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- Question 14 Which of the following tests requires a dilution of the culture before inoculation? O Bacitracin Bile esculin agar O DNase O CoagulaseSEPARATION OF COMPONENTS BY CENTRIFUGATION Consider the following subcellular components and their corresponding sedimentation rates. Table 3. Sedimentation rates of four subcellular components for isolation. CENTRIFUGAL FORCE (xg) SUBCELLULAR COMPONENT CDCP 14896 AJRA 967 AGC 382 JCKSP 2378 Two centrifuge machines are available in your laboratory. Centrifuge A has a rotor diameter of 14 cm and a maximum speed of 3500 rpm while centrifuge B has a rotor diameter of 18 cm and a maximum speed of 14500 rpm. Construct a separation scheme of the four subcellular components considering the following parameters: The four components are separated into pellet/supernatant I and pellet/supernatant II. Only two centrifugation steps are performed; specify the centrifugal speeds (in rpm) per step. Show relevant calculations. Both centrifuge machines must be used in the procedure. Components of each fraction are indicated.In transformation, donor__________ in the growth mediumenters a recipient cell.
- I need help with the following question: After graduating from university you decide to celebrate your success with a trip to Thailand during the summer. You rent a moped to visit an archeological site on the outskirts of Bangkok. On the way there you hit a patch of gravel and wipe out, badly scraping your leg. Lurking in the dirt embedded in the scrape on your leg is Burkholderia pseudomallei, a soil bacterium endemic in Southeast Asia but not present in North America. Describe the cellular and molecular processes necessary for the activation of a primary adaptive immune response and the effector mechanisms produced to clear the infection. Be specific in terms of receptors and effector molecules specific to the type of pathogen.Question:- Define the bacterial categories that each type of bacteria belongs to. You only have to name the categoriesNOT the actual species of bacteria Note: there will be more than one category . A bacterial species that grows best at 37 degrees Cand when a small amount of oxygen is presentcontinuous disc stack centrifuge is operated at 5000 rpm for separation of tukers' yeast. At a feed rate of 60 min21, thirty of the cells are recovered. For operation at constant centrifuge speed, solids recovery is inversely proportional to the flow rate. (a) What flow rate is required to achieve 90% cell recovery if the centrifuge speed is maintained at 5000rpm thi What operating speed is required to achieve 90% recovery at a feed rate of 60 min211).
- Numerous mechanisms are used by the different eukaryotic microbes for cellular locomotion. Please discuss at least 4 of these mechanisms for locomotion utilized by these cells for movement through their microenvironments. Be sure to state the specific organism (name drop please) that you are referring to and how this locomotion mechanism is used by the cell and by microbiologist for classifying microbial cells.E. coli has a doubling time of 0.345 hours at 37 0C. Starting with 1 milligram of cells, assuming a lag phase of 30 minutes and a stationary phase of 30 minutes, what number of cells would you expect at the end of 13h, assuming that besides the lag and stationary phases the culture is continuously in exponential phase. Assume that ln (2) = 0.69 and that the mass of a single cell is 10^(-12) g.Bacteria grow in a nutrient solution at a rate proportional to the amount present. Initially, there are 250strands of the bacteria in the solution which grows to 800 strands after seven hours. Show (a) anexpression for the approximate number of strands in the culture at any time t and (b) the time needed forthe bacteria to grow to 1600 strands.
- The figure above depicts an agar cube with a side length of 13\, \text{mm}13mm13, start text, m, m, end text. In an experiment, students submerged the cube in red dye for 121212 hours. The red dye permeated 1\, \text{mm}1mm1, start text, m, m, end text on each side, as indicated by the shading in the figure. Volume of a rectangular solid: V = lwhV=lwhV, equals, l, w, h Calculate the volume of the agar cube that remained unpenetrated by the red dye.Antibiotic Code Zone of Inhibition (mm) S, I, or R? Penicillin P 15 Streptomycin S 8 Ampicillin A 25 Chloramphenicol C 14 Bacitracin B 5 QUESTIONS: Which antimicrobial agent was most effective against your organism? Can you determine from your results whether this antimicrobial agent will also be most effective against another bacterium? Why or why not? Is this technique measuring bacteriostatic effect, bactericidal effects, or both? Explain. 4) Consider the following list of hypothetical antibiotics, tested against Escherichia coli in a Kirby-Bauer test: Antibiotic Zone of Inhibition (mm) Astonostatin 12 Bodaciosporin 25 Crazifloxacin 5 Dorkimycin 16 When the above antibiotics were tested as treatments for E. coli infections in humans, it was found that Dorkimycin has virtually no effect on the infection, while Astonostatin is the most effective of the four antibiotics listed. Propose an explanation for this result. Based purely on these…procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (2) agar slant culture to agar slant