The Michaelis-Menten equation is often used to describe the kinetic characteristics of an enzyme-catalyzed reaction. Vmax [S] Km + [S] where u is the velocity, or rate, Vmax is the maximum velocity, K is the Michaelis-Menten constant, and [S] is the substrate concentration. A graph of the Michaelis-Menten equation is a plot of a reaction's initial velocity (o) at different substrate concentrations ([S]). First, move the line labeled Vmax to a position that represents the maximum velocity of the enzyme. Next, move the line labeled 1/2 Vmax to its correct position. 300 mas
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
![The Michaelis-Menten equation is often used to describe the kinetic characteristics of an enzyme-catalyzed reaction.
Vmax [S]
Km + [S]
where v is the velocity, or rate, Vmax is the maximum velocity. K is the Michaelis-Menten constant, and [S] is the
substrate concentration.
A graph of the Michaelis-Menten equation is a plot of a reaction's initial velocity (ro) at different substrate concentrations
([S]).
First, move the line labeled Vmax to a position that represents
the maximum velocity of the enzyme.
Next, move the line labeled 1/2 Vmax to its correct position.
Then, move the line labeled Km to its correct position.
Estimate the values for Vmax and Km-
Vmax=
µM/min
v (µM/min)
300
275
250
225
200
175
150
125
100
75
50
Km =
25
K
0 10
20
30
V
max
40 50
[S] (M)
1/2 V
max
Michaelis-Menten curve
60 70 80
90 100
HM](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F572db862-e509-4419-9ca2-fed87cd6c4f5%2Fd7c06194-c43d-4eda-8e8a-d9057f01428f%2Fpllugok_processed.jpeg&w=3840&q=75)
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