An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper chromatography. The solvent is less polar than water. Which of these amino acids will have the highest Rf value and which the lowest? Explain.

How much agarose powder (g) would you use to make a 50 ml, 1.4% agarose gel?

A mixture of lipids containing phosphatidic acid, cholesterol, testosterone, and phosphatidylcholine was applied to a hydrophobic interaction chromatography column. The column was then washed with increasingly nonpolar solvents. IWhat order would the lipids be eluted from the column?

Using DEAE-cellulose as ion exchange resin, indicate the starting and ending pH for the narrowest experimental pH range used to separate an amino acid mixture consisting of Gln, Leu and Lys Starting pH: _____ Ending pH: _____

Scott test is one of the methods used for screening for Cocaine in Forensic Chemistry. The multi step method consists of a) reacting the sample drug with cobaltous thiocyanate (Co(SCN)2) b) dissolving of the formed precipitate by adding HCl  and c) extraction of coordination compound with Chloroform d) measurement  of  absorbance of extracted coordination compound at 627 nm    What is the type of chemical equilibria  involved in first step of the method? a.Solubility Product equilibria b.Bronsted Lowry acid – Bronsted Lowry base equilibria c.Complex ion formation d.Coordination compound formation

How do you prepare 500 mL pH 6 Citric acid/Phosphate buffer in the laboratory. Please explain briefly.

Arrange the following in order of increasing Rf on thin-layer chromatography: acetic acid, acetaldehyde, 2-octanone, decane, and 1-butanol.

A pharmacist receives a prescription for 60 g of a 0.75% w/w bexarotene gel. How many grams each of a 1% w/w bexarotene gel and gel base must be used?

You are preparing 1 Lt of TAE (Tris Acetate EDTA) gel running buffer from a 50x stock solution. How much 50 x TAE and how much water will you combine?

An aqueous solution contains 0.448 M dimethylamine ((CH3)2NH).How many mL of 0.240 M hydroiodic acid would have to be added to 150 mL of this solution in order to prepare a buffer with a pH of 10.500? ______________ml