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What is the function of a mordant, and which reagent serves this purpose in the
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- Explain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a dayDescribe the procedure for performing a Gram stain. What is the procedure? How does each component work? (ie. What does the mordant do?)What is the purpose of the alcohol (OH) in the gram staining process? Explain.
- About the Gram staining technique, explain: What is the purpose of using lugol and alcohol in this type of coloring?Name all the reagents used in Gram-staining and their roles in the staining procedure.Answer the following questions What is the purpose of the 95% ethanol step in the Gram staining procedure? (1 Mark) xiii) What is the function of the following reagents used in Gram stain: a) Crystal Violet b) Gram’s iodine c) Acetone/alcohol d) Safranin I
- What is the function of the mordant used in the Gram staining procedure? If the iodine step is skipped, what color are Gram-negative cells observedWhat is the difference between simple and differential stains? Give an example of a differential stain.In Gram staining method, list the reagents and state their role for each step of the procedure? In preparing bacterial smear, why do we need to pass the slide over the flame?