What assay uses the fact that activators can be separated into DNA binding and activating domains? What information do you gain from this assay? Describe briefly how to perform this assay.

Q: You observe the following: Your Your friend's Gel Gel 120 kDa 100 kDa 120 kDa 100 kDa -80 kDa 80 kDa…

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Q: Could you explain how you would know if you successfully induced transformation? Thank you Did you…

A: Transformation experiments are very crucial for understanding fundamental biological processes.…

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A: Cell viability assays are the assays which are mainly used to determine or find out the number of…

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Q: Describe the banding pattern seen in the gel. What does that tell you about the role of annealing…

A: Gel electrophoresis is a laboratory technique for separating charged molecules such as DNA, RNA, and…

Q: A researcher is interested in using the in vitro technique of bisulfite conversion to confirm the…

A: DNA methylation is a chemical change that frequently takes place in the setting of CpG dinucleotides…

Q: In DNA purification, which step facilitates the separation of DNA from RNA? Also, what is the…

A: We have to learn how to clean DNA and split it from RNA. The question is asking which part of the…

Q: Biochemistry: Site-directed mutagenesis, in which individual amino acid residues are replaced with…

A: Enzyme activity is used to assess the catalytic activity of an enzyme. One enzyme unit (U) is the…

Q: In describing the triplet binding assay, an undergraduate with limited knowledge of translation…

A: Every three nucleotides in a row count as a triplet in the genetic code and codes for a single amino…

Q: Polymerase Chain Reaction (PCR) was invented by Kary Mullis in 1983. This technique had indeed…

A: The annealing temperature is the temperature at which the primers bind to the denatured DNA strands.…

Q: how an ion-selective field effect transistor (ISFET) can be used as a biosensor to detect DNA in…

A: An ISFET biosensor can be used to detect DNA in solution by detecting changes in the pH of the…

Q: What is the advantage of homology-directed repair (HDR) ?

A: One of the most vital cellular processes called homology-directed repair (HDR) is capable for fixing…

Q: What are the advantages of using a restriction enzyme whose recognitionsite is relatively rare? When…

A: Restriction enzymes or restriction endonucleases are enzymes capable of recognizing a specific…

Q: Prior to the publication of the article from which this assay comes from (picture), the Cas9…

A: SDS-PAGE Analysis of Cas9 Orthologsa. Experimental Question and HypothesisResearch Question: The…

Q: What are DNA probes

A: When two single-stranded DNA or single-stranded RNA molecules are allowed to be together and form…

Q: What functional information about a genome can be determined through applications of chromatin…

A: Chromatin Immunoprecipitation (ChIP)  is a type of technique based on immunoprecipitation and is…

Q: What is the function of CTAB being added to the cell culture before performing the enzyme induction…

A: Cell culture is the propagation of cells by providing a controlled physiological and physicochemical…

Q: A recombinant protein corresponding to 52 repeats of the peptide sequence YSPTSPS was covalently…

A: The peptide sequence YSPTSPS is a known phosphorylation site that corresponds to the heptad repeat…

Q: Experiment: Transformation of the bacterium Streptococcus pneumoniae. Based on the study, four…

A: Introduction The genetic modification of a cell caused by the direct uptake and integration of…

Q: In an experiment assay, sometimes there are several dilutions involved. 0.5 mL of (Bovine serum…

A: Dilution is responsible for decreasing the solute concentration in the solution.

Q: Why is the DNS method used in glucose assay?

A: 3,5-dinitrosalicylic acid (DNS) measure has been broadly utilized for the estimation of diminishing…

Q: position of the Start and Stop. The GFP and RFP genes shown do not have start

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Q: What is meant by the term genetic testing? How do testing at theprotein level and testing at the DNA…

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Q: In your own words, explain the term transposon tagging.

A: Transposons or jumping genes are the sequences of DNA that are able to change their position within…

Q: Can you please explain what is happening in this figure? Please address controls, experimental…

A: An article titled "Distinct sets of enhancers activate transcription from the hkCP and dCP in S2…

Q: What does MEGA stand for and what is this assay used to determine? How to do perform the assay?

A: Many assisted reproductive techniques are widely used around the globe.

Q: You have two E. coli cultures. One culture is a wild-type culture, and the other contains the lacOc…

A: Escherichia coli (E. coli) is a type of bacteria that is commonly found in the lower intestine of…

Q: The carboxy terminus of the p53 protein acts as an allosteric regulator of sequence-specific DNA…

A: General structure of p53 protein

Q: E. coli K12 genomic DNA (expected PCR product was 3075bp). For expression the gene was cloned into…

A: The information you provided suggests that a gene was cloned into the expression plasmid pET28a, and…

Q: Questions are talking about amplification of DNA When we run the gel, we also run a ladder why?

A: A DNA ladder is the mixture of amplified DNA fragments of different size range which is used as…

Q: Can you explain what is nucleotide selectivity in polymerase

A: One of the structural elements, or building blocks, of DNA and RNA is the nucleotide. A nucleotide…

Q: hat is an ONPG test? WHat is he principle behind this assay.

A: Fermentation of lactose happens by bacteria which depends on two enzymes - permease (allows lactose…

Q: Explain the Triplet Binding Assay ?

A: Introduction  In 1964, two eminent scientists Marshall W. Nirenberg and Philip Leder carried out the…

Q: Some compounds are "frequent hitters" or pan-assay interferences (PAINS). Does having a really…

A: PAINs (Pan-Assay Interference Compounds) are small molecule compounds that have been found to…

Q: Why is the bradford assay method using BSA sensitive ? and how does it compare to the amino acid…

A: Bradford assay: It is a widely used method for measuring total protein concentration and different…

Q: Describe the rationale behind the electrophoretic mobility shift assay.

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Q: Give three reasons why liposomes/nanoparticles are attractive drug delivery system for recombinant…

A: Introduction: Liposomes are a small-sized vesicle sharing structural and compositional similarity…

Q: The excision of benign or malignant lesions cannot be reported in addition to which of the following…

A: Repair and closure (12001 - 13160)

Q: Prior to the publication of the article from which this assay comes from (picture), the Cas9…

A: In the graphical representation of SDS-PAGE analysis, separate bands corresponding to various Cas9…

Q: What is the role of streptomycin in CRISPR experiment? What biochemical changes (DNA, protein)…

A: The genetic tool CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) has…

Q: Preparing plasmid (double-stranded, circular) DNA for sequencing involves annealing a complementary,…

A: The process of joining of single-stranded DNA or RNA by hydrogen bonds to form a double-stranded…