With a plate count average of 297 and a final dilution on the plate of 1:1,000 ; what is the viable number of cells in original culture (CFU/ml)?

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Q: Determine what percentage of the culture was living (viable) and what percentage was dead…

A: The word "CFU" in microbiology refers to a colony-forming unit, which is used to estimate how many…

Q: Why is the plate count method a determination of the number of viable cells?

A: The plate count method, also referred to as the viable count method or colony-forming unit (CFU)…

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Q: How would you prepare a 1:500 dilution? You cannot use more than 10mL per tube.

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Q: Calculate the CFU present in 150 mL water sample and b) dilution factor up to 5. Consider the…

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Q: Calculate the final dilution in the following dilution series:  a. 1 mL in  9 mL dilution & 100uL…

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A: Tube 1: Distilled WaterAppearance: Swollen cellsWater Movement: Arrows pointing into the cellsTube…

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Q: Please count the cells in grids 1, 3, 7, and 9. Calculate the cell density (a) and cell viability…

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Q: 4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes…

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Q: but why is the answers all x 1000 at the end? for example day 1 is 2.025×1000=2025CFU/ml. should…

A: Answer.) :- When working with colony-forming units (CFU) in micro biology, we often need to dilute…

Q: If you make a five-tube twofold dilution using 2 mL of serum, what is the concentration of serum in…

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Q: A further sample of blood was taken from the above individual and prepared for haemoglobin analysis.…

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Q: You prepared a 7x 10^5x dilution from your bacterial culture, plated 0.2 ml of it on a Petridish and…

A: A colony-forming unit (cfu) is a unit used in microbiology to estimate the number of viable bacteria…

Q: When doing a routine differential count for a CBC how many cells are counted?   a) 50 b 75 c 100…

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Q: Which of the following is the a-anomer? H но H Но H2C H ОН H2C- нн ОН со 4 1 2 1 Submit 3 -OH Н ОН…

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Q: If an OD=1.0 culture yielded a plate count of 2.2 x 107 cfu/mL. What is the cell count of an OD=0.5…

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Q: Upon dislodging cells from the plate, you had the following cell counts using 4 different squares of…

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Q: 1 ml of urine is added to 2 ml of water. 1 ml is plated. 81 colonies form. What is the CFU/ml of the…

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Q: Provided with the following data, compute the corresponding CFU/ml of the original culture. ssume…

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Q: If the 10-9 plate was counted to have 155 colonies on it, would the CFU/mL be 1.55*1011? If not,…

A: CFU  Colony forming unit it a method to calculate the number of microorganisms in a culture media.

Q: Using 25 μL of serum, how much diluent would be needed to create a 1:2 dilution? ______ μL

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Q: Wil completed a full plate titer assay and there were 25 plaques on his 10^-7 plate. What is the…

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Q: Describe the Ninhydrin test. What does Ninhydrin test detect?

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Q: In one square of a hemacytometer you count 55 cells.  What is the approximate concentration of cells…

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Q: Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you…

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Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.