For each of the pLM160 plasmid elements listed below: define the component AND briefly discuss the functional significance of this component for recombineering in Chlamydomonas reinhardtii. a. APHVIII b. Beta2-tubulin prom c. ccdB

Human Anatomy & Physiology (11th Edition)
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ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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For each of the pLM160 plasmid elements listed below: define the component AND briefly discuss the functional significance of this component for recombineering in Chlamydomonas reinhardtii. a. APHVIII b. Beta2-tubulin prom c. ccdB
5' cloning primer
annealing site
3' cloning primer
annealing site
ccdB
ccdB
term
ccdB
CrVenus YFP
prom
KanR2
3xFLAG
prom
PSAD
3'UTR
KanR2
PLM099
5356 bp
HSP70A/RBCS2
prom/5'UTR
KanR2
term
RBCS2
intron
p15A
origin
APHVIII
(paromomycin)
I-Scel recognition site
APHVII (hygromycinⓇ)
PLM161 and 162
BLE (zeocinⓇ)
PLM459
RBCS2 PLM099 and 160
3'UTR
m Turquoise 2-3xHA
PLM459
mScarlet-i-3xFLAG
PLM162
mNeon Green-3xFLAG
PLM160
Beta2-tubulin
prom
Transcribed Image Text:5' cloning primer annealing site 3' cloning primer annealing site ccdB ccdB term ccdB CrVenus YFP prom KanR2 3xFLAG prom PSAD 3'UTR KanR2 PLM099 5356 bp HSP70A/RBCS2 prom/5'UTR KanR2 term RBCS2 intron p15A origin APHVIII (paromomycin) I-Scel recognition site APHVII (hygromycinⓇ) PLM161 and 162 BLE (zeocinⓇ) PLM459 RBCS2 PLM099 and 160 3'UTR m Turquoise 2-3xHA PLM459 mScarlet-i-3xFLAG PLM162 mNeon Green-3xFLAG PLM160 Beta2-tubulin prom
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