How does a rapid test detecting RSV work and what kind of binding does it use (competitve, sandwich, indirect)? Explain using anti-rsv antibody conjugated with gold nanoparticle, anti-rsv antibody, and anti-human IgG. Draw a diagram if possible.

The substrate For the secondary antibody used in the lab for western blothing is :   TMB   TritonX-100   tween   BCIP/NBT

Our Western Blot experiment requires a buffer called TBS. At working concentration (1X), this buffer contains: 20 mM Tris 150 mM NaC1. Prior to use, however, the buffer is made and stored as a 10X concentrated stock What are the concentrations of these two ingredients in the 10X stock? Primary antibodies for the Western Blot experiment are prepared using this TBS buffer, with the addition of several other ingredients. Calculate the amount of each ingredient needed to produce10 ml of the final working antibody solution. b. 10X TBS stock c 0.01% (v/v) Tween detergent d. 3% (w/v) BSA (molecular weight 66430 g/mol) e. 1:10,000 dilution of antibody stock

1) SDS PAGE gels can be probed with NBT/BCIP to perform an immunoblot true/ false 2) the direction of migration of protiens through a matrix is due to most proteins in natur having a net negative charge  true/false 3)  PBST  is a buffer that is used for blocking the membrane during immunoblotting. true/false 4) the buffer used during for transferring proteins to nitrocellulose methanol. true/false 7) The size of the pores of a polyacrylamide gel dcreases as concentrations of polyacrylamide increases. True or false 10) The secondary antibody used in laboratory for western blotting is conjugated to which enzyme  A) Alkaline decarboxylase B) GAPDH C) Alkaline Phosphatase D) Horseeradish peroxide 13) In protien electrophroresis, what reagent present in the sample buffer is used to eliminate difference in the charge densities of protein A) SDS B) 2- mercaptoethanol C) Tris-HCL pH6.8 D) Tris- HCL PH 8.8

The substrate for the secondary antibody used in the lab for western blotting is:  triton X-100 BCIP/NBT Tween    TMB

Answer question in the photo

Why does the eliza with two antibodies incubate after adding the substrates?