procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (3) agar plate to agar slant.

Q: Calculate the CFU/ml of leuconostoc in the brine for the 4 different days. Show your calculation

A: CFU/ml stands for "colony forming units per milliliter." It is a unit of measurement used in…

Q: Using one of the graphs obtained with the medium II, name the different phases of the bacterial…

A: Micro organisms are typically grown in a closed culture media. Nutrients are added according to the…

Q: Given: The used ocular objective while taking this image, has magnifying power of 6x The used…

A: Given : Stage micrometer used has graduation = 0.01mm Ten graduation on ocular micrometer coincides…

Q: Explain the expressions of primary culture, cell haH, and continuous cell haH. Explain the reason…

A: Answers :  Th cells in the primary culture are heterogeneous as they are freshly obtained from the…

Q: In a fed-batch culture operating with intermittent addition of lactose solution, values ofthe…

A: Note: As per Bartleby Guidelines For Remaining Answers Please Repost The Question. Introduction:  A…

Q: Need help  Can a pure culture be prepared from a mixed culture in a broth or plate? Explain.

A: INTRODUCTION How to make a pure culture from mixed culture is explained below.

Q: Why is it sufficient to stain cells from a SDA (Sabouraud Dextrose Agar) plate with crystal violet…

A: Introduction : Sabouraud agar, often known as Sabouraud dextrose agar (SDA), is a peptone-containing…

Q: ou've been tasked with observing the motility of an UNKNOWN Bacteria isolated from a mixed culture.…

A: Bacteria is the tiny cellular living structure which belong to the  Moneran kingdom. The bacteria…

Q: identify the organisms in  an unknown culture. You find that the culture contains two gramnegative…

A: A culture is a medium to grow bacteria and other microorganisms  It provides the bacteria with…

Q: Drawings of the two bacterial cultures as observed under the 4 mm objective. Describe features…

A: Staphylococcus aureus and Streptococcus pyogenes are gram positive bacteria that cause infections in…

Q: For a laboratory exercise of basic techniques in microbiology: Gram stain and Microscopy. What are…

A: "Gram staining is important in microbiology because it allows scientists to quickly and easily…

Q: If each agar plate contained 20 ml of LB agar and had an ampicillin concentration of 150 ug/ml, what…

A: Given data:  Each plate contains-  LB agar=20ml Ampicillin= 150 micrograms per ml.  Amount of…

Q: Both the Endospore Stain Protocol and Mannitol Salt Agar Media are differential. How can this be so?

A: The growth media are nutritious media that are used to grow microbes (bacteria and fungus) in the…

Q: Sulfur Indole Motility (SIM) Medium H2S produced, color and +/-: ______________________________…

A: Sulphur indole motility medium used to test the ability of am organism to have properties of…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: You are givena mix culture of S. aureus, E. coli and P. aeruginosa. Besides using the streak plate…

A: The microorganisms can be differentiated from each other based on their growth characteristics,…

Q: Biochemical and differential tests can be incorporated into growth media or performed on isolated…

A: It is required to provide three examples of differential test that is incorporated into growth media…

Q: How could calculate this serial dilutions probelms for microbiology and how do can I know what tube…

A: Serial dilution is a method of step-wise dilution of bacterial culture to reduce the concentration…

Q: What are the difference in the movement of microorganisms under wet mount from hanging drop…

A: There are some plus point for both the techniques like the wet Mount and hanging drop method…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: Based on the picture write description changes in the morphology of the cultures and the presence of…

A: Plant tissue culture (PTC) refers to in vitro culturing of plant seeds, explants, plant organs,…

Q: The students of a Microbiology class were tasked to transfer or subculture a pure culture of…

A:

Q: How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the…

A: Serial dilution is used mostly in studies involving cell cultures (microbiology or even cell culture…

Q: What are the principle and basic concepts of SMEAR PREPARATION? (please explain it thoroughly in a…

A: Introduction A smear is a small amount of culture spread in a very thin film on the surface of the…

Q: Identify the texture, transparency, color, colony size, form, margin and elevation of the two…

A: A "colony" is a collection of bacteria, fungus, and other microorganisms cultured on solid agar…

Q: Answer the following questions briefly and concisely 1.How do bacteria in a chemostat and those in…

A: If the dilution rate in a chemostat is higher than the organisms maximum specific growth rate it…

Q: procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (1)…

A: Aseptic technique is most commonly used in microbiology and biotechnology lab. It refers to the…

Q: Using saline tubes containing 9 ml of saline (for dilutions), draw a diagram that would yield…

A: All the bacteria in a colony are identical as they arise from a single bacterium. Thus, a colony is…

Q: This test determines the ability of bacteria to use citrate as source of carbon and inorganic…

A: The citrate test is utilized to check whether a bacterium species can use citrate as a source of…

Q: Voges Proskauer test Does color of the medium change to red after both reagents were added and…

A: Biochemical method of bacterial identification is a classical method that is used for the…

Q: For the following agar media used, list their significant components and explain how they react.…

A: The sheep blood agar is a popular agar medium. It is a differential medium that includes important…

Q: Medium used for cellulase production contained (g/L): NaNO3, 3; MgSO4 7H2O, 0.5; K2HPO4, 1; KCl, 0.5…

A: Culture media is proper nutrient for the growth of the microorganisms, which help to grow a variety…

Q: In the preparation of a pure culture media, the petri plates are inverted when placed in an…

A: In microbiology, culture media is considered the growth medium that possesses the specific mixtures…

Q: Given the scenario, compute for the total volume of the culture media solution (milliliter or liter)…

A: We have been given the concentration of the nutrient broth and Agar and we have also been given the…

Q: A sample of Earth Alive Soil Activator was diluted by transferring 1 gram into a 9 ml dilution blank…

A: Microorganism concentrations are calculated using serial dilutions. A viable bacterial or fungal…

Q: Why should bacterial culture plates be incubated inverted (bottom up)?

A: Microbial isolation is a process done in microbiology lab in order to obtained microbe of interest…

Q: A PhD student leaving for vacation has asked an undergraduate student to perform daily media changes…

A: A mathematical model known as Michaelis-Menten kinetics is used to explain enzyme kinetics. It is…

Q: In aseptic technique, what will happen if the inoculated solid plated media is not inverted prior to…

A: Culture Media   Culture media also known as growth media may be liquid,  semisolid, or solid, is use…

Q: microbial sensivity lab: in the procedure used to test bacterial growth against various temperatures…

A: Growth of bacteria differs with varying temperature. Bacteria grows rapidly in the temperature range…

Q: What is the main difference between plates before and after centrifugation? What may be the purpose…

A: Answer: CENTRIFUGATION = It is the process involving mechanical force that is centrifugal force,…

Q: You just finished plating your electroporatio products on your YPD-Zeocin plate and you think you…

A: Electroporation is a technique used to introduce foreign DNA into cells by applying an electric…

Q: The student advises using the same selection procedures and inoculating a 5 mL overnight culture…

A: Quantification: The process of plating on agar plates enables the counting of individual colonies,…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.