, anti-rsv antibody, and anti-human IgG. Draw a diagram if possible.c) In performing an immunological procedure, you add 2 mL of stock serum to 8 mL of saline. Two 1/4 serial dilutions are then made. What is the dilution of serum contained in the final tube? d) How would you perform a 1/5 dilution of glucose stock solution in a final volume of 1 mL (using water as a diluent)? Write all the steps of the procedure and show all your calculations.What does -log(p-value) tell in immunoprecipitation mass spectrometry? What does log2 Fold change say? Generally, what does the graph of immunoprecipitation mass spectrometry tell (ie. how to read its graph)?
- In the more common protocol for immunofluorescence detection of cellular proteins, an investigator uses two antibodies. The first binds specifically to the protein of interest. The second is labeled with fluorochromes for easy visualization, and it binds to the first antibody. In principle, one could simply label the first antibody and skip one step. Why use two successive antibodies?What is the immunoassay analyzer MachineAfter the electrophoretic separation, the proteins are transferred to a nitrocellulose membrane prior to staining with antibodies specific for the protein understudy. Describe in detail how the primary and secondary antibodies could aid in the protein detection process.