A patient is diagnosed with lung cancer using imaging. To establish the best treatment option, doctors ask to profile the gene expression of the tumor, using a biopsy and microarray analysis. Describe the steps involved in the analysis in detail. The analysis reveals that a new gene therapy could be beneficial to the patient. Explain briefly how gene therapy could be used to cure cancer and detail how the treatment can be formulated for delivery to the patient. To prepare DNA for the gene therapy formulation, it is often important to measure the size of the molecule. Explain how this measurement can be done using agarose gel electrophoresis, taking care to detail the property of the DNA molecule at the basis of the technique.
A patient is diagnosed with lung cancer using imaging. To establish the best treatment option, doctors ask to profile the gene expression of the tumor, using a biopsy and microarray analysis. Describe the steps involved in the analysis in detail. The analysis reveals that a new gene therapy could be beneficial to the patient. Explain briefly how gene therapy could be used to cure cancer and detail how the treatment can be formulated for delivery to the patient. To prepare DNA for the gene therapy formulation, it is often important to measure the size of the molecule. Explain how this measurement can be done using agarose gel electrophoresis, taking care to detail the property of the DNA molecule at the basis of the technique.
Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter16: Reproductive Technology, Genetic Testing, And Gene Therapy
Section: Chapter Questions
Problem 12QP: In selecting target cells to receive a transferred gene in gene therapy, what factors do you think...
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Transcribed Image Text:A patient is diagnosed with lung cancer using imaging. To establish the best treatment
option, doctors ask to profile the gene expression of the tumor, using a biopsy and
microarray analysis. Describe the steps involved in the analysis in detail.
The analysis reveals that a new gene therapy could be beneficial to the patient. Explain
briefly how gene therapy could be used to cure cancer and detail how the treatment
can be formulated for delivery to the patient.
To prepare DNA for the gene therapy formulation, it is often important to measure the
size of the molecule. Explain how this measurement can be done using agarose gel
electrophoresis, taking care to detail the property of the DNA molecule at the basis of
the technique.
To generate large amounts of DNA to manufacture gene therapy payloads or to be
able to see them on gel electrophoresis, specific sequences can be amplified by PCR.
For a sequence of a 100 base pairs, calculate the number cycles of PCR required to
generate 1 ng of DNA, when starting from a single copy.
Note that the molar mass of an average DNA bp is 600 g/mol.
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