While characterizing a mutation in a gene of interest, you discover that the mutation involves an insertion within the coding sequence of the gene. You suspect that the inserted sequence is a transposon and would like to determine which of the three major transposon families it belongs to. What sequence elements could be looked for within the inserted sequence that would help place it in one of the three families?

Q: Your company has decided to develop a PCR test that will be used to determine Huntington's disease.…

A: The polymerase chain reaction (PCR), is one of the most useful molecular biology techniques used to…

Q: Recombination signal sequences are conserved heptamer and nonamer sequences that flank the V, J, and…

A: Introduction: RAG 1 & 2 are the recombination activating genes that encode a portion of a…

Q: 1. a)What would happen if the aminoacyl tRNA synthetase responsible for charging alanine tRNAs also…

A: The polymerization of amino acids according to the codons on mRNA to form proteins is known as…

Q: A eukaryotic gene has two introns and three exons. The first intron closest to the promoter is 157…

A: To draw the structure of the hybridized mRNA and estimate the size of the protein coded for by this…

Q: After Drosophila DNA has been treated with a restriction enzyme, the fragments are inserted into…

A: a) By preparing a probe using the actin amino acid sequence or using the actin gene sequence known…

Q: DNA mRNA Amino Acid DNA mRNA Amino Acid DNA mRNA Amino Acid DNA mRNA Amino Acid CAA 105 CCG 154 AAG…

A: In case of DNA 4 bases are A,T,G,CIn case of RNA during transcription these transcribe same as the…

Q: Human genomic libraries used for DNA sequencing are often made from fragments obtained by cleaving…

A: Genomic Libraries are a set of collections of genomic DNA data particular to a species/organism. The…

Q: You obtain the DNA sequence of a mutant of a 2-kb gene in which you are interested and it shows base…

A: the mutation is the change or error that is caused in the chromosome it is of a different type 1.…

Q: For insertion elements and simple transposition, what is the function of the inverted repeat…

A: Transposition A transposon also called a jumping gene is the DNA sequence that changes its position…

Q: Shown below is an R loop prepared for electron microscopy by annealing a purified eukaryotic…

A: An exon is any part of a gene that will encode a part of the final mature RNA produced by that gene…

Q: It has been noted that most transposons in humans and other organisms are located in noncoding…

A: Transposons: Transposons are also referred to as "jumping genes" in which they have the ability to…

Q: You are working with a newly discovered mutagen, and you wish to determine the base change that it…

A: Mutagens often cause alterations in the DNA by changing bases. Substitutions are common examples…

Q: To identify the following types of genetic occurrences, would acomputer program use sequence…

A: Introduction  With the advancements of Bioinformatics tools and sequencing techniques we can easily…

Q: When an EcoR1 fragment, which represents the coding region of a human gene X, is cloned into the…

A: Fusion proteins are created by combining two or more genes into a single gene. GST is a common…

Q: Will the exon missense mutations be more likely to be transitions or transversions? Define both in…

A: Introduction  A mutation is a change that occurs in the DNA sequence of an organism. DNA…

Q: A graduate student is trying to identify the gene coding for an enzyme found in a bacterial species…

A: Trinitrotoluene (TNT), a solid organic nitrogen chemical that is mostly utilised as an explosive and…

Q: Describe the outcome of a chain-terminator sequencing procedure in which (a) too few primers are…

A: Chain terminating sequencing is also known as Sanger sequencing which is based on amplification of…

Q: You are studying a mutation in mice, which acts dominantly. Mice that have only one copy of the…

A: Definition Mutations are changes in DNA sequence. it can result from DNA copying mistakes , made…

Q: protein. How would the protein produced from sequence #1 differ from the protein produced from…

A: The sequences provided are of a non-coding strand or template strand. mRNA is transcribed…

Q: You have created three different mutations in the histoneH1 protein (HISmut1, HISmut2, HISmut3), and…

A: Histone 1 is a type of protein involved in chromatin remodelling. It is a part of histone protein…

Q: In studies of frameshift mutations, Crick, Barnett, Brenner, andWatts–Tobin found that either three…

A: The mRNA (messenger ribonucleic acid) contains an ORF (open reading frame) which is a continuous…

Q: Tay-Sachs disease (TSD) is a fatal genetic disorder, that results in progressive destruction of the…

A: Insertion or deletion mutation causes frame-shift mutation, it changes the amino-acid sequence of…

Q: You are interested in finding out the function of a particular gene in the mouse genome. You have…

A: There are multiple ways to have a distinct character for the gene expression and function. And the…

Q: Why did geneticists believe, even before direct experimental evidence was obtained, that the genetic…

A: The amino acid sequence of proteins is determined by the sequence of nucleotides in deoxyribonucleic…

Mitochondrial Mutations

A mitochondrion is a cellular organelle that serves as the site for cellular respiration and energy production (ATP or Adenosine Triphosphate). It is a double-membrane-bound organelle and is only present in eukaryotic cells.