Transcribe and translate the mutated sequence #1. Determine the consequence, if any, for each mutation. You ISN. Practice with both charts. Original DNA sequence: mRNA transcript: amino acids: Mutated DNA sequence #1: mRNA transcript: (Circle any changes) amino acids: TAC AUG METH TAC :: AUG :: UAG :: AAC Mutated DNA sequence #1 is an example of Substitution Insertion Deletion ACC UGG TRP ATC CGC TTG AAC ASN TTG 3 UGC :: Arg GCG CGC ARG GCG ACG :: Cys UGC CYS ACG :: UGA :: Start ACT ACT UGA STOP :: Stop :: Trp

What advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the use of a genomic library is necessary to provide information that a cDNA library cannot.

From the results of your BLAST search you can link to the GENE entry for one of your top hits. This link is located under the “Related Information” heading at the right hand side of each displayed alignment (i.e. scroll down to the“Alignments” section). Q1: How many exons and introns are annotated for this gene?  Q2: What is the function of the encoded protein?

Can you help with 1a please HELPFUL INFORMATION: When performing classical Sanger or "dideoxy" sequencing, you set up 4 parallel reactions per template to be sequenced from a specific primer, with each of the four reactions containing a different dideoxynucleotide, and then the four reactions were run in a separate, adjacent lanes on a gel. 1a. Why couldn't you combine all 4 dideoxynucleotides with the primer and the template and do the whole reaction in one tube, and then run the set of fragments produced by the reaction mixture on a single lane in an acrylamide gel?