In two independent PCR reactions, reaction A took 15 standard PCR cycles to obtain 1.31 × 108 copies (N) of the targeted gene, and reaction B took 18 standard PCR cycles to obtain 1.31 × 108 copies of the targeted gene. Assume the PCR reactions were not limited by any factors, compute the template DNA gene copies (No) in reactions A and B. Round up your answers to integers. No in the Reaction A: No in the Reaction B:

In the protocol for extracting DNA, what is the purpose of the clear shampoo in the DNA extraction buffer? In the protocol for extracting DNA, what is the purpose of the clear shampoo in the DNA extraction buffer? Shampoo contains detergents, that help to break down lipids in cell walls, thus freeing the DNA. Shampoo contains detergents, that help to break down proteins in cell membranes, including the nuclear membrane, thus freeing the DNA. Shampoo contains salts, that help to break down lipids and proteins in cell membranes, including the nuclear membrane, thus freeing the DNA. Shampoo contains detergents, that help to break down lipids in cell membranes, including the nuclear membrane, thus freeing the DNA.

PCR is an exponential copying of the template strands and can be represented by the function: y = a * 2n, where a is the initial number of template copies and n is equal to the number of cycles PCR has gone through.  How many DNA fragments would be produced after: 15 cycles? 13 cycles with 13 starting template strands? 29 cycles with 32 starting template strands?

Taxol is a compound used in cancer treatment. You are working for Genentech on a project to optimize the production of taxol purified from recombinant E. coli bacteria.  You have two new strains of SuperGro E. coli:  Strain A and Strain B, that you have engineered to express taxol. You want to know which of the two SuperGro E. coli strains is better to use for purifying taxol based on the amount you purify (measured by final concentration of protein in mg/mL). You also want to know which growth media (LB Media or SOC Media) results in a higher amount of purified taxol. You collect data and plot the average final concentration of taxol from each experimental condition in the graph below. Use the approach we discussed in class and write your analysis and interpretation of the data (describe the graph, describe the data, and interpret the data). Make sure to give clear and complete descriptions.  A. Describe the graph: B. Describe the data: C. Describe the interpretation: