Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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How many ATP are produced by substrate level phosphorylation ONLY in the complete oxidation of activated palmitate (C16:0 fatty acyl CoA) in a skeletal muscle cell
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- Unlike a rabbit, running all-out for a few moments to escape a predator, migratory birds require energy for extended periods of time. For example, ducks generally fly several thousand miles during their annual migration. The flight muscles of migratory birds have a high oxidative capacity and obtain the necessary ATP through the oxidation of acetyl-CoA (obtained from fats) via the citric acid cycle. Compare the regulation of muscle glycolysis during short-term intense activity, as in a fleeing rabbit, and during extended activity, as in the migrating duck. Why must the regulation in these two settings be different? Extended activity requires the highly efficient anaerobic metabolism of fats, rather than the less efficient aerobic metabolism of glucose. Extended activity requires the highly efficient aerobic oxidation of fats, rather than the less efficient anaerobic metabolism of glucose. Extended activity stimulates glycolysis because the concentrations of citrate and acetyl-CoA are…arrow_forwardWhat is the function of glyceraldehyde 3-phosphate dehydrogenase? catalyzes the transfer of a phosphoryl group from ATP to an acceptor oxidation of the alcohol to an aldehyde dehydration and dephosphorylation of GAP hydrolysis of GAP oxidation by NAD+ and formation of acyl-phosphatearrow_forwardif each NADH generates 3 ATP molecules and each FADH2 generates 2 ATP molecules, calculate the number of ATP molecules generated from one saturated 10-carbon fatty acid Determine the number of repetitions of the β‑oxidation spiral needed to completely degrade the fatty acid to acetyl-SCoAacetyl-SCoA (acetyl-CoA)(acetyl-CoA) . Calculate the ATPATP produced by the acetyl-SCoAacetyl-SCoA molecules in the citric acid cycle. Calculate the ATPATP produced from the FADH2FADH2 and NADHNADH produced from β‑oxidation. Add the ATPATP generated (from step 2 and 3), and subtract the ATPATP needed to activate the fatty acid.arrow_forward
- Efficiency of ATP Production in Muscle: The transformation of glucose to lactate in myocytes releases only about 7% of the free energy released when glucose is completely oxidized to CO2 and H2O. Does this mean that anaerobic glycolysis in muscle is a wasteful use of glucose? Explain.arrow_forwardFatty acid oxidation for energy yield occurs in the mitochondrial matrix, and for palmitate as one example, generates [FADN2, NADH, and Acetyle-CoA or FADH, GTP, and NADPH] , all of which can be converted to ATP (108 of them to be exact) via oxidative phosphorylation. This is a [greater or smaller] yield of ATP per carbon atom compared to glucose. Ketogenesis is a process where acetyl-CoA (including that from breakdown of fatty acids) is converted to ketone bodies under conditions where carbohydrates are [in excess or liminting] . Acetoacetate and D-beta-hydroxybutyrate are delivered from liver to the blood stream where they provide energy for [cardiac and skeletal muscle as well as brain or synthesis of glycogen] . [Insulin or glucagon] promotes ketogenesis by stimulating fatty acid export from adipose tissue.arrow_forwardName one reaction of glycolysis, one anaplerotic reaction, and one reaction of TCA cycle that acetyl-COA allostericqally regulates ( and declare whether it is an activator or inhibitor.)arrow_forward
- ATP, like ADP and AMP, is a competitive inhibitor of NADH binding to malate dehydrogenase. Provide a structural explanation for this inhibition.arrow_forwardDuring Krebs cycle, the conversion of isocitrate to alpha-keto glutarate involves all of the following reactions/mechanisms except Reduction of isocitrate Reduction of NAD+ Decarboxylation of isocitrate Oxidation of isocitratearrow_forwardLactic acid is a biproduct formed at the end of anaerobic respiration. This molecule is dangerous to cells of the body if it builds up too much. Despite this, the formation of lactic acid is very important. Explain why the process of anaerobic respiration is so important (providing reference to glycolysis, the role of coenzymes, and ATP formation).arrow_forward
- When regulating phosphofructokinase, why is there such extensive regulation of this reaction and enzymatic reactions, in general?arrow_forwardThe first step of gluconeogenesis involves the carboxylation of pyruvate and has a large negative delta G (i.e very exergonic). Explain how it is possible that this step can be exergonic if we know that adding CO2 onto molecules is fundamentally a destabilizing phenomenon?arrow_forward
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