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What are the common causes of failed genomic DNA extraction in bacterial cells?
Step by step
Solved in 2 steps
- What is the mechanism by which these designed nucleases cause a change in the genomic DNA sequence?It is desired to isolate genomic DNA from liquid culture of S. cerevisiae yeast. A commercial kit will be used to isolate genomic DNA from this liquid culture. Answer the following questions to understand the strategy used by commercial kits for genomic DNA isolation. a) List all the steps from cell pellet preparation to DNA elution. b) With which feature can the membrane in the column that comes with the commercial kit bind DNA? c) Which component in the kit would you use to recover the DNA from the membrane of the column to which the DNA was attached?What are the possible advantages or disadvantages to the organism or its offspring resulting from DNA modification by environmental factors?
- What are the DNA extraction methods? Explain each one.Xeroderma pigmentosum is a genetic disease caused by an error in the nucleotide excision repair process that fixes damage to DNA by ultraviolet light. Studies have shown that it can result from mutations in any one of seven genes. What can you infer from this finding? A) There are seven genes that produce the same protein B) These seven genes are the most easily damaged by ultraviolet light. C) There are seven enzymes involved in the nucleotide excision repair process. D) These mutations have resulted from translocation of gene segments.Define and compare the outcomes of the following types of nucleotide substitutions, insertion or deletions. Which is likely to cause the least dramatic mutant effect? a) missense mutations b) nonsense mutations c) frameshift mutations d) silent mutation
- Unlike eukaryotic organisms, bacteria can recombine their genome with foreign DNA.What are the methods bacteria use to recombine their DNA?What is the application of DNA extraction in healthcare? Cite at least 3 specific examples.What is the purpose of DNA methylation? Why is it ineffective in the DNA repair of spontaneous deamination of C? Explain in 4 to 6 sentences.