in an indirect elisa procedure what enzyme is used?

Q: what is the procedure and materials you need to stain Staphylococcus aureus?

A: Staphylococcus aureus is a Gram-positive, round-shaped bacterium hat belongs to the Firmicutes and…

Q: Provide the function of the following in blotting techniques: Nitrocellulose paper  paper weight…

A: Function of Nitrocellulose paper in blotting:  • Nitrocellulose paper is used in protein blotting…

Q: Why is the plate count method a determination of the number of viable cells?

A: The plate count method, also referred to as the viable count method or colony-forming unit (CFU)…

Q: List and explain the Sterile Technique What's the difference between Medical vs. Surgical Asepsis?…

A: Aseptic technique or Sterile technique means using practices and procedures to prevent contamination…

Q: ELISA is used to detect viruses where the key reagent is?

A: Immunoassay is a technique that has the capability to detect even minute quantities of antigen or…

Q: Observe figure  and make note of the several steps in theindirect ELISA test. What four essential…

A: Indirect ELISA is an immunoassay in which the antigen is bound by the primary antibody which is then…

Q: Explain what could cause a false positive result and what could cause a false negative result in an…

A: ELISA stands for Enzyme-Linked Immunosorbent Assay. It is a laboratory technique that is commonly…

Q: Differentiate the following artificial media used in animal cell culture: serum-containing media…

A:

Q: direct fluorescent antibody, ouchterlony diffusion, ELISA) which do you think would work best for…

A: Blood grouping Blood grouping is the classification of the blood based on the type of antigen/s that…

Q: What are the four steps of an Elisa protocol

A: Enzyme-linked immunosorbent assay (ELISA)  is an immunological assay commonly used to measure…

Q: If the protein produced full length antibody what would be the next step after centrifugation?

A: Antibodies are protein related to immune system know as Immunoglobulin. Each antibody is made up of…

Q: Antigen Antibody Enzyme-labelled antibody Substrate This image represents a/an. direc ELISA sandwich…

A: Enzyme-linked immunosorbent assay (ELISA) is an immunological assay that is used in laboratories to…

Q: In the first panel of part (a), what is the purpose of the antibodies attached to the well?

A: Enzyme-linked immunosorbent assay (ELISA) is a disease detection method (AIDS) and it works on the…

Q: What are some issues around the development of antiviral agents?

A: Since the research pathway entails a laborious procedure of discovering different chemicals that…

Q: What is the role of horseradish peroxidase conjugate in the ELISA experiment?

A: Horseradish peroxidase  The enzyme horseradish peroxidase (HRP), located in horseradish's roots, is…

Q: What is the Gram stain test procedure? What important diseases are caused by the following bacteria:…

A: The Gram staining is the the basic staining procedure that is used to identify bacterial. This…

Q: Counterstaining with Hematoxylin and Eosin is an important step in which of the following…

A: Counter stains are stains that are that are in different contrast color to that of the primary…

Q: ELISAs require a number of steps to be completed in order for the test to work. Why do you need to…

A: Enzyme-linked immunosorbent assay is commonly known as ELISA where Ag-Ab interaction is monitored by…

Q: ELISA TEST WOULD PROVIDE ME THE PRESENCE OF ANTIGENS FROM THE INFLUENZA A VIRUS?

A: ELISA or Enzyme Linked Immunosorbent Assay is based on the principle of antigen-antibody…

Q: You performed an ELISA and upon looking at your results, you realized you torgot to coat the wells…

A: A key feature of the plate based ELISA is that capture protein that is antibody or antigen can be…

Q: Why do serial dilution? Does serial dilution make it easier to count bacteria on a spread/streak…

A: Dilution is the process of making a solution weaker or less concentrated.

Q: If the test samples are positive for the antigen in the ELISA we are using, what color will you see?…

A: ELISA:                     ELISA ~ Enzyme-Linked Immunosorbent Assay          In analytical…

Q: What are the steps in sandwich ELISA? Explain and provide a schemiatic diagram

A: Enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA) are both widely used as…

Q: What are the equipments/ materials used in imoobilizing and separating cells when using: spread…

A: Aseptic techniques refer to any method which is used to sterilize and maintain sterility of the work…

Q: Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?

A: In microscopic studies, the specimen needs to be prepared for an optimized vision under a…

Q: State the four steps of Microarray Anaylsis.

A: Using microarray analysis, it is possible to assess gene expression levels as well as recognise…

Q: HIV testing uses a(n) ________________ELISA. direct indirect

A: Antibody tests can take 23 to 90 days to identify HIV disease after exposure. Most quick tests and…

Q: Why is a positive and a negative control used in a Elisa experiment?

A: The enzyme-linked immunosorbent assay (ELISA) is a laboratory technique for detecting and…

Q: Describe how an ELISA test is performed, how the test works and provide examples of ELISA tests.

A: ELISA is an Enzyme-Linked Immunosorbent Assay, which is used to detect antibodies or antigens in the…

Q: What is the principle involved in the Acrolein test? What is its purpose?

A: Qualitative tests are the chemical or physical tests involved in identification of certain…

Q: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…

A: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…

Q: Label the immunohistochemistry-negative specimen to identify what tissue is present. Evaluate the…

A: Based on the primary observation, this picture appears to be mesenchymal organ cells of the kidney.…

Q: A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution…

A: A sample of your blood, urine, skin, or any part of your body will be taken during a bacteria…

Q: Difference between Antibody ELISA and Antigen ELISA

A: Introduction Enzyme-linked immunoassay (ELISA) is an acronym for enzyme-linked immunoassay.…

Q: What is the advantage of immunological staining over traditional staining? In what field of science…

A: Immunological Staining: It is referred to as an advanced level of staining method wherein an…

Q: explain the Immunofluorescence Staining Protocol

A: Immunofluorescence is a method of visualization of a specific protein or antigen in cells or tissues…