wwwwwwThe total number of cells in a culture is counted usingdye trypan blue and is found to be 3.8 x 106 cells/mL.The culture is diluted 1:22 and then 200μl seeded perwell into a 96 well plate.What is the final cell density per well?ssibility: Good to goMacBook Air

In biological experiment, Cell culture is an important technique, where cells grow under optimum…

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Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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A strep culture is sampled at two points in time, 1 hour and 10 minutes apart. 3.2 x 105 cells/ml are found in the first sample, and 1.8 x 107 cells/ml in the second sample. What was the generation or doubling time (in minutes)?
Design a serial dilution of a 25-gram cheese sample to achieve a final dilution of 10-5 using 90 ml blanks. Show how you would plate out 102 through to 10-6
B) A total of 2.5 x 105 cells were harvested from the patient. You need the cells to fill a scaffold with dimensions of 20mm x 20mm x 4mm at a final density of 2 x 104 cells/mm³. After culturing the harvested cells, you found that they have a doubling time of 36 hours. Given the growth rate is proportional to the number of cells, determine the time it will take to achieve the desired cell count. Show your work.
The following is result from a standard plate count. What is the cell density of the undiluted culture? Please follow all SPC rules. (TNTC: too numerous to count)
Refer to the following diagram: 100 ul 100 uL 100 pl 100 µL 100 pl 100 pL 100 pl Volume to transfer A B C D E F G 900 uL in each tube 10 102 10 10 10 10 10 Dilutions E.CoN culture Volume of the diluted sample plated 100 ul 100 μL 100 pL 100 ul Volume of the 10 ml 10 ml original sample plated 10 ml 10 ml Plate D Plate E Plate F Plate G Given that Plate D has too many colonies to count, and Plate G has no colonies at all as a result of serial dilution, draw out all four plates and demonstrate what they might look like (assuming serial dilution has been performed correctly). Based on your drawings, identify the number of CFU/ml for Plate E and Plate F. Show your calculations.
What was the concentration of bacteria (CFU/mL) in the original suspensions used for dilutions and plating below: Dilution Amount plated # of colonies counted 10-4 1.0 mL 271 10-5 0.1 mL 36 10-6 1.0 mL 9
The plate with 45 colonies was inocculated with 100 µL Volume of a 10^-8 Dilution. Given the formula OCD = CFU/V x D, what as the Original Colony Density?
Given the following media recipe, pick the terms that apply (pick as many as are applicable). 10.0 g Meat extract 10.0 g Peptone 5.0 g Sodium chloride 15.0 g 1.0 L Agar Water Once media is cool, add 6% sterile sheep's blood Defined Complex Minimal Selective Differential
How to calculate the percentage of phage that was filtered using a 0.45 um filter? PFU rep 1- 121 PFU PFU rep 2- 102 PFU Volume stock plated- 0.1 mL Average concentration= 5.6 x 109 PFU/mL.
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Calculate the concentration of bacteriophage in the original culture from the following data. Be sure to include units. Show dilution factors for each test tube. Show the final dilution factor for test tube number 4. Show all your math. 0.1 ml 0.01 ml 0.001 ml 0.1 ml 1.0 ml plated 9.9 ml 9.99 ml 9.999 mi 9.9 m plaques Original Culture Test Test Test Test Tube #1 Tube #2 Tube #3 Tube #4 Dilution Factor: Concentration of Phage:

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The total number of cells in a culture is counted using dye trypan blue and is found to be 3.8 x 106 cells/mL. The culture is diluted 1:22 and then 200μl seeded per well into a 96 well plate. What is the final cell density per well?