What is the effect of moist heat and dry heat? Define TDT and TDP. What is the minimum sterilization condition of autoclave? What is tyndallization, incineration, pasteurization.
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What is the effect of moist heat and dry heat? Define TDT and TDP. What is the minimum sterilization condition of autoclave? What is tyndallization, incineration, pasteurization.
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- What is the point of the pressure in the autoclave sterilization?What settings and time are normally used for sterilization in an autoclave?In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropper
- Other than the application of heat, name other methods of sterilization. Define the methods and give examples of materials sterilized in each method.Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? What precautions should be taken to control laboratory contaminants? Of what advantage is the using a solid and a liquid medium?Is the autoclaving process absolute in killing all microorganisms? If not, what are the factors that hinder effective sterilization through autoclaving? When can you say that the sterility of an instrument is compromise?
- Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Of what advantage is the using a solid and a liquid medium?What type of media components cannot be sterilized in an autoclave?Why is the time of exposure and temperature required for sterilization greater for dry heat than for autoclaving?
- Is the autoclaving process absolute in killing all microorganisms? If not, what are the factors that hinder effective sterilization through autoclaving?List 5 other methods of sterilization and describe the principle/s and when each method is used. What are the requirements to support microbial growth?Which of the following is a limitation of the autoclave? a) Long length of time required for sterilization. b) Inability to use with heat sensitive materials. c) Inability to use with common lab equipment like glassware. d) Does not sterilize, only sanitizes. e) All of the above are limitation of the autoclave.