Why are DNA samples that are to be separated by gel electrophoresis always loaded at the cathode end of the power source? The sequencer in this lab experiment using a thin capillary tube to perform gel electrophoresis. Explain how this accomplishes the same task as traditional flatbed electrophoresis.

describe the two main parts of a plasmid DNA extraction and purification procedure including the purpose of each step

A student is trying to add 9.0 pmol of primer mix to a 20.0 µL PCR.  The primer mix is at a concentration of 50.0 µM, and the student determines that a serial dilution is required because directly adding the primer mix would require a volume less than 1.00 µL.  The student takes 1.40 µL of the primer mix stock and mixes it with 25.0 µL of ultrapure H2O.  Calculate the volume (in µL) of the intermediate solution required to add the correct amount of primer mix to the PCR.

You are given a tube containing 275 ng of purified PCR product (DNA) that is 1262 bp long. How many picomoles of PCR product are in the tube? The average molecular weight of a deoxynucleotide monophosphate is 328 g/mol.