transfer and isolation techniques: When getting inoculum from a slant, why is it necessary to touch a sterile part of the agar with the loop before touching the bacterial growth?
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transfer and isolation techniques:
When getting inoculum from a slant, why is it necessary to touch a sterile part of the agar with the loop before touching the bacterial growth?
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- procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (3) agar plate to agar slant.procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (2) agar slant culture to agar slantLoop with bacterial inoculum was subjected to flame sterilization prior to streaking on agar plate. Effect: Rationale:
- Why should bacterial culture plates be incubated inverted (bottom up)?Technique used to inoculate plate media using inoculating loop. Technique used to inoculate agar deep using inoculating needle. Pattern used to inoculate agar slant to get luxuriant culture. This pattern increases the surface area of agar that can be inoculated. Can you answer all the questions? Thankyou!Bacterial cells can form colonies on the following media EXCEPT: Select one: Nutrient broth Agar slant Agar plates Agar deep
- What is the major difference between an enrichment culture and a selective culture? Why are microbial doubling times in nature typically longer than those obtained in the lab? Briefly describe the following mechanisms of measuring bacterial growth: Direct microscopic cell count Plate count Most probable numberIn a bacterial culture on an agar plate, what is a zone of inhibition? An area where the growth of bacteria is increased compared to the rest of the agar plate An area where the growth of bacteria is prevented or reduced compared to the rest of the agar plate An area where the growth of bacteria is not affected compared to the rest of the agar plate An area where the the agar becomes contaminated with moldLid of petri dish was left on bench top for a few minutes after pouring of sterile medium. Effect: Rationale:
- microbial sensivity lab: in the procedure used to test bacterial growth against various temperatures (incubator, room, refrigerator, freezer), why should efforts be made to inoculate each tube with the same number of bacteria?What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?Subculture and Colony Morphology Descriptions Following identification of the Gram-negative isolate and Gram-positive isolate, you next subculture each onto fresh nutrient agar plates. Briefly describe the subculture process in three or so sentences and what this allowed you to achieve; follow this with colony morphology descriptions of each. Description: Isolate A – Describe: Colony morphology: Medium & incubation temperature: Isolate B – Describe: Colony morphology: Medium & incubation temperature: