u have assembled a new prokaryotic genome and are annotating the genes.  If you were to search for highly conserved sequences necessary for normal gene expression, what type of annotation are you doing? Group of answer choices ad hoc annotation homology based annotation preliminary annotation ab initio annotation

Genetic engineering and gene therapy are two issues in genomics that are quite similar. What similarities and differences do they share?

Now you have the gene sequence. Now you would like to clone it into an expression vector to grow up in a bacterial system. Because you're going to use bacteria to generate protein from a eukaryote, the mammoth, you need to get rid of introns from your sequence. How do you do that?     Bioinformatically, I look for splice-site sequences and branch-point adenines and predict intron-exon boundaries     I use a comparative genomic approach and use sequence homology with the genome of a closely related species     I use a comparative genomic approach and use sequence homology with the genome of a distantly related species     Both A and B     Both B and C   Why did you bother to identify the introns?     So that I could include them in the sequence to understand intron function.     So that I could exclude them from the sequence because prokaryotes don't have spliceosomal machinery.     So that I could see how introns affect protein folding.

For a common bacterial gene with a promoter, match each mutation to its most likely effect. Increased rate of transcription -11 up mutation -36 down mutation Decreased rate of transcription +1 mutation. Answer Bank -20 mutation No effect on transcription Question Source: Pierce 6e-Genetics A Conceptual Approach Publisher