What assay uses the fact that activators can be separated into DNA binding and activating domains? What information do you gain from this assay? Describe briefly how to perform this assay.

Q: During Ames assay the colonies that grow on His- plates (no amino acid His was present) from the…

A: Ames test is used to detect potential mutagenic compounds or chemicals. These chemicals can also be…

Q: Describe the banding pattern seen in the gel. What does that tell you about the role of annealing…

A: Gel electrophoresis is a laboratory technique for separating charged molecules such as DNA, RNA, and…

Q: A researcher is interested in using the in vitro technique of bisulfite conversion to confirm the…

A: DNA methylation is a chemical change that frequently takes place in the setting of CpG dinucleotides…

Q: What is the point of hydrolyzing RNA before doing biochemical tests?

A: Asked: The reason of hydrolyzing the RNA before doing biochemical tests.

Q: Biochemistry: Site-directed mutagenesis, in which individual amino acid residues are replaced with…

A: Enzyme activity is used to assess the catalytic activity of an enzyme. One enzyme unit (U) is the…

Q: What is a restriction enzyme? What structure does it recognize?What type of chemical bond does it…

A: Werner Arber, a Swiss microbiologist, winner of 1978 Nobel Prize in Physiology, for his discovery…

Q: What is the possible cause of the presence of an extra band in the results of DNA extraction and PCR…

A: This finding suggests that formation of multiple bands in non-denaturing gel electrophoresis is a…

Q: ndom mutations anywhere within the genome in living cells (in vivo)           b) random…

A: In research to create mutations it is very necessary to first decipher the type of organism in which…

Q: How are probes used to screen DNA libraries? Explain how a synthetic probe can be prepared when the…

A: A probe is an oligonucleotide stretch of DNA (Deoxyribonucleic Acid) or RNA (Ribonucleic Acid) which…

Q: What is the attached enzyme in this assay and its corresponding substrate? A. Horseradish peroxidase…

A: INTRODUCTION ELISA Enzyme linked Immunosorbent assay, is a technique used to detect antibodies in…

Q: What is the advantage of homology-directed repair (HDR) ?

A: One of the most vital cellular processes called homology-directed repair (HDR) is capable for fixing…

Q: What are the advantages of using a restriction enzyme whose recognitionsite is relatively rare? When…

A: Restriction enzymes or restriction endonucleases are enzymes capable of recognizing a specific…

Q: What are DNA probes

A: When two single-stranded DNA or single-stranded RNA molecules are allowed to be together and form…

Q: What is transformation efficiency and how is it calculated?

A: Transformants are the cells that are able to incorporate any modified foreign or artificial DNA.…

Q: ld telomerase inhibitors be used to treat cancer?

A: Telomerase, also known as  terminal transferase can be described  as the  ribonucleoprotein that…

Q: Why is the DNS method used in glucose assay?

A: 3,5-dinitrosalicylic acid (DNS) measure has been broadly utilized for the estimation of diminishing…

Q: What is meant by the term genetic testing? How do testing at theprotein level and testing at the DNA…

A: Medical genetics is one of the branches of medicine, which involves the management and diagnosis of…

Q: What does MEGA stand for and what is this assay used to determine? How to do perform the assay?

A: Many assisted reproductive techniques are widely used around the globe.

Q: What is a microsatellite?  Draw what a microsatellite looks like in a zone of inhibition in a disk…

A: Microsatellite- It is repetitive DNA in which motifs of DNA are repeated.

Q: What is the aim of hydrolyzing the RNA before doing biochemical tests?

A: RNA hydrolysis: It is a process of breakdown/digestion of RNA molecules by breaking phosphodiester…

Q: can you explain why we are hoping to obtain white colonies? (Please explain both why blue colonies…

A: In recombinant DNA technology vector is any DNA molecule either from plant, virus, plasmid or…

Q: Briefly explain how synthetic probes are created to screen a DNA library when the protein encoded by…

A: Synthetic probe is fragment of DNA. to screen a DNA library RNA of variable length approximately 100…

Q: what are similarities and differences between chain-termination and reversible terminator…

A: Gene sequencing  aids in the development of gene therapy, a form of treatment that replaces faulty…

Q: the differences between THT and SMT PCB fabrication

A:

Q: How can we use the transposon sequence to find where the mutation occurred in a forward genetic…

A: Forward genetic screes, also termed as forwarding genetics, is a kind of process by which those…

Q: How is telomerases related to ageing? How Can telomerase- related ageing be addressed…

A: Humans lose physiological and psychological function as they age, which is a normal process. The…

Q: E. coli K12 genomic DNA (expected PCR product was 3075bp). For expression the gene was cloned into…

A: The information you provided suggests that a gene was cloned into the expression plasmid pET28a, and…

Q: Can you explain what is nucleotide selectivity in polymerase

A: One of the structural elements, or building blocks, of DNA and RNA is the nucleotide. A nucleotide…

Q: hat is an ONPG test? WHat is he principle behind this assay.

A: Fermentation of lactose happens by bacteria which depends on two enzymes - permease (allows lactose…

Q: How would you determine the concentration of a protein that falls outside (above or below) the…

A: Introduction A biomolecule, sometimes known as a biological molecule, is any of the various…

Q: Explain the Triplet Binding Assay ?

A: Introduction  In 1964, two eminent scientists Marshall W. Nirenberg and Philip Leder carried out the…

Q: Some compounds are "frequent hitters" or pan-assay interferences (PAINS). Does having a really…

A: PAINs (Pan-Assay Interference Compounds) are small molecule compounds that have been found to…