What assay uses the fact that activators can be separated into DNA binding and activating domains? What information do you gain from this assay? Describe briefly how to perform this assay.

What is the role of streptomycin in CRISPR experiment? What biochemical changes (DNA, protein) occurred in those cells in which CRISPR worked?

Describe the steps of the chromatin immunoprecipitation assay.   What are some of the scientific facts that can be learned through a chromatin immunoprecipitation assay?

Briefly describe the method of chromatin immunoprecipitation sequencing (ChIP-Seq). How is it used to determine nucleosome positions within a genome?

CCCP is observed to have caused increase the SDH activity in DCIP assay. Explain why?

Can you give an example of a modified (tagged) primers to amplify E. coli UidA sequence and subclone into appropriate vector (pUC19)?

What factors could explain a transformation efficiency?

Will restriction sites for an enzyme that has 4 bp in its recognition sequence be closer together, farther apart, or similarly spaced, on average, compared with those of an enzyme that has 6 bp in its recognition sequence? Expalin your reasoning?

Select all that apply from the following to each transformation process : The following are White non-glowing colonies White glowing colonies Blue non-glowing colonies Blue glowing colonies None of the above The transformation are: a. LB + kanamycin plates that have been spread with the E. coli cells transformed with your ligations, what phenotype of the colonies do you expect to obtain . b. LB + kanamycin plates that have been spread with the E. coli cells transformed with water, what phenotype of the colonies do you expect to obtain. c. LB plates that have been spread with the E. coli cells transformed with your pHSG298, what phenotype of the colonies do you expect to obtain. d. LB + kanamycin plates that have been spread with the E. coli cells transformed with PHSG298, what phenotype of the colonies do you expect to obtain. e. LB plates that have been spread with the E. coli cells transformed with your ligations, what phenotype of the colonies do you expect to obtain. f. LB plates…

Please offer a clear explanation of the ChIP assay.  A. What characteristics of these cells are being assessed? Why would this be valuable to a research team? B. Define assay and explain how samples are managed step by step with a clear explanation of each step. C. What type of data is being generated by this experiment?