If you wanted to make an assay detecting SARS-CoV-2 virus presence, like the assay shown in the previous question, what would be a logical combination of things to include in your list of supplies to obtain? O rabbit anti-SARS-cov2 spike antibody, anti-rabbit IgG horseradish peroxidase-conjugated antibody, tetramethylbenzidine O rabbit anti-SARS-cov2 spike antibody, anti-mouse horseradish peroxidase conjugated antibody, tetramethylbenzidine O rabbit anti-SARS-cov2 spike antibody, anti-rabbit SARS-cov2 spike antibody, tetramethylbenzidine O SARS-cov2 spike, anti-rabbit IgG horseradish peroxidase-conjugated antibody, tetramethylbenzidine rabbit anti-SARS-cov2 spike horseradish peroxidase conjugated antibody, anti-rabbit horseradish peroxidase conjugated antibody, tetramethylbenzidine

If you wanted to make an assay detecting SARS-CoV-2 virus presence, like the assay shown in the previous question, what would be a logical combination of things to include in your list of supplies to obtain? O rabbit anti-SARS-cov2 spike antibody, anti-rabbit IgG horseradish peroxidase-conjugated antibody, tetramethylbenzidine O rabbit anti-SARS-cov2 spike antibody, anti-mouse horseradish peroxidase conjugated antibody, tetramethylbenzidine O rabbit anti-SARS-cov2 spike antibody, anti-rabbit SARS-cov2 spike antibody, tetramethylbenzidine O SARS-cov2 spike, anti-rabbit IgG horseradish peroxidase-conjugated antibody, tetramethylbenzidine rabbit anti-SARS-cov2 spike horseradish peroxidase conjugated antibody, anti-rabbit horseradish peroxidase conjugated antibody, tetramethylbenzidine

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter13: Artifacts And Noise In Medical Instrumentation

Section: Chapter Questions

Problem 7P

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Can you please check my answer and make sure it is correct. Question: Describe the two roles primers play in PCR. Answer: Primers are short sequences of single stranded DNA that mark both the ends of the target sequence to be amplified during PCR. One primer attaches to the top strand at one end (forward primer) and the other one attaches to the bottom strand at the other end of the segment (reverse primer). They are made specifically to attach to these sections of DNA because the nucleotides of the primers are complementary to the target sequence. Other than marking the starting and end points for PCR, primers are also necessary for the process because without them, DNA polymerase wouldn’t be able to synthesize the complementary strand of DNA because it can only add nucleotides to an existing piece.
Can you please check my answer and see if it is correct. Question: In addition to master mix, what else must be added to each PCR tube? Why? Answer: In addition to master mix, the primers must be added to the PCR tubes. Each tube is also reserved for one individual’s specific section of DNA. So not only do primers needed to be added that are made specifically for the person’s DNA, but the DNA itself from the suspect needs to be added to the PCR tubes (DNA template strand). We also must have a control which contains sterile water, and one PCR tube reserved for DNA from the crime scene.
What are the things to consider in making a good primer? Does temperature affects the primer design? Discuss briefly.
How we can select Tm value for the primers? Please answer at your own words.
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Our PCR samples already contain loading dye, but sometimes this isn’t the case. If your samples didn’t already contain dye and you wanted to load your PCR sample onto an agarose gel, you’d need to add loading dye to the proper concentration. There is a 6X loading dye available for use; how many µl of this loading dye will you add to 10 µl of your sample so that it is at a 1X working concentration? Show your work.
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Pedigree Analysis Is a Basic Method in Human Genetic: What does OMIM stand for? What kinds of information are in this database?
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what is the approximate map distance between the two genes? Show your work.