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- Fill up the two missing values inside the two circles knowing that the total reaction volume is 2mL. 1) Dilution fraction 2) Water Don't include units in your answer. Membrane Brillant blueR 1g/L Dilution Sample suspension fraction (μL) (μL) 1) Control ? 0 50 2) 1/200 ? 10 50 3) 1/100 0.01 20 50 4) 1/50 ? 40 50 5) 1/20 100 50 Phosphate pH11, 0.2M (ml) 1 1 1 1 1 Water (mL) 0.95 ? ? ?Calculation at the initial point of a complexation titration: What is the pSr value of a 50.00 mL solution of 0.01000 M Sr2+ (formation constant, Kf = 5.348 x 108) after the addition of 0.00 mL of 0.02000 M EDTA which was buffered to pH 11.00? Round your final answer to two decimal places to the right of the decimal point.PROBLEM 12.27 (a) Use Table 12.5 to calculate the osmotic pressure of the hemodi- alysis solution at 25 °C. (b) If the osmotic pressure of blood at 25 °C is 7.70 atm, what is the direction of solvent movement across the semipermeable mem brane in dialysis? (Blood to dialysis solution or dialysis solution to blood)
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- pH 7.54 PaCO2 28 mm Hg PaO2 45 mm Hg HCO3- 22 mEq/L. Please provide an IPAP, EPAP and FiO2 in ( values not words) Desired FiO2 = [PaO2 (desired) x FiO2 (known)] PaO2 (known)Release of drug from a 20mm gel cube (B) was tested by placing it in a large dissolution apparatus containing a buffered solution into which the drug (A) was extracted. 4 of the sides are sealed, while 2 opposite sides are not. If Xp = 0.4, and DAB = 1.8 x 10-6 cm2/s, what ist (hr)?110 Elodea in distilled water ELODEA IN DISTILLED WATER Draw what the Elodea cells looked like when placed in distilled water. Total Magnification = 1. Fill in the blank using one of the three tonicity terms: Distilled water is compared to the cytoplasm of the Elodea cells. X
- 1. 2 3. 4. 5. Study the following experimental method: • Enzyme solution: Alkaline phosphatase in 50mM Tris-HCl, pH=10 Buffer solution: 50mM Tris-HCl, pH=10 • Vitamin C • Acid Molybdate Method Prepare the 2 following test tubes: Add 100μl of the enzyme solution to 500μl of the horn sample. Add 2.4ml of the buffer solution and mix the solution. Add 100μl of the buffer solution to 500μl of the horn sample. Add 2.4ml of the buffer solution and mix the solution. Add 1ml Vitamin C, 1ml Acid Molybdate and 5ml water to the 2 tubes and mix. Leave the 2 tubes at room temperature for 20 minutes and read the absorbance at 670nm. a. b. Determination of the organophosphate concentration of a horn sample. A standard curve of phosphate will be provided. Determine the concentration of the organophosphate in the horn sample. Which of the following reagents will you include in the blank (negative control) for this experiment? Alkaline phosphatase O Buffer solution: 50mM Tris-HCl, pH=10 Vitamin C Acid…100 mL of 2.5 X NaOH solution from 50X NaOH solutionRuhemann’s purple/blue-violet coloration in Ninhydrin test