While characterizing a mutation in a gene of interest, you discover that the mutation involves an insertion within the coding sequence of the gene. You suspect that the inserted sequence is a transposon and would like to determine which of the three major transposon families it belongs to. What sequence elements could be looked for within the inserted sequence that would help place it in one of the three families?

What is the minimal number of insertions/deletions of nucleotides that would result in a frameshift mutation?

Your advisor, a brilliant bioinformatician, has high regard for your intellect and industry. she suggests that you write a computer program that will identify the exons of protein- coding genes directly from the sequence of the human genome. In preparation for that task, you decide to write down a list of the features that might distinguish protein- coding sequences from intronic DNA and from other sequences in the genome. What features would you list?

The CRISPR-Cas9 system may be able to be used for somatic gene therapy to treat terminal genetic diseases such as Duchenne Muscular Dystrophy (DMD), which affects 1 in 3,500 male births worldwide. As we talked about in class, DMD results from a nonsense mutation that causes a premature stop codon in Exon 23 of the largest gene yet discovered in the human genome, the 79- exon dystrophin gene. Of the choices below, which would be most likely to restore the protein and muscle function? O A deletion of exon 23 O A deletion of intron 23 O A deletion of intron 23 and exon 23 O An insertion of a corrected exon 23 upstream of the mutant exon 23 O A deletion of exons 23, intron 23, and exon 24