Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Transcribed Image Text:(a) To illustrate how the ADP phosphorylative capacity was measured, the diagram below on
the left shows a ³1P NMR spectrum of the arm of a control female subject at rest, and the diagram on
the right the changes measured in the creatine and phosphocreatine levels of the arm at rest and
exercise.
PCr
10 Exer
-cise
Exer
→→→Rest◄cise Rest
PCr/Pi 9
PCr
15
20
10
Time(min)
Isokinetic ergometer exercise of the control female
subject showing intervals of rest and exercise. To
obtain this graph, a ³1P NMR spectrum was col-
lected every 2 min to show the changes in the PCr
and Pi content of the arm skeletal muscle tissue.
NH₂
during
activity
+
ATP +
C=NH2
during
recovery
YATP
Pi
GATP
BATP
SP
15
0
10
15
20
25
5
ppm
31P NMR spectrum of the arm of a control sub-
ject at rest showing characteristic resonance
features for the phosphate residues of ATP,
phosphocreatine (PCr), and Pi. At rest the
PCr/Pi ratio is ordinarily ~ 8-9.
The synthesis of phosphocreatine is unfavorable. If dur-
ing extended periods of rest, e.g., sleeping at night,
what must be the ADP/ATP ratio if the resting level of
PCr/Cr is 30/1?
0™
-0-P=0
N-H
+
C=NH₂2+ ADP
CH3-N
CH₂
COO™
Phosphocreatine
CH3-N
CH ₂
COO™
Creatine
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