Q: What advantages do solid media offer for the isolation ofmicroorganisms?
A: Micro-organism are those that cannot be seen by the naked eye, but are visible under microscopes.…
Q: Oven sterilization at 121˚C was performed on glassware for 15 minutes only. Effect: Rationale:
A: Sterilization It is defined as the process in which micro-organisms whether bacteria, fungi,…
Q: Explain the purpose of thiosulfate in the medium.
A: Culture media is used to enrich the number of bacterial growth, to select certain bacteria and…
Q: What is sterilization? Give specific examples of media which are sterilized by the following…
A: All microbes, such as bacteria, virus, and fungi, requires some essential nutrients for growth. The…
Q: Can culture media be sterilized in the hot air sterilizer? Why or why not?
A: Hot air sterilization is a procedure in which the sterilization of the objects are done using hot…
Q: What nutrients do the following media components contain? • Peptone Yeast extract Beef extract •…
A: Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: Why is resazurin a useful media additive for the study of anaerobes?
A: Resazurin is a weakly fluorescent, non-toxic, redox-sensitive phenoxazine dye which has a…
Q: Define sterilization.
A: Microbial control indicates the prevention or inhibition of microbial growth. It involves physical…
Q: which of the following is best to sterilize heat-liable (sensitive) solutions? a, pasteurization…
A: From above question Option d (filtration) is correct.
Q: Agar is a solidifying agent used in media preparation. What is its origin?
A: Agar gel is a polysaccharide which can become liquefied at temperatures ranges from 85°C to 90°C and…
Q: In the laboratory, autoclaving is required to prepare media for the growth of What type of method of…
A: The most effective method by which we sterilize the laboratory instruments is known as autoclaving.
Q: Why does the commercial production of yeast require special equipment to achieve adequate aeration,…
A: High yield of yeast cannot be attained unless air in a huge quantity is injected in fermentation…
Q: what type of bacteria would be used to make sauerkraut?
A: Sauerkraut is product of cutting raw cabbage that fermented by various lactic acid bacteria. The…
Q: What concentration of agar is used in a semisolid medium for motility determination? How does that…
A: Agar is a polysaccharide that is obtained from marine seaweeds. Agar is mostly used in food and…
Q: What is the difference between defined media and complex media?
A: media is a solution in which is rich in nutrients. it is very suitable for the growth of microbes…
Q: Why are complex media preferable to chemically defined media for routinecultivation of…
A: Microorganisms require nutrients as an energy source and certain other environmental conditions to…
Q: Why would hydrogen peroxide be an effective disinfectant?
A: We have to answer why hydrogen peroxide can be an effective disinfectant.
Q: Why is the batch bioreactor is suitable in milk fermentation?
A: Batch bioreactor is a closed system and the process lasts until the nutrients are consumed.
Q: What is differential medium and differential media? Explain the difference ?
A: Difference between differential medumium - .- A differential medium support the expansion of any…
Q: What is the correlation between radiation “energy” and the mechanisms of sterilization?
A: Safety is of utmost importance while working with any contagious or pathogenic substance or living…
Q: Nitrate reduction to nitrite is indicated by the formation of a pink to red colour after addition of…
A: Sulfanilic acid reacts with nitrous acid to form diazotized sulfanilic acid if introduced. This…
Q: Why is PBS buffer preferred when preparing a monolayer cell culture?
A: PBS (phosphate buffer saline) is a buffer solution which is a water based salt solution containing…
Q: What is the use of hay infusion in determination of microbial motility?
A: Hay infusion use in determination of microbial motility.
Q: What settings and time are normally used for sterilization in an autoclave?
A: The fundamental rule of steam sterilisation as cultivated in an autoclave, is to open every thing to…
Q: Explain why it is essential that bacteria cultures be sterilized in an autoclave.
A: The microbial cultures are grown in the laboratory so as to multiply them or increase the number of…
Q: placing bacteria at ultracold temperatures is as effective at killing microbes as the use of dry…
A: true
Q: What is the name of this media? What type of media is it? What does the yellow color indicate? What…
A: Selective media generally selects for the growth of a desired organism, stopping the growth of or…
Q: For the Fluid Thioglycollate Medium, draw the five possible outcomes in the sections below and label…
A: Disclaimer: According to the Bartleby guidelines only a single question can address at once. Hence,…
Q: Why is 15 minutes generally used for sterilization? Why is agar added to medium?
A: Sterilization is the process in which all life forms like microbes (fungi, bacteria, viruses,…
Q: In an autoclave, how are temperatures above boiling achieved? (Answer in not more than 2 sentences)
A: The air in the autoclave chamber is removed and replaced by the increasing amount of steam trapped…
Q: Requirements of Autoclave for sterilization
A: autoclave is an instrument used for sterilization of equipments and media cultures etc in the…
Q: Why does hydrogen peroxide are most effective against probiotics?
A: Hydrogen peroxide is a peroxide. It acts as an oxidizing agent
Q: Why is the temperature of pasteurization at 60°C?
A: Pasteurization was invented by a French Scientist called Louis Pasteur. It was first used in 1864.…
Q: In the tripple sugar fermentation test, why is the triple sugar iron agar called a multi-test…
A: The process of chemical breakdown of complex substances into simple ones in the absence of oxygen…
Q: Why are plated media stored in an inverted position?
A: Plated media enables the isolation of microorganisms from samples or specimens.
Q: What conditions are necessary to destroy endospores? In what device are these conditions achieved?
A: Endospores are dormant vegetative cells that have a thick outer covering making them resistant to…
Q: What is the meaning of the word sterile? What would happen if culture media freshly prepared were…
A: Sterile It refers to the state of being free from any bacteria or any other living microorganisms.…
Q: What method can be used to achieve sterilization?
A: It is required to describe the method to be used to achieve sterilization.
What type of media components cannot be sterilized in an autoclave?
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- What settings and time are normally used for sterilization in an autoclave?Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Of what advantage is the using a solid and a liquid medium?Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? What precautions should be taken to control laboratory contaminants? Of what advantage is the using a solid and a liquid medium?
- In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperWhat is the difference between defined media and complex media?Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Why are petri dishes incubated in an inverted position? Of what advantage is the using a solid and a liquid medium?