Bacterial motility soft agar method does the test tell a microbiologist the number or distribution of the organelle?

I'm having trouble identifying the type of bacteria I have cultured. I know that it is gram-negative and cocci in shape, it grows wonderfully on the MSA plate but did not grow so well on the EMB plate, and it is non-motile. It has a yellow color but I'm just not sure what it is. It was obtained from a public area. Any ideas?

What are the possible reasons why culture plates may have too many colonies despite performing serial dilutions and OD600 readings?

Solve the identity of an unknown bacterial specimen by creating a dichotomous key.   Categories: Gram stain, Mannitol salt agar,MacConkey agar, Catalase, Oxidase, Casein hydrolysis, Nitrate reduction, Citrate test, Urease test, Sim Medium Streptoccocus ID: 1) Bile Esculin Test 2) Bacitracin Test  3) Blood Agar Plate NaCl Broth Staphylococcus ID: 1) Mannitol Salt Agar 2) DNA Hydrolysis 3) Coagulase Test 4) Novobiocin Test    Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes

A culture of Staphylococcus of unknown density is diluted as follows (letters represent each step): • 60 mL of a bacterial culture is diluted by adding 440 mL of water • 1000 microliters from (A) is added to 9 mL of water • Dilution (B) is used to prepare a 103 dilution in water • 100 ul from dilution (C) is plated to nutrient agar and incubated at 37°C Following incubation, colonies are uniformly distributed on the agar, and 102 colonies are counted on one half of the plate. 1. Express the total CFU/mL of the original culture in scientific notation. 2. If 10 ul is plated from dilution (C), how many colonies should grow on the plate? Edit View Insert Format Tools Table

Out of twelve tests that can be performed:  Gram Stain Wet Mount Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Indole Production Citrate Utilization Triple Sugar Iron Testing Catalase Test.  Which four tests are the best fit to help identify bacteria that is unknown? Possible bacterias that are being observed are:    Enterobacter aerogenes Escherichia coli Micrococcus luteus Proteus vulgaris Pseudomonas aeruginosa Salmonella typhimurium Serratia marcescens Staphylococcus aureus Staphylococcus epidermidis

What is meant by the word sterile? Why is aseptic techniquenecessary for successful maintenance of pure cultures in thelaboratory?

A microbiologist sets up a serial dilution to analyze bacteria in raw milk.   Tube 1 – 0.1 mL milk diluted in 9.9 mL sterile water Tube 2 – 0.1 mL milk from Tube 1 diluted in 9.9 mL sterile water Tube 3 – 1.0 mL milk from Tube 2 diluted in 9.0 mL sterile water   What is the dilution of raw milk in Tube 3?