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- What can be the errors and limitations of Gel Electrophoresis practical? Explain in very much detail and also provide some better suggestions.Briefly discuss the difference between simple and differential staining? Which is better suited for common clinical microbiology laboratory applications?Select all that apply to a direct simple stain: 1. the dye is acidic or negatively charged and stains the background 2. uses multiple dyes in the procedure 3. cells will never be distorted or shrunken 4. uses only 1 dye in the procedure 5. does involve a washing step 6. can be used to determine cell morphology, size, and arrangement. 7. does involve fixing 8. the dye is basic or positively charged and stains the bacteria 9. cells may be distorted or shrunken 10. does not involve a washing step 11. does not involve fixing
- name 5 things about method staining.The functions of the loading dye are: (select all that apply) to prevent sample degradation as gel generates heat to make sure sample sinks to bottom of sample wells so that you can visualize the sample as it runs through the gel so that you can see your bands under UV lightWhat is the main advantage of fluorescence polarization? Sensitivity of fluorescent probes Low cost of instrument and reagents Compatibility of reagents with most analytes No need to purify samples Easy to attach fluorescent probe
- *Pure culture* o What is the desired outcome (result) of a streak plate? o Clinically, why is it important to make a pure culture from a clinical specimen? What do you do next in order to treat the patient?How does smear preparation of cells from a liquid medium differ from preparation of cells from a solid medium? Mark all that apply: A smear made from liquid media will be thinner than a smear made from solid media A smear made from solid media doesn't have to be fixed but a smear from liquid media does A smear made from liquid media will be more thick than a smear made from solid Water must be added to a smear made from solid media but not liquid media A smear made from liquid media doesn't have to be fixed but a smear from solid media does Water must be added to a smear from liquid media but not from solidDescribe how simple staining and fluorescence staining are similar and how they are different. What are the advantages of each?
- What is gel electrophoresis and why is it important? Write in detail.c: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain. Give one example. e: Name two blood parasites that can be demonstrated using the Giemsa stain.How is fluorescence spectroscopy beneficial for pharmacological analysis ? Please shortly answer at your own words. Answer should be to the point(specific 4-5 lines).