What are the important considerations that you have to remember in antibody screening?
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- What are the important considerations that you have to remember in antibody screening?
- What are the important considerations that you have to remember in antibody identification
-
What is the role of antibody screening in pretransfusion compatibility testing?
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- In general, what is the purpose of washing the red blood cell prior to testing? What are some of the tests that require washing of the red blood cells? What are the important considerations that you have to keep in mind in performing blood typing? Why is FORWARD typing only the screening procedure, not the confirmatory one? Why is REVERSE typing only the confirmatory procedure?A technologist failed to notice that the centrifuge had not properly centrifuged the test tubes prepared for antibody identification. The time of centrifugation was 15 seconds instead of 30 seconds. What would be the potential error in the interpretation of this test? can please any one provide me ans?What is the working principle of Lateral flowimmunochromatographic assays (LFIAs)? Supported with a figure and reference plz
- Why do some tests for antibody in serum (such as for HIV and syphilis)require backup verification with additional tests at a later date?1) Do you think eliminating the ultrasound is the best choice when it comes to the threat of a clot that could potentially break off at any point and travel to the heart or brain? 2) How effective do you think the pretest probability along with the D-Dimmer test would actually be?What does the acronym TPA stand for and how is TPA used in diagnostic medicine? Explain briefly.
- What are the two fluorescent compounds that can be used in antibody labelling?What are the advantages and disadvantages of agglutinationtests versus fluorescent antibody assays? How are the latter usedto identify specific cells in complex mixtures, such as blood?You just received the properly labeled blood bank specimen on patient Aran Stark. You decide to collect some background information about her known historical antibodies before beginning the work-up knowing that she has a history of anti-E, anti-K, anti-Jk^a, anti-Fy^a, anti-M and anti-Le^a. Which antibody can be neutralized? Which antibody is destroyed with 0.2M DTT treatment? Which antibody reactivity is enhanced by acidification? Which of the antibodies that are typically IgG in nature are destroyed by enzymes? Which are enhanced by enzymes? Which of these antibodies have been known to cause hemolytic transfusion reaction? Which of these antibodies are known to react at room temperature? Which of these antibodies react best at 37C? * When you complete the work-up, you note that the anti-Jk^a antibody is no longer detectable. Can the patient receive red blood cells that contain the Jk^a antigen? Why or why not?
- What is a screening test? Immunoassay Enzyme immunoassay (EAI) Enzyme-multiplied immunoassay technique (EMIT) Fluorescence polarization Radioimmunoassay (RIA) Chromatography Thin-layer chromatography (TLC) Gas chromatography (GC) Liquid chromatography (LC) (i.e, high performance liquid chromatography or HPLC) What is a confirmatory test? Hyphenated technique. Combination of two sophisticated technologies (I.e., Gas Chromatography - mass spectrometry or GC-MS) or other modern and acceptable techniques (l.e., LC-MS, GC-MS-MS, or LC-MS-MS).Three (3) true positives (antigen is present and patient's sera reacted) and three (3) true negatives (antigen is not present and patient's sera did not react): Question 3 options: Is a tool that can be used to increase the probability that any alloantibodies identified is/are correct Is an AABB requirement in order to prove each alloantibody specificity Proves that the antibody procedure was performed correctly Gives a P value of 95%ELISA tests usually use a primary and secondary antibody. Why? What are the necessary controls one would need to perform to ensure that the antibody specificities are valid (i.e., no false-positive or falsenegative reactions)?