ou are a bacteriologist studying a pathogenic protein (the “BAD” protein) that contributes to diseases caused by Staphylococcus aureus. BAD functions as an ab heterodimer, and the a subunit (25kD) and b subunit (75kD) are held together by an electrostatic interaction between K in the a subunit and D in the b subunit. You are trying to dissociate the BAD subunits to prevent pathogenesis in the bacteria. Which mutation would you make to prevent the BAD subunits from forming a dimer? Assume neutral pH.

We use Lysozyme to break down the cell wall in E.coli to lyse the cells.  Why do we not use Lysozyme on mammalian cells?

Most hospitals use hand sanitizers that claim to “kill 99.99% of illness causing germs”. What are some possible reasons that 0.01% of germs (germs = microorganisms and viruses) not affected by hand sanitizer? (In your answer, you should state what hand sanitizer is made of, and the mechanism by which it kills “germs”.

In the Spring and Summer of 2020, mass protests erupted across the US in response to police violence against African Americans. At the same time, the US was experiencing a COVID-19 pandemic. Many public health experts wondered whether participating in these protests (exposure) would be associated with being diagnosed with COVID-19 (outcome). Imagine you have been hired to draft a plan to study this potential association. There are many parts of the discussion. Be sure to answer all questions. • Which study design would you choose? (Almost any design is okay). Explain the factors that influenced why you selected that one. Include some of the benefits/drawbacks of your selected study design and the benefits/drawbacks of ONE of the study designs you did not select. • Write your research question and make a hypothesis about the results. • Research question should be neutral. Do not include words such as "increases" or "decreases." • Hypothesis can include words such as "increases" or…

This question combines your knowledge of bacterial conjugationand the genetic regulation that directs thephage λ reproductive cycles. When researchers mix donor Hfr strains with recipient F– bacteria that are lysogenic for phage λ, the conjugated cells survive normally. However, if donor Hfr strains that are lysogenic for phage λ conjugate with recipient F– bacteria that do not contain any phage λ, the recipient cells often lyse, due to the induction of λ into the lytic cycle. Based on your knowledge of the regulation of the two reproductive cycles of phage λ, explain this experimental observation.

ATR is a protein kinase that is activated by double stranded DNA breaks. a) True Ob) False

In the Hershey Chase experiment, why was the localization of radio-labeled phage of macromolecules in the cytoplasm the reason that the genetic material identity was proved.

HELP!..

1. Precise words:Find the nonspecific terms in the following sentences. Replace the nonspecific choices with more preciseterms or phrases (It is not necessary to change the sentence structure).(i) All OVE mutants showed enhanced iP concentrations.(ii) Plants were kept in the cold overnight.(iii) To provide proof of concept for our hypothesis, we studied a virus in its host cell.(iv) The present paper reports on continuing experiments that were performed to clarify thissurprising effect.(v) The first transition state is a little lower in energy than the second transition state. 2. Simple words:Improve the word choice in the following examples by replacing the underlined terms or phrases withsimpler word choices (do not change the sentence structure).(i) These data substantiate our hypothesis.(ii) The difference in our results compared to those of Reuter et al. (1995) can be accounted forby the fact that different conditions were used.(iii) For the purpose of discussing cell migration we…

After running our protein samples on an SDS-PAGE gel, we will use a Coomassie-based dye (Gel Code Blue) to stain the gel. Based on your prior experience with Coomassie dye and understanding of its function, what do you predict this dye to be able to stain? A) Only protein tags B) Only DHFR protein C) Only recombinant proteins D) All proteins