Pair the appropriate item with its purpose in the production of insulin a DNA containing the gene for insulin. b Restriction enzymes and DNA ligase c PBR322 (a plasmid) d Lac promoter (regulatory sequence) e IPTG Triggers gene expression Provides sequences needed for RNA polymerase to bind and transcribe. Provides a means of getting the DNA into the bacterium and keeping it there. Allows DNA to be cut and put back together in order to produce the needed construct. Contains the sequence the bacterium needs in order to produce insulin.

When choosing a "first line" drug to treat an infection, the most important characteristic would be? the specific mechanism of action bactericidal broad spectrum low toxicity (fewest side effects) In what cell type can both transcription and translation occur simultaneously? both prokaryotic and eukaryotic cells neither prokaryotic and eukaryotic cells prokaryotic cells eukaryotic cells

Translational control of gene expression in humans regulates the synthesis of: RNA from an RNA template protein from an RNA template DNA from a DNA template protein from a DNA template RNA from a DNA template

Which of the following would you expect to happen if there were a mutation in the Iron Response Element (IRE) that prevented IRE-binding proteins from binding to the IRE.  The ferritin gene will only be translated with a low concentration of iron The ferritin gene will only be translated with a high concentration of iron The ferritin gene will be translated, regardless of the concentration of iron, The ferritin gene will not be translated, regardless of the concentration of iron