Answer the correc optionnot need explanation need ASAP The following is the PCR reaction that is programmed in the thermocycler to amplify the cheek cell DNA:1. 95°C for 2 minutes2. 95°C for 30 seconds3. 58.1°C for 30 seconds4. 72°C for 45 seconds5. Repeat steps 2-4 thirty (30) times6. 72°C for 3 minutes7. Hold at 16°C indefinitelyWhich steps represent the denaturation, annealing and extension steps?O a. Step 4 = Denature; Step 6 = Anneal; Step 7 = Extend%3DO b. Step 3 = Denature; Step 4 = Anneal; Step 6 = Extend%3D!!C. Step 2 = Denature; Step 4 = Anneal; Step 6 = Extend%3DO d. Step 2 = Denature; Step 3 = Anneal; Step 4 = Extend

Polymerase chain reaction (PCR) is a method that is used to amplify a selected region of DNA and…

Answered: The following is the PCR reaction that…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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In a PCR reaction to amply a particular gene, 120 copy of the gene were initially used as DNA templates (this is the Nō). Theoretically, how many copies of this particular gene can be obtained after 25 PCR cycles? 4.03 × 10⁹ 2.01 × 10⁹ 1.02 × 10⁹ O 6.04 × 10⁹
The figure below illustrates the stages of production of useful medical products using recombinant DNA technology. Closely study the figure then answer the Activity 1 questions that follow: Human cell Bacterium 1. DNA Plasmid DNA Human insulin-producing gone 2. DNA is cut with restriction Bacterial DNA with human gone inserted enzymes. 3. Plasmid is reintroduced into bacterium. 4. Engineered bacteria multiply producing insulin. 5. Insulin is separated and purified to produce human insulin. Human insulin Itulin 6. Insulin injected into patient 1. What is a plasmid? Make a diagram of bacterial cell containing a plasmid. 2. How do you explain the selection of plasmids for carrying the desired gener 3. Follow the steps of human insulin production, then 4. Make a diagram for the production of growth hormone. first
The figure below illustrates the stages of production of useful medical products using recombinant DNA technology. Closely study the figure then answer the Activity 1 questions that follow: Human cell Bacterium 1. DNA Plasmid DNA Human insulin-producing gone 2. DNA is cut with restriction Bacterial DNA with human gone inserted enzymes. 3. Plasmid is reintroduced into bacterium. 4. Engineered bacteria multiply producing insulin. 5. Insulin is Human insulin separated and purified to produce human insulin. 6. Insulin injected into patient 1. What is a plasmid? Make a diagram of bacterial cell containing a plasmid. 2. How do you explain the selection of plasmids for carrying the desired gener 3. Follow the steps of human insulin production, then 4. Make a diagram for the production of growth hormone. first
Primer annealing is an important aspect of PCR. The annealing step of the cycle usually takes place at around 45-55°C and lasts for only 20-30 seconds. Which of the following statements best explains why the annealing time has to be so brief? OA. A longer annealing time would prevent primers binding to specific sites. OB. The short time frame prevents primers from binding to each other. OC. If the time were any longer, DNA polymerase would begin to denature. OD. The short time frame minimizes complementary template strands base pairing with each other. Reset Selection
You receive a tube containing 18.8 nmol of lyophilized (freeze dried) primers to be used in PCR. How many µL of water would you need to add to create a 100 µM solution?
(a) To illustrate how the ADP phosphorylative capacity was measured, the diagram below on the left shows a ³1P NMR spectrum of the arm of a control female subject at rest, and the diagram on the right the changes measured in the creatine and phosphocreatine levels of the arm at rest and exercise. PCr 10 Exer -cise Exer →→→Rest◄cise Rest PCr/Pi 9 PCr 15 20 10 Time(min) Isokinetic ergometer exercise of the control female subject showing intervals of rest and exercise. To obtain this graph, a ³1P NMR spectrum was col- lected every 2 min to show the changes in the PCr and Pi content of the arm skeletal muscle tissue. NH₂ during activity + ATP + C=NH2 during recovery YATP Pi GATP BATP SP 15 0 10 15 20 25 5 ppm 31P NMR spectrum of the arm of a control sub- ject at rest showing characteristic resonance features for the phosphate residues of ATP, phosphocreatine (PCr), and Pi. At rest the PCr/Pi ratio is ordinarily ~ 8-9. The synthesis of phosphocreatine is unfavorable. If dur- ing extended…
Imagine that you are a student in Alfred Hershey and Martha Chase’s lab in the late 1940s. You are given five test tubes containing E. coli bacteria infected with T2 bacteriophages that have been labeled with either 32P or 35S. Unfortunately, you forget to mark the tubes and are now uncertain about which were labeled with 32P and which with 35S. You place the contents of each tube in a blender and turn it on for a few seconds to shear off the phage protein coats. You then centrifuge the contents to separate the protein coats and the cells. You check for the presence of radioactivity and obtain the following results. Which tubes contained E. coli infected with 32P-labeled phage? Explain your answer. Tube number Radioactivity present in 1 Cells 2 Protein coats 3 Protein coats 4 Cells 5 Cells
Imagine that you are a student in Alfred Hershey and Martha Chase’s lab in the late 1940s. You are given five test tubes containing E. coli bacteria infected with T2 bacteriophages that have been labeled with either 32P or 35S. Unfortunately, you forget to mark the tubes and are now uncertain about which were labeled with 32P and which with 35S. You place the contents of each tube in a blender and turn it on for a few seconds to shear off the phage protein coats. You then centrifuge the contents to separate the protein coats and the cells. You check for thepresence of radioactivity and obtain the following results. Which tubes contained E. coli infected with 32P-labeled phage? Explain your answer.
Why are DNA samples that are to be separated by gel electrophoresis always loaded at the cathode end of the power source? The sequencer in this lab experiment using a thin capillary tube to perform gel electrophoresis. Explain how this accomplishes the same task as traditional flatbed electrophoresis.
Select all the following statements that are correct regarding agarose gel electrophoresis and restriction digests. Group of answer choices A higher percentage of agarose is used to distinguish between smaller pieces of DNA DNA fragments run from the positive electrode (where they are loaded) to the negative electrode Buffer is poured into the electrophoresis chamber AFTER the DNA has been loaded into the lanes. Larger DNA fragments migrate more slowly than smaller DNA fragments Loading dye is added AFTER restriction digests have been completed.
Answer the following questions related to PCR Bioinformatics for DNA Extraction using Why is it necessary to chelate the metal ions from the solution during the boiling/lysis step at 100 degrees celsius? What would happen if you did not use a chelating agent such as the InstaGene matrix? What is needed from the cells for PCR? What structures must be broken to release the DNA from a cell?
(a) Describe the actions with reason that should be taken under the following situations: (i) An unlabelled collection tube with a requisition for a V Leiden test is received in the laboratory. (ii) After PCR, the amplification control has failed to yield a product. (iii) DNA is stored overnight in a refrigerator set at 8oC but reads 14 oC. (iv) An isolated DNA sample is to be stored for at least 6 months. (v) The expiration date on a reagent has passed. (b) Explain briefly about genetic testing for new-born, including the importance of genetic testing and the disorder that genetic testing can detect.

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