Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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- If decanoic acid (10 : 0) undergoes complete β-oxidation in mitochondria, how many net ATP is yielded? (NADH = 2.5 ATP; FADH2 = 1.5 ATP; Acetyl CoA = 10 ATP) Answer in number onlyarrow_forwardThis UCP1 protein allows the protons (H+) of the mitochondrial intermembrane space to enter the mitochondrial matrix without going through the ATP synthase. This bypass represents a shortcut for protons and uncouples (i.e. eliminates the interrelationship between) the following two processes: The entry of protons inside the mitochondrial matrix, following their concentration gradient. The phosphorylation of ADP into ATP by the ATP synthase. It is this uncoupling that gave the protein its name: uncoupling protein 1 (UCP1). The following figure shows the location of UCP1 when it is expressed (synthesized) in the brown adipose tissues (BAT) of hibernating mammals. Question 12 Based on the UCP1 protein structure shown earlier, and based on the figure above, what type of protein is UCP1? Question 12 options: a) A primary active transporter. b) A motor protein.…arrow_forwardHow many ATP are produced from the complete degradation of G3P using glycerol- three phosphate shuttle?arrow_forward
- The primary purpose of the aconitase step in Citric Acid Cycle is to: form the intermediate aconitate prepare the citrate molecule for oxidative decarboxylation act as a commitment and regulatory step produce isocitratearrow_forwardConcerning the citric acid cycle (TCA cycle), which of the following statements is false? The TCA cycle generates electron carriers Oxidation of pyruvate in the matrix of the mitochondria produces sixteen total electrons Substrate level phosphorylation in the TCA cycle produces 2 GTP per the oxidation of a single glucose molecule The fate of the carbonyl carbon of acetyl CoAin the TCA cycle is to be lost as CO2 in the second turn Oxygen is required to regenerate electron acceptorsarrow_forwardWhich step(s) of cellular respiration would be directly inhibited by high concentrations of NADH? How would this affect other steps of cellular respiration?arrow_forward
- During glycolysis, transition, and the citric acid cycle: How many ATP (or GTP) is made in each step? Why is CO2 made in some steps? (Hint: this is NOT from O2) Which steps make activated carrier molecules? What are they “carrying”?arrow_forwardWhen the antibiotic X is added to actively respiring mitochondria, several things happen: the yield of ATP decreases, the rate of O2 consumption increases, heat is released, and the pH gradient across the inner mitochondrial membrane increases. Does X act as an uncoupler or an inhibitor of oxidative phosphorylation? Explain the experimental observations in terms of the antibiotic’s ability to transfer K+ ions across the inner mitochondrial membrane.arrow_forwardThe four complexes of the electron transport chain use the energy of electrons stored in reducing agents to create a concentration gradient of protons (H*) across the mitochondrial inner membrane. Give the number of protons pumped into the intermembrane space by each of the four complexes: complex I: complex II: complex III: complex IV:arrow_forward
- Under aerobic conditions when glucose is limiting, with high ratios of NADH/NAD+ and ATP/ADP, as carbon-2 radiolabeled pyruvate is utilized for its carbon skeleton, which molecules would you expect to see significant radiolabeling in the liver? Select all that apply. (multiple answers) Glucose C-2 only Label is halved over many TCA cycles Oxaloacetate Glucose C-1 and C-6 Glucose C-2 and C-5 CO2 from TCA cycle shows some radiolabel Lactate C-2 for export Malate Pyruvate C-1arrow_forwardATP synthesis in mitochondria requires all of the following EXCEPT (select two answers) a hydrogen ion concentration gradient across the inner mitochondrial membrane the oxidation of dinucleotide molecules that act as electron carriers fermentation of pyruvate into lactate to regenerate NAD+ the reduction O2, forming H2O the breakdown of glucosearrow_forwardTaking into consideration glycolysis, the pyruvate dehydrogenase complex and the citric acid cycle, how many NAD+ molecules are reduced from a single molecule of dihydroxyacetone phosphate? 4 NAD+ are reduced 5 NAD+ are reduced 8 NAD+ are reduced 10 NAD+ are reduced None of the above answers are correctarrow_forward
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