With a plate count average of 297 and a final dilution on the plate of 1:1,000 ; what is the viable number of cells in original culture (CFU/ml)?

Q: The solution containing bacterial spores is heated in an autoclave. When the autoclave has reached a…

A: Given that          Number of spores after autoclave reaches 121 C (No) = 106 spores/ml…

Q: What are the expected problems in the centrifuge in case of imbalance of samples ? *

A: Centrifugation is a process of separation of molecules from mixtures by applying centrifugal force…

Q: What is the dilution factor in the task given if the total viable cells is 140 [28×5(squares)] and…

A: The dilution factor is a measure of how much a solution has been diluted or concentrated. It is…

Q: what does chroomatography observation mean

A: Column fractionation was developed in the 1890s as a technique of separating the various components…

Q: Why is the MPN test qualitative rather than quantitative?

A: introduction: The Most Probable Number (MPN) method, which replicates liquid broth growth in…

Q: What is the proper collection time period for a fecal fat specimen? 1) 24 hours 2) 48 hours 3) 72…

A: Fecal lipids come from four different places. They are lipids that are consumed but are not…

Q: Why is Hypertonic IV solution used to reduce brain swelling?

A: Osmosis is the process by which water molecule is moved from the area of lower concentration (high…

Q: Why should the cell density of the inoculum be as small as possible? Why can we not standardize the…

A: The process of introduction of microbes into a nutrient media or other similar environments which…

Q: Answer the below question about “Haemotocrit/Packed cell volume (PCV)” 1)What is this test? 2)How is…

A: Hemoglobin is defined as the protein that is present in the red blood cells and they play a major…

Q: Why Purified synthetic alcohol is preferred for level vial?

A: An alcohol is an organic compound that carries at least one hydroxyl functional group (-OH) bound to…

Q: What do you mean by ultrafiltration?

A: The excretory system is the system that performs excretion, the bodily process of discharging…

Q: Why is it important to eject a small amount of blood from the syringe sample onto a gauze pad before…

A: Blood gas analysis is a diagnostic test. It gives useful information regarding the respiratory and…

Q: USING THE CONVERSION TABLE, MAKE THE FOLLOWING CALCULATIONS. 1) 26 lb = 2) 15 kg = 3) 3 oz = 4)…

A: It's important for nurses to be knowledgeable of various measurement units and conversions in order…

Q: turned red after Zn Stayed colorless after Zn turned red after NI and NII Which bacteria possess…

A: Nitrate test is done in the organism to know where they can convert nitrate to nitrite or not. The…

Q: how to calculate Calculate PFU -plaque forming units

A: PFU ( plaque forming units) is  the Virus titer (Virus per ml).  A plaque-forming unit (PFU) is a…

Q: You counted 40 colonies on a plate in your dilution series. The plate was inoculated with 1.0ml from…

A: A microbial sample will have enormous amount of cells. To estimate this by calculation, the sample…

Q: If an OD=1.0 culture yielded a plate count of 2.2 x 107 cfu/mL. What is the cell count of an OD=0.5…

A: Answer: The relationship between colony-forming units per millilitre (cfu/mL) and optical density…

Q: What is the concentration of a 50% solution after completing three 1:10 serial dilutions?

A: Serial dilution is the process that reduces the concentration of a solution in an organised manner.…

Q: Why administer IM injections using the Z-track technique.

A: Medicines are frequently injected intramuscularly (IM) to deliver the medication straight into the…

Q: A solution of flycine and a solution of albumin were prepared with the same concentration (g/L). One…

A: The ninhydrin test is a chemical that is used to determine the presence of alpha amino groups in the…

Q: What is the principle of :Density gradient centrifugation technique that commonly used for the…

A: For mononuclear cell isolation, the density gradient centrifugation is performed

Q: procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (1)…

A: Aseptic technique is most commonly used in microbiology and biotechnology lab. It refers to the…

Q: A milk sample was tested for the presence of typhimurium. Using a 10 microliter calibrated loop, a…

A: Serial dilution is the technique to find the concentration of bacteria in a sample when there are…

Q: If you count 70 colonies from a urine culture obtained using 0.001ml calibrated loop, how many…

A: CFU or colony forming unit is an unit of measurement that is used to estimate the viable microbial…

Q: Briefly describe the rationale and method of color compensation in flow cytometry.

A: Flow cytometry is a versatile laboratory technique used to analyze and sort cells or particles based…

Q: performing reagent strip testin

A: Reagent strips are the thin peice of paper which are impregnated with chemical reagents. These…

Q: How much serum is needed to create 2μL of a 1:20 dilution

A: Question is related to drug calculation .  Detailed solution in step 2.

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.