What is sterilization? Give specific examples of media which are sterilized by the following methods: A. Boiling or heating only B. Autoclave using steam under pressure. C. Inspissator

Q: Isolating a pure culture from a mix sample is typically done on solid agar but can be performed…

A: A microbial culture is a method of multiplying microbial organisms by letting them reproduce in…

Q: What is the difference between Dry and Wet Celloidin Method?

A: Celloidin method is a histopathological technique of tissue impregnation and embedding that helps to…

Q: Explain why a green metallic sheen is formed when E. coli is grown on Eosin Methylene Blue Agar.

A: Escherichia coli is the gram negative bacteria.

Q: Why should the use of nichrome or iron containing loop/wire be avoided as an inoculating device when…

A: The oxidase is a test used in microbiology to identify bacteria that produce cytochrome c oxidase.…

Q: Which of the following would be best for sterilizing a pre-sealed case of plastic medical supplies?

A: Answer - Autoclave

Q: Why is resazurin a useful media additive for the study of anaerobes?

A: Resazurin is a weakly fluorescent, non-toxic, redox-sensitive phenoxazine dye which has a…

Q: Agar is a solidifying agent used in media preparation. What is its origin?

A: Agar gel is a polysaccharide which can become liquefied at temperatures ranges from 85°C to 90°C and…

Q: What is the effect of moist heat and dry heat? Define TDT and TDP. What is the minimum sterilization…

A: Sterilization is defined as a technique which is used for complete removal, killing or deactivation…

Q: how do microorganisms such as bacteria eliminate harmful reactive oxygen species?

A: Reactive oxygen species (ROS) are emerging as important elements in the bacterial response to lethal…

Q: Explain about different types of bioreactors and how they are used in cell suspension culture. What…

A: Ans: Bioreactor It refers to any device that supports the biological environment. This is used for…

Q: Why is immobilization a good strategy for dealing with uraniumpollution?

A: Uranium (U) is a radioactive chemical element of the actinoid series of the periodic number with an…

Q: What is the difference between defined media and complex media?

A: media is a solution in which is rich in nutrients.  it is very suitable for the growth of microbes…

Q: Which of the following statements is true regarding steam sterilization? a. It is a universal…

A: steam sterilization , also called autoclave sterilization , is used in microbiological laboratory in…

Q: Under what conditions are media typically sterilized in an autoclave?

A: An autoclave is the pressure chamber used to carry out the scientific and industrial processes…

Q: What is differential medium and differential media? Explain the difference ?

A: Difference between differential medumium -   .- A differential medium support the expansion of any…

Q: Why is PBS buffer preferred when preparing a monolayer cell culture?

A: PBS (phosphate buffer saline) is a buffer solution which is a water based salt solution containing…

Q: Compare and contrast use dilution and disk diffusion methods of evaluating disinfectants. Please…

A: Chemical disinfectants are gathered by the sorts of organisms and infectious agents they are…

Q: What are the different types of disinfectants? How is each one different or similar from the others?…

A: Almost every environment on the planet contains bacteria and microorganisms. In fact, on one square…

Q: What main advantage do microbial plastics have over syntheticplastics?

A: Plastics are organic materials. It is derived from the Greek word “plastikos”.

Q: The most probably number (MPN) method for measuring Nitrifiers in soil Microbiology ?

A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…

Q: Describe and give the principle of autoclave, Hot Air Oven and a Hot Plate used for sterilization in…

A: Sterilization in microbiology: This technique is used for the killing of the harmful bacteria on the…

Q: What are some advantages and disadvantages of using ionizing radiation to sterilize food?

A: Sterilization involves physical methods for removing or controlling the microbes. The physical…

Q: What settings and time are normally used for sterilization in an autoclave?

A: The fundamental rule of steam sterilisation as cultivated in an autoclave, is to open every thing to…

Q: Explain why it is essential that bacteria cultures be sterilized in an autoclave.

A: The microbial cultures are grown in the laboratory so as to multiply them or increase the number of…

Q: Why are most antimicrobial chemical agents disinfectants rather than sterilants? What general…

A: Most chemical antimicrobial agents are disinfectants instead of sterilants. A disinfectant reduces…

Q: What are the possible ways to sterilize heat labile products? Explain your answer with suitable…

A: Heat labile materials are those substances that can undergo change rapid change when exposed to high…

Q: How does an autoclave sterilize instruments? What is used to dry glassware and at the same time

A: Autoclaving is the best method for sterilizing glass instruments. Sterilizing means removal of all…

Q: What is the point of the pressure in the autoclave sterilization?

A: Autoclaves are the devices which are like pressure cookers and  used in laboratories that provide a…

Q: what is the difference between volatile oils and fixed oils in terms of degradation?

A: Oil is a triglyceride that is liquid at room temperature. It is a nonpolar chemical substance that…

Q: What are the advantages of solid media  ?

A: Microbial culture is a method of multiplying microbes by allowing them to reproduce in a…

Q: Requirements of Autoclave for sterilization

A: autoclave is an instrument used for sterilization of equipments and media cultures etc in the…

Q: What is diluent? Why is it useful for working with bacterial samples?

A: Dilution is done to decrease the concentration of the original sample that is being tested. Dilution…

Q: ther than the application of heat, name other methods of sterilization. Define the methods and give…

A: Sterilization is the process of completely destroying all microorganisms on the surface of an…

Q: Many culture media produce colorful solutions due to the presence of which are water soluble organic…

A: Colony color or pigmentation is mostly exploited characteristics of bacteria which aids in the…

Q: Why is the sterilization important in healthcare system and can you give briefly explain?

A: Medical sterilization mainly makes use of three important terms such as sterilization, disinfection…

Q: Which of the following items is the least suitable for sterilization by autoclave? a. Water for…

A: An autoclave is a machine that is used to perform industrial and scientific processes that require…

Q: What is saponification? How can we make soap? What is the reaction involve in making soaps? Whats…

A: Soaps and detergents are commonly used in cleaning clothes, utensils and other dirty surfaces.…

Q: What is the hazard of the splattering tendency in flame sterilizing an inoculating loop? Can…

A: Aseptic preparation of culture media :-

Q: When you did Wright-Giemsa staining, you added the blue dye in the well containing C2C12 cells. The…

A: Lyophilization is a process of freeze-drying the sample such as cells and tissues under a vacuum…