Which of the following is TRUE of next generation sequencing technology? It is required for CHIP-seq and RNA-seq It can be used to directly sequence RNA It has facilitated an explosion of publicly available genome sequences for eukaryotes and prokaryotes It is extremely expensive as compared to previous sequencing approaches Illumina is a next generation sequencing platform It can be used to sequence proteins (proteomics)

Q: NOT a DNA-based method

A: This is a method which refers to the technique or approach that utilizes the DNA molecules or…

Q: During pcr primers can:  become graded by rnases be covslently linked to the template dtrand fall…

A: PCR stands for polymerase chain reaction. It is used to amplify the specific region of gene.

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A: In E. coli, lactose is the substrate for the enzyme beta-galactosidase and it regulates the…

Q: Recombinant DNA Technology Provide a detailed explanation how DNA structure is exploited for the…

A: DNA Sequencing:                   A laboratory technique used to determine the exact sequence of…

Q: Which of the following would be the best assay to use to determine if a protein binds to RNA? O…

A: Gel electrophoresis is a laboratory technique used to separate and analyze charged molecules, such…

Q: In this lab you learned about purifying DNA from a soil sample using a series of columns and…

A: INTRODUCTION DNA purification DNA purification helps in the extraction of genomic or plasmid DNA. It…

Q: Mapping high-throughput sequencing reads (choose all that apply) O requires a special device called…

A: Mapping high throughput sequencing refers to the amount of DNA molecules that read at the same time.…

Q: I am having issues with differentiating between the different types of PCRs and why the different…

A: The polymerase chain reaction (PCR) is a technique in which several million copies of the desired…

Q: What is the last step in metagenomics? Breaking open the bacterial cells Assembling short DNA…

A: Hello. Since your question has multiple parts, we will solve first question for you. If you want…

Q: The Human Genome Project attempts to modify organism's DNA inserting desired genes into bacteria.…

A: The gene is known as the unit of heredity, and this unit is both physical and functional as these…

Q: DNA extraction from banana is being analyzed

A: The stringy substance that you see is DNA! It has been removed from the millions and millions of…

Q: ase enzymes are used in recombinant dna technology

A: Restriction enzymes (also called restriction endonucleases) cleave double-stranded DNA at very…

Q: Which of these is not a tool for comparing DNA sequences?   BLAST Fasta PLINK A dotplot e.g.…

A: The amount of data generated by next-generation sequencing platforms is enormous. The information…

Q: DNA fingerprinting is most associated with which of the following? Northern blotting Western…

A: DNA fingerprinting or DNA profiling is a technique that is used to generate specific characteristics…

Q: DNA template A complementary DNA primer Single-stranded RNA template Four deoxynucleotides (DATP,…

A: Introduction:- The process of determining the sequence of nucleotides (As, Ts, Cs, and Gs) in a…

Q: CRISPR-CAS9 is a: s a: alien weapon to control our minds a eukaryotic gene protein matrix type of…

A: CRISPR-Cas9, which is short for clustered regularly interspaced short palindromic repeats and…

Q: In a PCR reaction to amply a particular gene, 120 copy of the gene were initially used as DNA…

A: Here, option A is the right answer. You may use the following formula to calculate the number of…

Q: Primer annealing is an important aspect of PCR. The annealing step of the cycle usually takes place…

A: Annealing refers to the process in which single-stranded DNA or RNA molecules bind or hybridize with…

Q: Which of the following is the sequencing gold standard? Oion semiconductor sequencing O sequencing…

A: DNA sequencing is the process of determining the precise order of nucleotides (A, T, C, and G) in a…

Q: ere are several different tools used to study or manipulate DNA. Match the description to the…

A: The field of biotechnology has evolved enormously over the years. it's gone from the normal read of…

Q: What's the main difference between first-generation sequencing, second-generation sequencing and…

A: DNA sequencing is a research center procedure used to decide the specific arrangement of bases (A,…

Q: Describe the 2 main types of sequencing prior to next generation sequencing.

A: DNA sequencing:As the name suggests "DNA sequencing" is a method that can help us determine the…

Q: Draw an annotated diagram outlining the steps involved in a Next Generation sequencing technology of…

A: In next-generation sequencing, the advantage is that the data result are shorts, readable and many…

Q: Sanger Illumina PacBio Amount of DNA needed for sequencing Read length Amount of data sequenced…

A: DNA sequencing DNA sequencing involves various techniques by which the order of nucleic acid…

Q: What are some of the observations and conclusions that can be made from gel bands for 16s PCR

A: Answer :- Here are some observations :- 1) Increase hardening temperature by increments of two…

Q: What is the program Finch TV used for? Viewing next generation sequencing data Opening sequencing…

A: Ans- C EXPLANATION- FINCH Tv is the tool or application used by the researchers to view, analyze,…

Q: Why is it important to sequence positive clones derived from PCR cloning? Group of answer choices

A: PCR is a technique of amplification of a given molecule of DNA to increase its copy number.

Q: Which of the following statements is true about consensus sequences in DNA? Group of answer choices…

A: Short nucleotide segments which recur often in conserved sequences are known as consensus sequences.…

Q: The algorithm or tool in MEGA that is used for multiple sequence alignment of nucleotide/DNA…

A: Introduction In bioinformatics MSA (Multiple Sequence Alignment)  is generally the alignment method…

Q: Running a PCR Why is a Hotstart necessary and how is it achieved? Why are the three temperatures…

A: Answer: 1)In order to avoid non-specific DNA amplification, hotstart is required in PCR. The…

Q: 1 Gene annotation is synonymous to gene sequencing. (True or false 2 A chromosome is smaller than a…

A: Gene Annotation : The process of identifying different genes and determining their function and…

Q: Which of the following is not a component of Sanger sequencing? OddNTPs with fluorescent tags…

A: Question 1 explantion: The component not typically used in Sanger sequencing is restriction enzymes.…

Q: After sequencing a new genome for the first time, functional protein-coding genes are initially…

A: Gene is defined as the basic unit of functional and physical part of heredity. Genes helps to make…

Q: Which of the following are true about ddNTPs. Select all that apply. They cause DNA synthesis to…

A: Dideoxynucleotides are nucleotides that lack a 3'-OH group, which helps in the termination of DNA…

Q: Objective: Get a sense of how genomics, the study of the genome in its entirety, needs to think…

A: The analysis of the total genome in genomics research requires careful evaluation of sequencing…

Q: Order the following technical steps that are required to identify a bacterium by PCR and sequencing…

A: The objective of the question is to arrange the given steps in the correct order that are required…

Q: What are the different types of PCR and their application?  Please answer at your own words

A: PCR : Polymerase chain reaction - It is an efficient and cost effective molecular tool to copy or…

Q: Which of the following is a unique attribute of CRISPR/Cas9 compared to restriction enzymes Group of…

A: The objective of the question is to identify the unique attribute of CRISPR/Cas9 compared to…

Q: All things considered, the most important factor with respect to successful PCR is. Multiple Choice…

A: PCR, or Polymerase Chain Reaction, is a molecular biology technique for making multiple copies of a…

Q: What molecule is next generation sequencing analyzing? DNA, RNA, or protein

A: What is next generation sequencing? It is the process of massive parallel sequencing of DNA…

Q: Any molecular project, whether it be genetic engineering, phylogenetics, population genetics,…

A: We can strongly agree with this point.  Any molecular project, whether it be genetic engineering,…

Q: In which sequencing method, DNA is labelled and then chemically cleaved in a sequence-dependent…

A: DNA sequencing refers to decoding the base sequence of the DNA strand. It tells us the base…

Q: Which of the following is FALSE of functional genomics? Select ALL that apply. RNA seq is a…

A: Functional genomics - It can be defined as the study which is based on how intergenic regions as…

Q: 1. What is the difference between an iterated blast (psi-blast) search and a simple blast search? 2.…

A: Hello. Since your question has multiple parts, we will solve the first question for you. If you want…

Q: You culture bacteria from the soil at a toxic waste dump on an agar plate and pick a single…

A: PCR, including Real-Time PCR which is probably the most widely used molecular technique especially…

Q: Which of the following describes forward genetics? Utilizing consensus regions within a genome to…

A: Genetics is the branch of biology that deals with the study of genes, their inheritance patterns,…

Q: Genomics and proteomics give us insights into genome function.  True  False

A:

Q: Why amplify DNA using PCR before doing electrophoresis? Why not just take your DNA sample and…

A: The polymerase chain reaction (PCR) is a typical laboratory technique for making multiple copies of…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.