Q: What is indirect immunofluorescence microscopy?
A: BASIC INFORMATION MICROSCOPY It is a field in which the microscope used in order to objects which…
Q: What is immunoassay? Differentiate direct and indirect immunoasay tests.
A: In immunology, antigen (Ag) refers to any particle or object that is capable of elucidating immune…
Q: What types of compounds in bacterial cells can serve as antigens?
A: Bacteria are prokaryotic cells, they are present in various shapes in nature like rod-shaped,…
Q: What is a vaccine?
A: Vaccines are a part of artificial active immunity. Antigens or a mixture of antigens which are used…
Q: Which ethical issue is solved by induced pluripotent stem cells? Mention one advantage of using…
A: Induced pluripotent stem cells (iPSCs) are Artificial stem cells that are delivered from somatic…
Q: If the protein produced full length antibody what would be the next step after centrifugation?
A: Antibodies are protein related to immune system know as Immunoglobulin. Each antibody is made up of…
Q: Antigen Antibody Enzyme-labelled antibody Substrate This image represents a/an. direc ELISA sandwich…
A: Enzyme-linked immunosorbent assay (ELISA) is an immunological assay that is used in laboratories to…
Q: What do you mean by immunofluorescence
A: Antibodies are immunoglobulins produced in response to antigenic stimulation. They may either bound…
Q: What is the cancer cell line name used in hybridoma technology?
A: Hybridoma technology may be a technique for manufacturing massive numbers of identical antibodies.…
Q: Which tests would be faster to perform? PCR or an ELISA test?
A: ELISA ELISA is enzyme linked immunosorbent assay. It is based upon antibody-antigen interaction.…
Q: What is the purpose and importance of the Immunology-Serology section in the laboratory?
A: Serum is the fluid and solute component of blood which does not play a role in clotting.
Q: Describe the process of immunohistochemistry to detect a specific protein of interest.
A: or ImmunoHistoChemistry ( IHC), is an uncommon staining measure performed on new or frozen breast…
Q: Why is a blocking buffer needed while running the immunoblotting of the PDVF membrane, furthermore…
A: Immunoblotting, like ELISA, uses host antibodies to identify specific parasite antigens. Protein…
Q: Explain the statistical findings regarding the lateral flowimmunochromatographic assays sensitivity…
A: The lateral flow test is one of a simpler immunochromatographic method that confirms the absence or…
Q: What is the principle of Immunochromatographic system, and its uses? Long answer please
A: Answer: CHROMATOGRAPHY TECHNIQUE = It is the technique which is used to separate the components of a…
Q: Homogeneous Immunoassay (HIA) Application
A: Homogeneous ImmunoAssay: It is a biochemical test that evaluates the presence or concentration of a…
Q: Why does the antibody titer determination use twofold dilutions ofthe antiserum rather than 10-fold…
A:
Q: What is the DNA vaccine?
A: Immune system is system which helps our body to fight against the foreign substances which will…
Q: hat Is Immuno Electrophoresis?
A: A complicated network of cells, tissues, organs, and the substances they produce that aids in the…
Q: What are the advantages/disadvantages of using a direct immunoblotting method? Compare and contrast…
A: Immunoblotting (western blotting) techniques are a rapid and highly sensitive method for the…
Q: What component must have been present in the positive control solution (antigen, anti-HIV IgG…
A: Enzyme-linked immunosorbent assay (ELISA) is a plate-based assay technique designed for detecting…
Q: explain the Immunofluorescence Staining Protocol
A: Immunofluorescence is a method of visualization of a specific protein or antigen in cells or tissues…
Q: Based on the given picture, answer the following questions (5 ,6 and 7). How will you describe…
A: Immunodiffusion is the process in which antigen precipitates with an antibody inside an agar gel as…
Q: Which of these methods will allow visualization of protein localization in a cell? a. Flow Cytometry…
A: Protein localization is the technique which allows the visualization of a protein inside the cell.…
Q: why do we have to do a western blot confirmatory testing after doing an ELISA screening test for…
A: The human immunodeficiency virus causes the chronic virus infection known as acquired…
Q: Name two types of immunodiffusion tests and describe how they operate
A: Immunodiffusion is a test which involves the use of soft agar or agarose to allow diffusion of…
Q: how does immunoessay technic shows the Bence-Jones protein in the urine for the diagnosis of the…
A: Bence jones's proteins are composed of dimers of light chain immunoglobulins S and due to their…
Q: What is the principle of Immunochromatographic system, and its uses? Long anwser
A: Immunochromatography is the combination of immunoassay ( which is defined as biochemical tests that…
Q: What do you mean by the term "Antibiotic resistance" ? Use your own words to explain
A: Antibiotics are the chemical substances that used to stop or destroy the activity of various micro…
Q: Which technique is best for determing the the intracellular localization of a protein? Question…
A: Intracellular localisation of protein is the determination of the cellular components where protein…
Q: What is the geometry of molecules of the three dimensional shape of molecules in immunoassay methods…
A: Shape is an important molecular feature determining the fate of a compound. It does so in terms of…
Q: Discuss the process and give example on the following below: a. DNA Microarray Technology b.…
A: Different cell therapies are utilized in the laboratories to produce various important processes…
Q: What are steps in making an inactivated vaccine?
A: Vaccinations and Immunizations started in the 18th century, with the Smallpox Vaccination, created…
Q: Using flow diagrams show the main steps in antigen and antibody detection ELISA's.
A:
Q: Homogeneous Immunoassay (HIA) Principal
A: An immunoassay is a biochemical test which measures the concentration of macromolecules or small…
Q: The process where antibody will cause microbes to be connected together so they can no longer cause…
A: When foreign molecules attack the body, protective molecules are produced by our immune system to…
Q: What is the importance of Bacterial Vaccines?
A: Introduction : A biological preparation known as a vaccine offers active acquired immunity to a…
Q: Why do vaccine manufacturers begin with tests on animals or cell lines before moving on to adult…
A: Vaccines are the antigens that are administered into the body.
Q: What is the process in which antibodies attach to antigens, causing the formation of masses of…
A: Because the Y-shaped antibody arms randomly attach to many surfaces of non-self red blood cells,…
Q: which three items requires the use of Manual Antimicrobial Susceptibility Testing
A: Answer: Antimicrobial Susceptibility Testing : It is the manual testing for the drug used as an…
Q: Write the introduction of antibodies in biotechnology and write a short history of antibodies
A: Definition of antibodies: Antibodies are proteins that can attach to particular substances known as…
Q: What is unique about the use of viral gene therapy in cancer immunotherapy
A: Virus have natural ability of delivering genetic material into the cells. Therefore, some of the…
Q: How reliable are Lateral flowimmunochromatographic assays when compared to molecular diagnostic…
A: COVID-19 is a respiratory disorder caused by a novel coronavirus (SARS-CoV-2). The genetic…
Q: Viral enzyme analysis Infectivity analysis
A: Viruses are simple, noncellular entities consisting of one or more molecules of either DNA or RNA…
Q: In your own words, explain why this procedure is referred to as an enzyme-linked immunosorbent…
A: ELISA is the most widely used test in serological diagnosis. It is well used for the detection of…
Q: An enzyme-linked immunosorbent assay requires a catalytic antibody. a radioactive standard for…
A: The total protein content of a sample or a manufactured product must be understood through the…
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- What is the principle of Immunochromatographic system, and its uses? Long answer pleaseIn this chapter, we described co-immunoprecipitation as a method for identifying binding partners to a protein of interest. A simpler variation of this method can also be used to isolate proteins of low abundance in a complex mixture. Arrange the steps in sequential order to use this technique for this purpose. First step Last step Answer Bank The protein mixture is centrifuged, the supernatant is removed, and the pellet is washed. The antibody binds to the protein of interest. The antibody-protein complexes become insoluble. An antibody against the protein of interest is added to the mixture. Protein A beads are added to the mixture.What is an introduction to ANTIBODY STRUCTURE AND FUNCTION ? How would you summarize this to someone who is new to this? https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2726606/
- Can S-layer proteins be detected by immunolabelling when a capsule is present? How do you know? I need help finding the answer in the article and explain in short answer link to article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/What Is Immuno Electrophoresis?What is the geometry of molecules of the three dimensional shape of molecules in immunoassay methods ?
- For an Immunoprecipitation experiment: What is the function of the IP-2 tube in this experiment?What is the importance of incubating the ELISA plate?To diagnosis, we used a procedure known as a "sandwich ELISA" (Enzyme-Linked Immunosorbent Assay). 1.In the "ELISA sandwich," what is the "meat"? 2.Both patients A and B had positive results. Why are these two patients' outcomes so different? 3.What is one potential cause of an ELISA false negative result? What is one potential cause of an ELISA false positive result? What is another example of how an ELISA assay should be used?
- Why do some tests for antibody in serum (such as for HIV and syphilis)require backup verification with additional tests at a later date?What is the purpose of anti-human IgG antibodies in immunological testing?A technologist failed to notice that the centrifuge had not properly centrifuged the test tubes prepared for antibody identification. The time of centrifugation was 15 seconds instead of 30 seconds. What would be the potential error in the interpretation of this test? can please any one provide me ans?