How does a rapid test detecting RSV work and what kind of binding does it use (competitve, sandwich, indirect)? Explain using anti-rsv antibody conjugated with gold nanoparticle, anti-rsv antibody, and anti-human IgG. Draw a diagram if possible.

Q: Question 2 HER2 and HER3 belong to a family of receptor tyrosine kinases (RTKS). A representative…

A: The Human Epidermal Growth Factor Receptor (HER) or simply Epidermal Growth Factor Receptor (EGFR)…

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Q: How does Antibody test detect Covid-19 antibodies?

A: The glycoproteins which are synthesized by the B cells differing in their amino acid sequence and…

Q: identify the different cells in the blood smear image by their phenotypes and state their primary…

A: A. Lymphocyte Primary Function: responsible for immune responses and include B cells, T cells, and…

Q: Why do you coat the elisa plate with albumin?

A: ELISA It is enzyme linked immunosorbent assay. It is a common and powerful technique used for…

Q: If the protein produced full length antibody what would be the next step after centrifugation?

A: Antibodies are protein related to immune system know as Immunoglobulin. Each antibody is made up of…

Q: Would it be worse for the function of the antibody for it to lose the Fc region or a Fab region?…

A: The  N-terminal fragment square degree is called the beautiful space, and also the C-terminal…

Q: particles can heterophile antibody agglutinate

A: A test used to detect quickly the infectious mononucleosis (IM) also known as glandular fever which…

Q: Why is a blocking buffer needed while running the immunoblotting of the PDVF membrane, furthermore…

A: Immunoblotting, like ELISA, uses host antibodies to identify specific parasite antigens. Protein…

Q: In the SARS-CoV-2 assay, what is the benefit of a diagnostic tool that can distinguish the…

A: The novel Coronavirus infection SARS CoV 2 can be diagnosed by different means. Proper diagnosis,…

Q: Describe the principles behind direct and indirect fl uorescent antibody tests.

A: Fluorescent antibody technique is a laboratory technique in which the labelled antibodies bind to…

Q: Why does the antibody titer determination use twofold dilutions ofthe antiserum rather than 10-fold…

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Q: Write down basic principle of ELISA and its main steps? Write names of different types of ELISA

A: Enzyme linked immunosorbent assay abbreviated as ELISA . A plate based assay  technique used to…

Q: EIAs are extremely sensitive diagnostic tools. Why,then, is the immunoblot (Western blot) procedure…

A: EIA stands for enzyme immunoassay, is a diagnostic test that can be divided into two types based on…

Q: 1.)the order of steps done for immunoprecipitation is a.) 1° Ab, 2° Ab, SDS-PAGE b.)SDS-PAGE, 1 °Ab,…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Briefly describe how viral antigens can be screened for using an enzyme-linked immunosorbent assay…

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Q: What is a recombinant DNA vaccine? List two such vaccines. State their advantages.

A: A vaccine is a preparation of killed or weakened or  a part of microbe (antigen) that is used to…

Q: What are some ways that the antibody-protein complex can be visualized in ELISA or western blots?

A: Introduction: The theory of antibody/antigen binding is used in ELISA assays. They make it possible…

Q: There are many immunotherapeutics now being used to immunomodulate recipients that are either fully…

A: Wellbeing and immuno-toxicity appraisal of immunomodulatory monoclonal antibodies Most remedial…

Q: Name two types of immunodiffusion tests and describe how they operate

A: Immunodiffusion is a test which involves the use of soft agar or agarose to allow diffusion of…

Q: if you are running quantitative test where serum is diluted 6 times and you observed agglutination…

A: Agglutination is the mechanism by which antibodies pile together all the cells or particles like…

Q: How would a lateral flow immunoassay using breast milk to test high levels of concentrations of IgG…

A: Introduction:- The Transplacental of the transfer of the RSV immunoglobulin G (IgG) antibodies via…

Q: If you Infect the Jurkat T lymphocyte cell line with HIV-1 strain IIIB. Lyse the infected cells at…

A: A virus is a microscopic infectious agent that could infect and live only within the living…

Q: For an Immunoprecipitation experiment:The cellular extract contains a protein labeled with a…

A: Immunoprecipitation is a technique used to isolate or detect the presence of a specific protein…

Q: Write down basic principle of ELISA and its main steps? Write names of different types of ELISA

A: Introduction: ELISA(enzyme-linked immunosorbent assay) is a technique used to detect specific…

Q: Cell line A was cultured in the absence and presence of KB9520 or PPT. You then lyse the cells and…

A: Estrogen receptors (ERs) are a family of transcription factors that play a critical role in a…

Q: For Western Blot detection of specific proteins, the secondary antibody directly binds to: A) The…

A: An antibody is a protein molecule generated in the living body by plasma membrane of B-cells, which…

Q: Is rapid antibody testing a form of ELISA? Explain your answer. How is an ELISA different from rapid…

A: The objective of the question is to understand if rapid antibody testing is a form of ELISA…

Q: why would there be some additional bands on an immunoblot that do not match the molecular weight of…

A: Immunoblot or western blot is a technique where we can detect the presence of a particular protein…

Q: 1.What does the “s” in HBs stand for? What other antigens are present in HB? 2. The presence of IgM…

A: Hepatitis is a systematic disease that causes the liver immflamantation which could be viruses,…

Q: Four mice (M1, M2, M3 and M4) were injected with the recombinant S1 protein alone and four mice (M5,…

A: ELISA (Enzyme-Linked Immunosorbent Assay) is a widely used laboratory technique that allows the…

Q: A patient is O positive and his serum is agglutinating all screening and panel reagent cells at room…

A: Blood group of the given patient is O positive. His serum is agglutinating all screening and panel…

Q: What are steps in making an inactivated vaccine?

A: Vaccinations and Immunizations started in the 18th century, with the Smallpox Vaccination, created…

Q: How is signal amplification achieved in enzyme-linked immunosorbent assays?

A: Enzyme-linked immunosorbent assays or ELISA refers to the techniques to detect antibodies, hormones,…

Q: 2. 3. The following antibody screen was performed on a 65 year old male who was hospitalized for a…

A: Introduction "An antibody screen is a laboratory test that is used to detect the presence of…

Q: Compare the advantages and disadvantages of EIA, rapid tests,and immunoblots with respect to speed,…

A: The immune system is a complex network of biological structures (cell) and protein that defends the…

Immunology

Immunology is the division of science involved with the immune reactions occurring in living organisms. The organisms are accustomed to evolving internal defense mechanisms to evade the invasion of microbial pathogens. Diverse cells are connected with stimulating the immune responses, which produce inhospitable conditions to the microbes prompting their lysis and thus inhibits the multiplication of the pathogens. The immune system can distinguish the pathogens through the aid of cellular intercommunication and launch responses to destroy them.