Assay of Serum Alkaline Phosphatase. Serum component of blood is preferred to be use than plasma, why is this so? Explain
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Assay of Serum Alkaline Phosphatase. Serum component of blood is preferred to be use than plasma, why is this so? Explain
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- On blood agar plate. What hemolysis is this? (alpha, beta, gamma)B. COLOR REACTIONS. Give the ideal observation for the positive results of the following samples to complete the table below. Write NO RXN if the sample gives negative result for the given tests BIURET XANTHOPROTEIC NINHYDRIN SULFUR SAMPLES TEST TEST TEST TEST Formation of violet- Cysteine NO RXN colored product Formation of red- Tryptophan NO RXN colored solution Formation of Formation of violet- Keratin black colored product precipitate B - Alanine NO RXN NO RXNH. Consider the answers you provided in the questions above in order to answer the following question. You treat a suspension of Stabhylococcus aureus with lysozyme enzyme dissOved 25 mM Tris buffer (pH 7.4). After a 30-minute incubation, the cells lyse completely. You treat a suspension of Pseudomonas aeruginosa with the same lysozyme/Tris solution and get no lysis after a one-hour incubation. Why? Provide a detailed answer based on your understanding of
- PRECIPITATION OF PROTEINS 2. Give the rationale for the following: a) The use of picric acid in burns b) The use of tannic acid in diarrhea 2. List 5 agents (physical or chemical) which could be used to destroy the life of microorganisms by coagulating its protein.description of a the oxidase testDescribing pharacokrelic properties mechanisms of action, chirical application pharmacologic and toxic effects of: 1.Cardiac glycosIdes 2. Beta-agonists
- Explain how the Kirby-Bauer test works and what information it provides. Define minimum inhibitory concentration (MIC). Describe how the E-test works and what information it provides.In the ESTIMATION OF TOTAL SERUM CHOLESTEROL using ZAK AND HENLY’S METHOD, explain why should the serum stay clear? What are the implications of using a turbid serum sample?Explanation for Acrolein test equation
- piysion 3. The patient went to the clinic with complaints of dizziness, shortness of breath, palpitations and pain in the limbs, which sharply worsened after a short rest in the mountains. A reduced number of red blood cells were found in the patient's blood, as well as immature crescent-shaped cells and red blood cells. What are the causes of this pathology? Why did the discase worsen in conditions of low partial pressure of oxygen?What is the best anticoagulant for blood? Explain.Standard curves You are setting up a standard curve for lysozyme. You have a stock vial containing 2 mg/ml of lysozyme and you have 2 ml in the vial. You will want to have a minimum of 1 ml of each concentration for the spectrophotometer. How would you proceed? (Saline is added to make the various concentrations). Concentration Am't stock lysozyme or am't of a previous dilution (note which) Amount saline Total volume wanted 1.00 mg/ml 0.75 mg/ml 0.50 mg/ml 0.375 mg/ml