Human Anatomy & Physiology (11th Edition)
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ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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The following figure shows the FBI-style analysis of the genomic DNA of 10 people (1-10), and also of hair found at a crime scence left by the murderer [***]. This analysis involves the PCR amplification of SSR loci, each from a different (nonhomologus) chromosome. The PCR primers are for each SSR locus are labeled with a unique fluorescent molecule. Some bands are thicker because relatively more of the corresponding PCR product was obtained. The figure has dots aligned on both sides to help you find the crucial bands; it will help to use a straight-edge as a guide. The numbers at right are the total number of copies of the SSR locus among the population of 11 samples.
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Are any of the loci on the X chromosome? If so, identify this (these) locus (loci) by color.
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- Why is taq polymerase used in pcr reactions.arrow_forwardThe following figure shows the FBI-style analysis of the genomic DNA of 10 people (1-10), and also of hair found at a crime scence left by the murderer [***]. This analysis involves the PCR amplification of SSR loci, each from a different (nonhomologus) chromosome. The PCR primers are for each SSR locus are labeled with a unique fluorescent molecule. Some bands are thicker because relatively more of the corresponding PCR product was obtained. The figure has dots aligned on both sides to help you find the crucial bands; it will help to use a straight-edge as a guide. The numbers at right are the total number of copies of the SSR locus among the population of 11 samples. e. What is the probability that any random male in USA would share the same genotype as the murderer (the match probability)? Assume that all 11 DNA samples analyzed in the diagram are together representative of the USA population as a whole. Show what numbers you would multiply to do this calculation.arrow_forward5' 3' O 60°C 5' O 95°C Primer 1 O 75°C ORF For your PCR reaction above, the primer set has a much higher AT (low GC) content than what would be considered normal. Due to this difference in AT content, the optimal annealing temperature for the primers is going to have to be altered from the "normal" temperature cycle: 95°C 60°C ⇒ 75°C Which of the temperatures will have to be altered to accommodate this difference the primer set? Primer 25¹ 3' 5' annealing witharrow_forward
- Perform the Megaprimer PCR procedure (in scheme A). Indicates the number of Primers required in the protocol and marks their location according to the location of the nucleotide to be mutated (the X represents the nucleotide to be mutated). A)arrow_forward1) you have a locus in your PCR reaction that is a DNA squence is on the fifth chromosome, is a single copy locus, and has a squcne ID# 818. What is a name according to ISFG nomenclature 2) in electrophoresis system, DNA migartes toward____ which is _____ charged.arrow_forwardThe ability to multiplex the PCR reactions used in STR analysis (many PCR amplifications occurringin the same tube at the same time) simplifies the DNA profiling technique a great deal. However,the analysis of the PCR products involves measuring only their size and their color, and this is allthe software needs to know in order to figure out which PCR products are from which locus. Howcan the analysis software tell all the various loci apart?arrow_forward
- A DNA sequence is shown below, which includes a gene as marked. You have the restriction enzymes SalI and HindIII available to you to excise the gene prior to its incorporation into a plasmid vector. Which would you use to excise the gene?arrow_forwardThe human gene for hemophilia is on the X chromosome. Below is a pedigree from a family afflicted with hemophilia. The man with blackened symbol (III-1) has hemophilia. To help with genetic diagnosis, a probe that detects an RFLP (restriction fragment length polymorphism) on the X chromosome is used. This probe detects either a 7 kb restriction enzyme fragment or 3 kb and 4 kb restriction enzyme fragments. The RFLP pattern for all the members of the pedigree is shown. The recombination distance between the RFLP and the hemophilia locus is 40%. What is the chance that their unborn child (III-7) is affected? 4 kb 3 kb Select one: a. 10% b. 20% C. 40% d. 60% e. 80% f. 90% g. None of these above II IIarrow_forwardMicroarray hybridization is used mostly in transcript profiling or assaying DNA variation. Although the technology for establishing DNA microarrays was developed only recently, numerous applications have already been developed and their impact on future biomedical research and diagnostic approaches is expected to be profound. Give some examples of the practical use of this technique.arrow_forward
- All of the following are performed during restriction fragment length polymorphism analysis. 1. splitting of double-stranded into single-stranded DNA 2. gel electrophoresis 3. autoradiography 4. immersion in radioactive probes 5. digestion of DNA with restriction endonucleases 6. use of a positive charge to transfer single-stranded DNA from a gel to a membrane. The correct sequence of these operations is whatarrow_forwardMatch the following Conjugation terms with the most appropriate description in the image: F- recombinant F- strain F+ strain HFR strainarrow_forwardThe current state-of-the-art in forensic DNA profiling involves the PCR-amplification and analysis ofshort tandem repeats, STRs, in the human genome. This approach has many distinct advantages.Please list and explain three of those advantages.arrow_forward
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