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Which biochemical test is this? Name the organism that will test positive for this. Was this an important test for gram negative bacteria? Which tube is positive and negative?
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- What part of the test tube changes color first (in Resazurin Reduction Test)?In the ELISA, the pH the coating buffer was 9.6 whereas the pH of the sample buffer (PBS based) was 7.4 a) Why was the pH of the coating buffer so different?b) What is “coating buffer” for an ELISA? Does the buffer molecule usedmake sense based on the desired pH?In an ELISA procedure the samples are incubated and the ELISA plate iscovered with parafilm and placed in a humidified chamber to preventevaporation of the small liquid volumes in the wells.c) How would your results change if you did not incubate in a humidifiedchamber?What is the chemical basis of the Xanthoproteic Test? Give the chemical reaction involved.