The total number of cells in a culture is counted using the trypan blue exclusion assay and is found to be 6.8 x 106 cells/ml. Each well in a 6 well plate requires 2 x 105 cells. How should the solution be diluted so that 1ml can be added to each well?

Q: define the following:               Mental disorder:               Neurotransmitters:…

A: A wide variety of mental health conditions—disorders which impact your emotions, thinking, and…

Q: Calculate the CFU/ml of leuconostoc in the brine for the 4 different days. Show your calculation

A: CFU/ml stands for "colony forming units per milliliter." It is a unit of measurement used in…

Q: What is the principle of freezing of cell lines and primary cells.Describe the general protocol for…

A: Cell culture is a procedure in which cells (either animal or plant cells) are separated from an…

Q: If there is a sample having 3.5x10^5 CFU/ml, and this cell count came from a sample of the original…

A: CFU stands for colony-forming unit that gives rise to a particular colony. It is generally assumed…

Q: There are two cultures of yeast cells in the pictures, one has been incubated for 6 hours and one…

A: Let's say, the yeast cell culture which was incubated for 6  hours as  = sample A the yeast cell…

Q: Fill in the gaps in the paragraphs below:  Blood pressure can be regulated by the nervous system, in…

A: The two parts of the autonomic nervous system are- sympathetic and parasympathetic. The sympathetic…

Q: A culture of E. coli has a concentration of 5 x 108 cells/mL. How many times do you have to dilute…

A: Given: 1. Number of colonies: 2502. No. of bacteria in culture: 5 X 108 3. Volume plated: 0.1 ml 4.…

Q: In the ELISA, the pH the coating buffer was 9.6 whereas the pH of the sample buffer (PBS based) was…

A: Coating buffer in ELISA is a mix of sodium carbonate and bicarbonate at ph 9.4. This gives charge to…

Q: What would your final dilution be if you transferred 0.1 mL of a 10-6 dilution and added it to a…

A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…

Q: how many coliform colonies would you expect to see on your 1:10 plate? Your 1:100 plate? Explain.

A: Introduction Coliform is the gram-negative, non-spore-forming and motile or non-motile bacterial…

Q: If these cells are actually Gram +, which of the following likely occurred during the staining…

A: The Gram's method may be a standard laboratory method wont to divide bacterial species into two…

Q: Explain how gel filtration chromatography works. What type of gel will you used when the protein…

A: Chromatography is a process for separating components of a mixture based on their differential…

Q: Since higher concentration colcemid will result in shorter chromosome, you want to      change your…

A: It is given that: Molar concentration of the stock solution: 10 ug/ml.  Desired dilution…

Q: What is the result of this biochemical test in the gelatin deep?cWhich enzyme is this testing for?…

A: Gelatin, also known as gelatine is a translucent, tasteless flavorless component that is often made…

Q: A capillary tube of whole blood is collected and centrifuged to obtain a plasma sample. A 1 in 4…

A: Capillary tube is a cylindrical tube with a fixed diameter which may hold any type of gas, liquid,…

Q: Differentiate the following artificial media used in animal cell culture: serum-containing media…

A:

Q: A sample of water was enumerated using a counting chamber (haemocytometer) and 220 bacterial cells…

A: The viable plate count, or simply plate count, is a count of viable or live cells. It is based on…

Q: 4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes…

A: Calibration graphs are used to estimate the concentration of unknown sample by plotting it against…

Q: For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much…

A: Serial dilution is one of the fundamental techniques carried out in chemical and biology…

Q: You are working in a medical lab and receive a urine sample from a patient that is displaying…

A:

Q: The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial…

A: Colony forming unit refers to the measurement of the number of viable cells present in per ml of the…

Q: The PD# of your culture is 165. You plated 3745 cells per well. You have recovered 12,655 cells.…

A: The passage number of a cell culture is a record of the number of times the culture has been…

Q: Shown below is a set of cell culture plates for a plaque assay.  The assay was performed by…

A: Approach to solving the question: Detailed explanation:  Examples: Key references:

Q: uring incubation, prepare dilutions of the standard antiserum which constitutes the standard curve…

A: To prepare a standard curve for the assay, we need to dilute the standard antiserum to a range of…

Q: Discuss the role of the addition of ammonium chloride potassium lysius buffer in splenocyte culture…

A: Any form of white blood cell that is found in the spleen or has been separated from splenic tissue…

Q: A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL…

A: It is given that a culture of Staphylococcus is diluted as follows:1. The original 20mL of culture…

Q: You are given a bacterial culture which has a concentration of approximately 5.0 x 10^8 cells/mL.…

A: We are given a bacterial culture containing approximately 5.0 x 108 cells/mL. To identify the exact…

Q: You aseptically transfer 1 ml of your optional liquid culture into 99 ml of sterile water. What is…

A: The following are needed for calculating the dilution factor  1) Original volume of  solution we…

Q: As shown in this diagram, you perform a ten-fold serial dilution of a culture to determine the…

A: The number of microorganisms that are able to divide and form new colonies is called a…

Q: Briefly explain how you could use the color of the Coomassie assay sample solution to determine if…

A: Colorimetric protein tests, for example, the Coomassie blue G-250 color (Bradford) examines, are…

Q: You want to create a 10-fold dilution . You have a saline tube with a volume of 450ul. How much of…

A: According to this theory, a diluted solution's volume will be 10 times greater than its stock…

Q: 1 trpt cells trp- cells + DNase 3 2 trp cells DNA 4 TSA plate 1 trpt cells trp- cells + DNA 3 2 trp…

A: In scientific experiments, a control is an experimental group or sample that is used as a comparison…

Q: You need to prepare medium for your culture cells. Your salt solution is supplied at a 10X…

A: The question asks to find the final concentrations of the following in 5 L of media:The salt…

Q: Which of the following are positive for the production of indoor from the amino acid tryptophan?

A: indole is produced by reductive deamination from tryptophan through the intermediate molecule indole…

Q: What is the color result of 1% solution of casein, Albumin, Gelatin in ninhydrin test? Include…

A: Ninhydrin test: Chemical test to detect presence of ammonia, primary/secondary amines, or amino…

Q: where 5 nutrient agar pates per female student and 3 agar slants per males student are needed to…

A: It is given that 5 nutrient agar plate prepared by per female so Seven female will prepare=7*5=35…

Q: You want to subculture your cells from T25 flask to 96-well plates. You first collected your cells…

A: A hemocytometer is an instrument that is commonly used to count the number of cells present in a…

Q: What does a white colony indicate during blue-white screening? Explain how the color is formed.

A: Gene transfer is a method in which genetic material is transferred from one bacteria to the another.…

Q: Biochemical and differential tests can be incorporated into growth media or performed on isolated…

A: It is required to provide three examples of differential test that is incorporated into growth media…

Q: A serial dilution of overnight E.coli culture was performed by pipetting 1ml of a bacterial culture…

A: A growth medium is intended to promote the growth of a variety of microorganisms or cells by the…

Q: Refer to the provided image drawn by a student trying to plan out their serial dilution protocol.…

A: Colony forming unit (CFU)is a measure of viable bacterial cell. CFU/ml can be calculated using…

Q: give the respective the TOTAL DILUTION FACTOR of respective serial dilutions. show complete…

A: Introduction: A serial dilution is any dilution in which the concentration decreases by the same…