Table 2. Starter plate conditions. Starter Plate Bacterial Cas9 DNA Repair System SGRNA Donor Plate Additives Colony Color Template DNA IX IPTG, X-gal Blue OFF IX/ARA IPTG, X-gal, Blue ON arabinose A. Using evidence from Table 2, explain in complete sentences why the bacterial colonies on the starter plates are blue. B. If the bacteria on the starter plates did NOT have a functional lacZ gene, what color would you expect the colonies to be? C. Explain how the differences between the IX and IXIARA starter plates may influence gene editing in the laboratory activity.

Table 2. Starter plate conditions. Starter Plate Bacterial Cas9 DNA Repair System SGRNA Donor Plate Additives Colony Color Template DNA IX IPTG, X-gal Blue OFF IX/ARA IPTG, X-gal, Blue ON arabinose A. Using evidence from Table 2, explain in complete sentences why the bacterial colonies on the starter plates are blue. B. If the bacteria on the starter plates did NOT have a functional lacZ gene, what color would you expect the colonies to be? C. Explain how the differences between the IX and IXIARA starter plates may influence gene editing in the laboratory activity.

Name: a , b , c question Table 2. Starter plate conditions.StarterPlateBacte
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Description: a , b , c question Table 2. Starter plate conditions.StarterPlateBacterialCas9DNA RepairSystemSGRNADonorPlateAdditivesColony ColorTemplate DNAIXIPTG, X-galBlueOFFIX/ARAIPTG, X-gal,BlueONarabinoseA. Using evidence from Table 2, explain in complete sen

Biology: The Unity and Diversity of Life (MindTap Course List)

14th Edition

ISBN:9781305073951

Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Chapter8: Dna Structure And Function

Section: Chapter Questions

Problem 1DAA: HersheyChase Experiments The graph shown in FIGURE 8.5 is reproduced from an original 1952...

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Arial BIUA 11 + .. | I 1 I 3 I 4 i.) Fill in the table for each of the E. coli: (0) = No Activity (+) = Basal Activity and (+++) = High Activity E. coli chromosome F' Plasmid B-gal activity? Permease activity? When When When When Glucose is Lactose is Glucose is Lactose is present present present present a.) I+ P+ O+ Z+ Y+ Inone +++ +++ b.) I^[S] P+ O+ Z+ Y+ none c.) I+ P+ O^[c] Z+ Y+ none d.) I+ P+ O- Z- Y+ none e.) I+ P+ O+ Z+ Y+ I^[S] P+ O+ Z+ Y+ f.) I^[S] P+ O+ Z- Y+ I+ P+ O^[c] Z+ Y- g.) I^[TB] P+ O+ Z+ Y 1+ P+ O^[c] Z- Y+ h.) I+ P+ O^[c] Z+ Y- I+ P+ O+ Z** Y+ i.) I^[TB] P+ O^[c] Z+ Y- 1+ P+ O+ Z- Y+ Z** is a polar mutation ii. ) If the lac operon in 'a' carried a mutation in the CAP binding site that rendered it nonfunctional, how would that affect the level of ß-galactosidase protein activity with and without lactose present, why? MacBook Air 000
Staining characteristics of some bacteria. Please help me. I'm having trouble exactly figuring this.
HersheyChase Experiments The graph shown in FIGURE 8.5 is reproduced from an original 1952 publication by Hershey and Chase. Bacteriophage were labeled with radioactive tracers and allowed 10 infect bacteria. The virusbacteria mixtures were then whirled in a blender to dislodge any viral components attached to the exterior of the bacteria. Afterward, radioactivity from the tracers was measured. FIGURE 8.5 Detail of Alfred Hershey and Martha Chases 1952 publication describing their experiments with bacteriophage. Infected bacteria refers to the percentage of bacteria that survived the blender. After 4 minutes in the blender, what percentage of each isotope was extracellular?
HersheyChase Experiments The graph shown in FIGURE 8.5 is reproduced from an original 1952 publication by Hershey and Chase. Bacteriophage were labeled with radioactive tracers and allowed 10 infect bacteria. The virusbacteria mixtures were then whirled in a blender to dislodge any viral components attached to the exterior of the bacteria. Afterward, radioactivity from the tracers was measured. FIGURE 8.5 Detail of Alfred Hershey and Martha Chases 1952 publication describing their experiments with bacteriophage. Infected bacteria refers to the percentage of bacteria that survived the blender. How did the researchers know that the radioisotopes in the fluid came from outside of the bacterial cells and not from bacteria that had been broken apart by whirling in the blender?
HersheyChase Experiments The graph shown in FIGURE 8.5 is reproduced from an original 1952 publication by Hershey and Chase. Bacteriophage were labeled with radioactive tracers and allowed 10 infect bacteria. The virusbacteria mixtures were then whirled in a blender to dislodge any viral components attached to the exterior of the bacteria. Afterward, radioactivity from the tracers was measured. FIGURE 8.5 Detail of Alfred Hershey and Martha Chases 1952 publication describing their experiments with bacteriophage. Infected bacteria refers to the percentage of bacteria that survived the blender. The extracellular concentration of which isotope increased the most with blending?
HersheyChase Experiments The graph shown in FIGURE 8.5 is reproduced from an original 1952 publication by Hershey and Chase. Bacteriophage were labeled with radioactive tracers and allowed 10 infect bacteria. The virusbacteria mixtures were then whirled in a blender to dislodge any viral components attached to the exterior of the bacteria. Afterward, radioactivity from the tracers was measured. FIGURE 8.5 Detail of Alfred Hershey and Martha Chases 1952 publication describing their experiments with bacteriophage. Infected bacteria refers to the percentage of bacteria that survived the blender. Before blending what percentage of each isotope. 35S and 32P, was extracellular (outside the bacteria)?
HersheyChase Experiments The graph shown in FIGURE 8.5 is reproduced from an original 1952 publication by Hershey and Chase. Bacteriophage were labeled with radioactive tracers and allowed 10 infect bacteria. The virusbacteria mixtures were then whirled in a blender to dislodge any viral components attached to the exterior of the bacteria. Afterward, radioactivity from the tracers was measured. FIGURE 8.5 Detail of Alfred Hershey and Martha Chases 1952 publication describing their experiments with bacteriophage. Infected bacteria refers to the percentage of bacteria that survived the blender. Do these results imply that viruses inject DNA or protein into bacteria? Why or why not?
Escherichia coli Mycobacterium phlei Bacillus Micrococcus subtilis luteus A B Figure 1: Agar Slant Cultures of Bacteria (Gary E. Kaiser, Ph.D.- Professor of Microbiology) . Observe and describe the colour of the slant cultures (A-D) in fig 1. ( . Define the following terms: pure culture, sterile medium, inoculum, aseptic technique, and colony. What is the name of the cultures that you used . Where they gram negative or positive Define the following terms: psychrophile, mesophile, thermophile, and hyperthermophile.
A B Which of the bacterial colonies shown (A or B) was nonlethal when injected in mice? А В
In lab we learned a technique that helped us to visulize individual colonies of bacter 1. Describe this technique. 2. What do you expect the resutls to look like? Be specific. 3. How can this technique help you to determine if your culture is contaminated? For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac). BIUS Paragraph I +] F H Ix X ABC † ( O K₂ KN Q V Arial sè "Ω Θ A 4 10pt EE 88 A Click Save and Submit to save and submit. Click Save All Answers to save all answers. 描く前 X² X₂ 3 由用目

PART C (cont'd) Which of the following steps (on this page and the next page) would be necessary to determine the most effective antibiotic for a bacterial infection using the Disk Diffusion Assay? Select three necessary steps and three unnecessary or incorrect steps, and explain why it is or is not necessarv/correct. Collect a sample of bacteria from the infected patient. Collect samples of bacteria from the bathroom taps in the patient's room. Collect samples of bacteria from other patients who seem to have the same infection. Soak disks in many different types of antibiotic. Soak disks in the antibiotic you have the biggest supply ot. Label the disks with the type of antibiotic you soaked them in.
PART C (cont'd) Which of the following steps (on this page and the next page) would be necessary to determine the most effective antibiotic for a bacterial infection using the Disk Diffusion Assay? Select three necessary steps and three unnecessary or incorrect steps, and explain why it is or is not necessarv/correct. Collect a sample of bacteria from the infected patient. Collect samples of bacteria from the bathroom taps in the patient's room. Collect samples of bacteria from other patients who seem to have the same infection. Soak disks in many different types of antibiotic. Soak disks in the antibiotic you have the biggest supply ot. Label the disks with the type of antibiotic you soaked them in. • Leave the petri dishes uncovered while the bacteria grow. , cover the petri dishes while the bacteria grow. Treat the bacterial culture with a disinfectant before spreading on the agar plate Wear personal protective equipment (gloves, mask, lab coat) while performing the procedure Wear…